The parts contain the BioBricks - R0010_EB, BCD2_BC, C0040_CI, E0040m_ID and B0015_DF. All of these are MoClo variants i.e. they have MoClo flanking regions attached to each basic part. A new fusion site (I) was specifically designed to create the fusion protein (regulator + reporter).
All basic BioBricks used were transformed into MoClo variants by adding the following fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA; I = TCTAGA. These were then put together using the digestion-ligation MoClo reaction. E0040m has fixed illegal cut sites
This is the MoClo converted version of BBa_B0015, which is a double terminator consisting of BBa_B0010 and BBa_B0012. It has two MoClo fusion sites flanking it.
We had to design the proper MoClo fusion sites to flank this part.
This is a MoClo converted version of BBa_R0010
This is a MoClo converted version of BBa_R0010, which is an inverting regulator sensitive to LacI and CAP. It has two MoClo fusion sites flanking it.
Bicistronic Design RBS 2, MoClo Format with BC fusion sites
This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by Mutalik, et al.
This is the MoClo formatted part of a bicistronic design ribosomal binding site 1 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
Used for fusion proteins. More detail later
Fusion Sites (End sites) are very specific. A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; I = TCTA
This is a MoClo formatted E0040m_CD GFP gene.
This is a Level 0 MoClo part with flanking sites I on the 5' side and site D on the 3' side of the part.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1