BBa_K1402006
1
BBa_K1402006
T7-RBS-AtFatA-Terminator
2014-09-28T11:00:00Z
2015-05-08T01:10:16Z
Arabidopsis thaliana
Acyl-acyl carrier protein (ACP) thioesterase catalyzes the hydrolysis of acyl-ACP to fatty acids and ACP this reaction removes the coupling between fatty acid biosynthesis and phospholipid biosyntheis,which is a key to obtain free fatty acids. This thioesterase AtFatA can produce fatty acids with 16~18 carbon chain length. In previous researches, thioesterase gene AtFatA can be expressed in E.coli BL21(DE3) and enhance the synthesis of free fatty acids.
This thioesterase can express itself with a T7 promoter.
The RBS and T7 promoter are in front of the AtFatA coding sequence and T7 terminator is after AtFatA. You can only use this part in E.coli BL21(DE3) or choose other parts we offer in other strains.
false
false
_1780_
0
16730
9
It's complicated
false
no
false
Chen Xi
BBa_K1402006_sequence
1
gcggccgcttctagataatacgactcactataggaaagaggagaaaaatactagaagagattcattcacagtgttgaaattaaaaaaccgaaactttctcgtttcttcttcttcttctccaattttcaaaattcgaaaagatgttgaagctttcgtgtaatgtgactgattctaagttacagagaagcttactcttcttctcccattcatatcgatctgatccggtgaatttcatccgtcggagaattgtctcttgttctcagacgaagaagacaggtttggttcctttgcgtgctgttgtatctgctgatcaaggaagtgtggttcaaggtttggctactctcgcggatcagctccgattaggtagtttgactgaagatggtttatcttataaagagaagtttgttgttagatcttacgaagtgggtagtaacaaaaccgctactgttgaaaccattgctaatcttttacaggaggtgggatgtaatcatgcacaaagtgttggtttttcgactgatgggtttgcaacaacaactactatgaggaagttgcatctcatttgggttactgcgagaatgcatatcgagatctataagtaccctgcttggggtgatgtggttgagatagagacttggtgtcagagtgaaggaaggattgggacaaggcgtgattggattcttaaggattctgtcactggtgaagtcactggccgtgctacaagcaagtgggtgatgatgaaccaagacacgagacggcttcagaaagtttctgatgatgttcgggacgagtacttggtcttctgtcctcaagaaccgaggttagcatttccggaagagaataacagaagcttgaagaaaatcccgaaactcgaagatccggctcagtattcaatgattgggcttaagcctagacgagctgatctcgacatgaaccagcatgtcaataatgtcacctatattggatgggttctcgagagcataccacaagaaattgtagacacgcacgagcttcaggtcataactctggattatagaagagaatgtcaacaagacgatgtggtggattcactcaccaccaccacctctgaaattggtggaaccaatggctctgccacgtctggcacacagggccacaacgatagccagttcttgcacctcctgaggttgtctggagatggtcaggagatcaaccgcgggacaactctgtggagaaagaagccttcaagttaagaaatagacaatgtctttagccattttgttctcaagtttccatcatctcaatgaagatttcgcttcacgagtctgagcaggtctccatttttttctctttcaagtttgggttagactagagggaactggattgttggagtattaatctttgttgaatttcattatgtttgttcatgtttttgtacaaattttggggatttagccaaacccatatcgtctttggtaaaaaaaaaactgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z