LOCUS       BBa_K1401608            1849 bp    DNA     linear       16-Feb-2025
DEFINITION  pLac - BCD2 - tetR - YFP
ACCESSION   BBa_K1401608
VERSION     BBa_K1401608.1  
FEATURES             Location/Qualifiers
     misc_feature    1..200
                     /label=BBa_R0010
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_K783046
     RBS             213..296
                     /label=BCD2
     misc_feature    209..212
                     /label=MoClo Fusion Site B
     misc_feature    297..300
                     /label=MoClo Fusion Site C
     RBS             209..300
                     /label=BBa_K1114107
     CDS             309..973
                     /label=BBa_K1114202
     misc_feature    982..1712
                     /label=BBa_K1114208
     stem_loop       1732..1775
                     /label=stem_loop
     misc_feature    1721..1800
                     /label=BBa_B0010
     terminator      1721..1800
                     /label=BBa_B0010
     stem_loop       1816..1835
                     /label=T7 TE
     misc_feature    1842..1842
                     /label=stop
     polyA_site      1836..1849
                     /label=polya
     misc_feature    1809..1849
                     /label=BBa_B0012
     terminator      1809..1849
                     /label=BBa_B0012
     terminator      1721..1849
                     /label=BBa_K783020
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagta ctgggcccaa gttcacttaa aaaggagatc
      241 aacaatgaaa gcaattttcg tactgaaaca tcttaatcat gctaaggagg ttttctaatg
      301 tactagagaa tgtccagatt agataaaagt aaagtgatta acagcgcatt agagctgctt
      361 aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct aggtgtagag
      421 cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt
      481 gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag ctggcaagat
      541 tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg cgatggagca
      601 aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga aaatcaatta
      661 gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact cagcgctgtg
      721 gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc taaagaagaa
      781 agggaaacac ctactactga tagtatgccg ccattattac gacaagctat cgaattattt
      841 gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat atgcggatta
      901 gaaaaacaac ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta cgctttagta
      961 gcttaataaa ggttactaga gaatgatggt gagcaagggc gaggagctgt tcaccggggt
     1021 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     1081 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     1141 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcaatgctt
     1201 cgcccgctac cccgaccaca tgaagctgca cgacttcttc aagtccgcca tgcccgaagg
     1261 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1321 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1381 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     1441 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     1501 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     1561 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc
     1621 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     1681 cggcatggac gagctgtaca agtaataaag gttactagag ccaggcatca aataaaacga
     1741 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1801 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//