BBa_K1402005 1 BBa_K1402005 RBS-AtFatA-Terminator 2014-09-28T11:00:00Z 2015-05-08T01:10:16Z Arabidopsis thaliana Acyl-acyl carrier protein (ACP) thioesterase catalyzes the hydrolysis of acyl-ACP to fatty acids and ACP this reaction removes the coupling between fatty acid biosynthesis and phospholipid biosyntheis,which is a key to obtain free fatty acids. This thioesterase AtFatA can produce fatty acids with 16~18 carbon chain length. In previous researches, thioesterase gene AtFatA can be expressed in E.coli BL21(DE3) and enhance the synthesis of free fatty acids. This thioesterase can express itself with a T7 promoter. The RBS is in front of the AtFatA coding sequence and T7 terminator is after AtFatA. You can design your own promoter for this part, but use this part in E.coli BL21(DE3) only or you???d better delete the T7 terminator. false false _1780_ 0 16730 9 In stock false no false Chen Xi BBa_K1402005_sequence 1 aaagaggagaaaaatactagaagagattcattcacagtgttgaaattaaaaaaccgaaactttctcgtttcttcttcttcttctccaattttcaaaattcgaaaagatgttgaagctttcgtgtaatgtgactgattctaagttacagagaagcttactcttcttctcccattcatatcgatctgatccggtgaatttcatccgtcggagaattgtctcttgttctcagacgaagaagacaggtttggttcctttgcgtgctgttgtatctgctgatcaaggaagtgtggttcaaggtttggctactctcgcggatcagctccgattaggtagtttgactgaagatggtttatcttataaagagaagtttgttgttagatcttacgaagtgggtagtaacaaaaccgctactgttgaaaccattgctaatcttttacaggaggtgggatgtaatcatgcacaaagtgttggtttttcgactgatgggtttgcaacaacaactactatgaggaagttgcatctcatttgggttactgcgagaatgcatatcgagatctataagtaccctgcttggggtgatgtggttgagatagagacttggtgtcagagtgaaggaaggattgggacaaggcgtgattggattcttaaggattctgtcactggtgaagtcactggccgtgctacaagcaagtgggtgatgatgaaccaagacacgagacggcttcagaaagtttctgatgatgttcgggacgagtacttggtcttctgtcctcaagaaccgaggttagcatttccggaagagaataacagaagcttgaagaaaatcccgaaactcgaagatccggctcagtattcaatgattgggcttaagcctagacgagctgatctcgacatgaaccagcatgtcaataatgtcacctatattggatgggttctcgagagcataccacaagaaattgtagacacgcacgagcttcaggtcataactctggattatagaagagaatgtcaacaagacgatgtggtggattcactcaccaccaccacctctgaaattggtggaaccaatggctctgccacgtctggcacacagggccacaacgatagccagttcttgcacctcctgaggttgtctggagatggtcaggagatcaaccgcgggacaactctgtggagaaagaagccttcaagttaagaaatagacaatgtctttagccattttgttctcaagtttccatcatctcaatgaagatttcgcttcacgagtctgagcaggtctccatttttttctctttcaagtttgggttagactagagggaactggattgttggagtattaatctttgttgaatttcattatgtttgttcatgtttttgtacaaattttggggatttagccaaacccatatcgtctttggtaaaaaaaaaactgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z