LOCUS       BBa_K1401616            1876 bp    DNA     linear       22-Nov-2024
DEFINITION  pLac - BCD2 - tetR- GFP
ACCESSION   BBa_K1401616
VERSION     BBa_K1401616.1  
FEATURES             Location/Qualifiers
     misc_feature    1..200
                     /label=BBa_R0010
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_K783046
     RBS             213..296
                     /label=BCD2
     misc_feature    209..212
                     /label=MoClo Fusion Site B
     misc_feature    297..300
                     /label=MoClo Fusion Site C
     RBS             209..300
                     /label=BBa_K1114107
     CDS             309..1001
                     /label=BBa_K1401600
     misc_feature    1010..1739
                     /label=BBa_K1401602
     stem_loop       1759..1802
                     /label=stem_loop
     misc_feature    1748..1827
                     /label=BBa_B0010
     terminator      1748..1827
                     /label=BBa_B0010
     stem_loop       1843..1862
                     /label=T7 TE
     misc_feature    1869..1869
                     /label=stop
     polyA_site      1863..1876
                     /label=polya
     misc_feature    1836..1876
                     /label=BBa_B0012
     terminator      1836..1876
                     /label=BBa_B0012
     terminator      1748..1876
                     /label=BBa_K783020
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagta ctgggcccaa gttcacttaa aaaggagatc
      241 aacaatgaaa gcaattttcg tactgaaaca tcttaatcat gctaaggagg ttttctaatg
      301 tactagagaa tgatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg
      361 cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta
      421 gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc
      481 attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa
      541 gattttttac gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga
      601 gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa
      661 ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct
      721 gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa
      781 gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta
      841 tttgatcacc aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga
      901 ttagaaaaac aacttaaatg tgaaagtggg tccgctgcaa acgacgaaaa ctacgcttta
      961 gtagcttaat aacactgata gtgctagtgt agatcactct atactagagt ctagaatgcg
     1021 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
     1081 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
     1141 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
     1201 tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga
     1261 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
     1321 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
     1381 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
     1441 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
     1501 agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta
     1561 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
     1621 cacacaatct gccctttcga aagatcccaa cgaaaagaga gatcacatgg tccttcttga
     1681 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat aataaaggtt
     1741 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     1801 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     1861 cctttctgcg tttata
//