BBa_K1401616
type: ComponentDefinition
persistent identity: BBa K1401616
display id: BBa_K1401616
version: 1
was generated by: iGEM to SBOL conversion
description: pLac - BCD2 - tetR- GFP
created: 2014-10-10T11:00:00Z
modified: 2015-05-08T01:10:16Z
mutable provenance: E. Coli
owned by: james
owned by: myers
mutable description: The parts contain the BioBricks - R0010_EB, BCD2_BC, C0040_CI, E0040m_ID and B0015_DF. All of these are MoClo variants i.e. they have MoClo flanking regions attached to each basic part. A new fusion site (I) was specifically designed to create the fusion protein (regulator + reporter).
discontinued: false
dominant: false
group u list: _1779_
m user id: 0
owner id: 23227
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: All basic BioBricks used were transformed into MoClo variants by adding the following fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA; I = TCTAGA. These were then put together using the digestion-ligation MoClo reaction. E0040m has fixed illegal cut sites
star: false
creator: Yash Agarwal
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K783020
component: BBa_K1401602
component: BBa_K1401600
component: BBa_K1114107
component: BBa_K783046
sequence annotation: BBa_K783046
sequence annotation: BBa_K1114107
sequence annotation: BBa_K1401600
sequence annotation: BBa_K1401602
sequence annotation: BBa_K783020
sequence: https://synbiohub.org/public/igem/BBa_K1401616_sequence/1

BBa_K783020
type: Component
persistent identity: component2417285
display id: component2417285
version: 1
top level: BBa_K1401616
definition: BBa_K783020
access: public

BBa_K1401602
type: Component
persistent identity: component2417278
display id: component2417278
version: 1
top level: BBa_K1401616
definition: BBa_K1401602
access: public

BBa_K1401600
type: Component
persistent identity: component2417277
display id: component2417277
version: 1
top level: BBa_K1401616
definition: BBa_K1401600
access: public

BBa_K1114107
type: Component
persistent identity: component2417276
display id: component2417276
version: 1
top level: BBa_K1401616
definition: BBa_K1114107
access: public

BBa_K783046
type: Component
persistent identity: component2417272
display id: component2417272
version: 1
top level: BBa_K1401616
definition: BBa_K783046
access: public

BBa_K783046
type: SequenceAnnotation
persistent identity: annotation2417272
display id: annotation2417272
version: 1
top level: BBa_K1401616
location: https://synbiohub.org/public/igem/BBa_K1401616/annotation2417272/range2417272/1
component: BBa_K783046

https://synbiohub.org/public/igem/BBa_K1401616/annotation2417272/range2417272/1
type: Range
persistent identity: range2417272
display id: range2417272
version: 1
top level: BBa_K1401616
direction: direction/reverse
start: 1
end: 200
orientation: inline

BBa_K1114107
type: SequenceAnnotation
persistent identity: annotation2417276
display id: annotation2417276
version: 1
top level: BBa_K1401616
location: https://synbiohub.org/public/igem/BBa_K1401616/annotation2417276/range2417276/1
component: BBa_K1114107

https://synbiohub.org/public/igem/BBa_K1401616/annotation2417276/range2417276/1
type: Range
persistent identity: range2417276
display id: range2417276
version: 1
top level: BBa_K1401616
direction: direction/reverse
start: 209
end: 300
orientation: inline

BBa_K1401600
type: SequenceAnnotation
persistent identity: annotation2417277
display id: annotation2417277
version: 1
top level: BBa_K1401616
location: https://synbiohub.org/public/igem/BBa_K1401616/annotation2417277/range2417277/1
component: BBa_K1401600

https://synbiohub.org/public/igem/BBa_K1401616/annotation2417277/range2417277/1
type: Range
persistent identity: range2417277
display id: range2417277
version: 1
top level: BBa_K1401616
direction: direction/reverse
start: 309
end: 1001
orientation: inline

BBa_K1401602
type: SequenceAnnotation
persistent identity: annotation2417278
display id: annotation2417278
version: 1
top level: BBa_K1401616
location: https://synbiohub.org/public/igem/BBa_K1401616/annotation2417278/range2417278/1
component: BBa_K1401602

https://synbiohub.org/public/igem/BBa_K1401616/annotation2417278/range2417278/1
type: Range
persistent identity: range2417278
display id: range2417278
version: 1
top level: BBa_K1401616
direction: direction/reverse
start: 1010
end: 1739
orientation: inline

BBa_K783020
type: SequenceAnnotation
persistent identity: annotation2417285
display id: annotation2417285
version: 1
top level: BBa_K1401616
location: https://synbiohub.org/public/igem/BBa_K1401616/annotation2417285/range2417285/1
component: BBa_K783020

https://synbiohub.org/public/igem/BBa_K1401616/annotation2417285/range2417285/1
type: Range
persistent identity: range2417285
display id: range2417285
version: 1
top level: BBa_K1401616
direction: direction/reverse
start: 1748
end: 1876
orientation: inline

BBa_K783020
type: ComponentDefinition
persistent identity: BBa K783020
display id: BBa_K783020
version: 1
was generated by: iGEM to SBOL conversion
description: MoClo converted version of BBa_B0015
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:20Z
mutable provenance: BBa_B0015
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the MoClo converted version of BBa_B0015, which is a double terminator consisting of BBa_B0010 and BBa_B0012. It has two MoClo fusion sites flanking it.
discontinued: false
dominant: false
group u list: _1038_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: We had to design the proper MoClo fusion sites to flank this part.
star: false
creator: Traci Haddock
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K783020_sequence/1

BBa_B0010
type: Component
persistent identity: component2214216
display id: component2214216
version: 1
top level: BBa_K783020
definition: BBa_B0010
access: public

BBa_B0012
type: Component
persistent identity: component2214218
display id: component2214218
version: 1
top level: BBa_K783020
definition: BBa_B0012
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2214216
display id: annotation2214216
version: 1
top level: BBa_K783020
location: https://synbiohub.org/public/igem/BBa_K783020/annotation2214216/range2214216/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K783020/annotation2214216/range2214216/1
type: Range
persistent identity: range2214216
display id: range2214216
version: 1
top level: BBa_K783020
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2214218
display id: annotation2214218
version: 1
top level: BBa_K783020
location: https://synbiohub.org/public/igem/BBa_K783020/annotation2214218/range2214218/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K783020/annotation2214218/range2214218/1
type: Range
persistent identity: range2214218
display id: range2214218
version: 1
top level: BBa_K783020
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K783046
type: ComponentDefinition
persistent identity: BBa K783046
display id: BBa_K783046
version: 1
was generated by: iGEM to SBOL conversion
description: This is a MoClo converted version of BBa_R0010
created: 2012-10-01T11:00:00Z
modified: 2015-05-08T01:13:20Z
mutable provenance: BBa_R0010
owned by: myers
owned by: james
part status: Released HQ 2013
mutable description: This is a MoClo converted version of BBa_R0010, which is an inverting regulator sensitive to LacI and CAP. It has two MoClo fusion sites flanking it.
discontinued: false
dominant: false
group u list: _1038_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: We had to design the proper MoClo fusion sites to flank this part.
star: false
creator: Traci Haddock
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K783046_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2214251
display id: annotation2214251
version: 1
top level: BBa_K783046
location: https://synbiohub.org/public/igem/BBa_K783046/annotation2214251/range2214251/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K783046/annotation2214251/range2214251/1
type: Range
persistent identity: range2214251
display id: range2214251
version: 1
top level: BBa_K783046
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2214250
display id: annotation2214250
version: 1
top level: BBa_K783046
location: https://synbiohub.org/public/igem/BBa_K783046/annotation2214250/range2214250/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K783046/annotation2214250/range2214250/1
type: Range
persistent identity: range2214250
display id: range2214250
version: 1
top level: BBa_K783046
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2214252
display id: annotation2214252
version: 1
top level: BBa_K783046
location: https://synbiohub.org/public/igem/BBa_K783046/annotation2214252/range2214252/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K783046/annotation2214252/range2214252/1
type: Range
persistent identity: range2214252
display id: range2214252
version: 1
top level: BBa_K783046
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2214253
display id: annotation2214253
version: 1
top level: BBa_K783046
location: https://synbiohub.org/public/igem/BBa_K783046/annotation2214253/range2214253/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K783046/annotation2214253/range2214253/1
type: Range
persistent identity: range2214253
display id: range2214253
version: 1
top level: BBa_K783046
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2214254
display id: annotation2214254
version: 1
top level: BBa_K783046
location: https://synbiohub.org/public/igem/BBa_K783046/annotation2214254/range2214254/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K783046/annotation2214254/range2214254/1
type: Range
persistent identity: range2214254
display id: range2214254
version: 1
top level: BBa_K783046
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2214255
display id: annotation2214255
version: 1
top level: BBa_K783046
location: https://synbiohub.org/public/igem/BBa_K783046/annotation2214255/range2214255/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K783046/annotation2214255/range2214255/1
type: Range
persistent identity: range2214255
display id: range2214255
version: 1
top level: BBa_K783046
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2214256
display id: annotation2214256
version: 1
top level: BBa_K783046
location: https://synbiohub.org/public/igem/BBa_K783046/annotation2214256/range2214256/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K783046/annotation2214256/range2214256/1
type: Range
persistent identity: range2214256
display id: range2214256
version: 1
top level: BBa_K783046
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_K1114107
type: ComponentDefinition
persistent identity: BBa K1114107
display id: BBa_K1114107
version: 1
was generated by: iGEM to SBOL conversion
description: Bicistronic Design RBS 2, MoClo Format with BC fusion sites
created: 2013-09-06T11:00:00Z
modified: 2015-05-08T01:09:11Z
mutable provenance: This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by Mutalik, et al.
owned by: james
owned by: myers
mutable description: This is the MoClo formatted part of a bicistronic design ribosomal binding site 1 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
discontinued: false
dominant: false
group u list: _1425_
m user id: 0
owner id: 8248
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This is the MoClo formatted part of a bicistronic design ribosomal binding site 1 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.
star: false
creator: Traci Haddock
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: MoClo Fusion Site B
sequence annotation: BCD2
sequence annotation: MoClo Fusion Site C
sequence: https://synbiohub.org/public/igem/BBa_K1114107_sequence/1

MoClo Fusion Site B
type: SequenceAnnotation
persistent identity: annotation2361727
display id: annotation2361727
version: 1
top level: BBa_K1114107
location: https://synbiohub.org/public/igem/BBa_K1114107/annotation2361727/range2361727/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1114107/annotation2361727/range2361727/1
type: Range
persistent identity: range2361727
display id: range2361727
version: 1
top level: BBa_K1114107
direction: direction/forward
start: 1
end: 4
orientation: inline

BCD2
type: SequenceAnnotation
persistent identity: annotation2361724
display id: annotation2361724
version: 1
top level: BBa_K1114107
location: https://synbiohub.org/public/igem/BBa_K1114107/annotation2361724/range2361724/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1114107/annotation2361724/range2361724/1
type: Range
persistent identity: range2361724
display id: range2361724
version: 1
top level: BBa_K1114107
direction: direction/forward
start: 5
end: 88
orientation: inline

MoClo Fusion Site C
type: SequenceAnnotation
persistent identity: annotation2361728
display id: annotation2361728
version: 1
top level: BBa_K1114107
location: https://synbiohub.org/public/igem/BBa_K1114107/annotation2361728/range2361728/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1114107/annotation2361728/range2361728/1
type: Range
persistent identity: range2361728
display id: range2361728
version: 1
top level: BBa_K1114107
direction: direction/forward
start: 89
end: 92
orientation: inline

BBa_K1401600
type: ComponentDefinition
persistent identity: BBa K1401600
display id: BBa_K1401600
version: 1
was generated by: iGEM to SBOL conversion
description: C0040_CI
created: 2014-07-20T11:00:00Z
modified: 2015-05-08T01:10:16Z
mutable provenance: iGEM distribution kit 2014
owned by: james
owned by: myers
mutable description: Used for fusion proteins. More detail later
discontinued: false
dominant: false
experience: experience/None
group u list: _1779_
m user id: 0
owner id: 23227
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Fusion Sites (End sites) are very specific. A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; I = TCTA
star: false
creator: Yash Agarwal
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1401600_sequence/1

BBa_K1401602
type: ComponentDefinition
persistent identity: BBa K1401602
display id: BBa_K1401602
version: 1
was generated by: iGEM to SBOL conversion
description: E0040m_ID
created: 2014-08-10T11:00:00Z
modified: 2015-05-08T01:10:16Z
mutable provenance: Source entered later.
owned by: james
owned by: myers
mutable description: This is a MoClo formatted E0040m_CD GFP gene.
discontinued: false
dominant: false
experience: experience/None
group u list: _1779_
m user id: 0
owner id: 23227
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This is a Level 0 MoClo part with flanking sites I on the 5' side and site D on the 3' side of the part.
star: false
creator: Yash Agarwal
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1401602_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1401616_sequence/1
type: Sequence
persistent identity: BBa K1401616 sequence
display id: BBa_K1401616_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtactgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgtactagagaatgatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactctatactagagtctagaatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagatcacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataaaggttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K783020_sequence/1
type: Sequence
persistent identity: BBa K783020 sequence
display id: BBa_K783020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K783046_sequence/1
type: Sequence
persistent identity: BBa K783046 sequence
display id: BBa_K783046_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1114107_sequence/1
type: Sequence
persistent identity: BBa K1114107 sequence
display id: BBa_K1114107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1401600_sequence/1
type: Sequence
persistent identity: BBa K1401600 sequence
display id: BBa_K1401600_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aatgatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactcta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1401602_sequence/1
type: Sequence
persistent identity: BBa K1401602 sequence
display id: BBa_K1401602_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctagaatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagatcacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataaaggt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z