BBa_K1402005 1 BBa_K1402005 RBS-AtFatA-Terminator 2014-09-28T11:00:00Z 2015-05-08T01:10:16Z Arabidopsis thaliana Acyl-acyl carrier protein (ACP) thioesterase catalyzes the hydrolysis of acyl-ACP to fatty acids and ACP this reaction removes the coupling between fatty acid biosynthesis and phospholipid biosyntheis,which is a key to obtain free fatty acids. This thioesterase AtFatA can produce fatty acids with 16~18 carbon chain length. In previous researches, thioesterase gene AtFatA can be expressed in E.coli BL21(DE3) and enhance the synthesis of free fatty acids. This thioesterase can express itself with a T7 promoter. The RBS is in front of the AtFatA coding sequence and T7 terminator is after AtFatA. You can design your own promoter for this part, but use this part in E.coli BL21(DE3) only or you???d better delete the T7 terminator. false false _1780_ 0 16730 9 In stock false no false Chen Xi BBa_K1402010 1 BBa_K1402010 Lac Promoter 2014-09-28T11:00:00Z 2015-05-08T01:10:16Z E.coli This part is an inverting regulator sensitive to LacI (BBa_C0010, BBa_C0012, etc.) The T7-lac promoter is a hybrid regulatory region consisting of the T7 promoter of T7 phage next to LacO. The hybrid design allows for strong promotion that can nevertheless be:  repressed by LacI  induced by lac or IPTG, showing a high expression level.  CAP protein will not influence the expression. Strong promoter. Only E.Coli BL21(DE3) can use this part. false false _1780_ 0 16730 9 In stock false no false Chen Xi BBa_K1402007 1 BBa_K1402007 Lac-RBS-AtFatA-Treminator 2014-09-28T11:00:00Z 2015-05-08T01:10:16Z Arabidopsis thaliana Acyl-acyl carrier protein (ACP) thioesterase catalyzes the hydrolysis of acyl-ACP to fatty acids and ACP this reaction removes the coupling between fatty acid biosynthesis and phospholipid biosyntheis,which is a key to obtain free fatty acids.In before study, thioesterase gene AtFatA can express in E.coli BL21(DE3),and increase free fatty acids. This thioesterase can be expressed in the presence of lactose or IPTG. The RBS and lac promoter are in front of the AtFatA coding sequence and T7 terminator is after AtFatA. We can activate the expression of AtFatA by adding lactose in the culture in the absence of glucose.You can only use this part in E.coli BL21(DE3) because the lac promoter we use is the hybrid of lac promoter and T7 promoter. false false _1780_ 0 16730 9 It's complicated false no false Chen Xi component2389282 1 BBa_K1402010 component2389281 1 BBa_K1402005 annotation2389282 1 BBa_K1402010 range2389282 1 1567 1645 annotation2389281 1 BBa_K1402005 range2389281 1 1 1558 BBa_K1402005_sequence 1 aaagaggagaaaaatactagaagagattcattcacagtgttgaaattaaaaaaccgaaactttctcgtttcttcttcttcttctccaattttcaaaattcgaaaagatgttgaagctttcgtgtaatgtgactgattctaagttacagagaagcttactcttcttctcccattcatatcgatctgatccggtgaatttcatccgtcggagaattgtctcttgttctcagacgaagaagacaggtttggttcctttgcgtgctgttgtatctgctgatcaaggaagtgtggttcaaggtttggctactctcgcggatcagctccgattaggtagtttgactgaagatggtttatcttataaagagaagtttgttgttagatcttacgaagtgggtagtaacaaaaccgctactgttgaaaccattgctaatcttttacaggaggtgggatgtaatcatgcacaaagtgttggtttttcgactgatgggtttgcaacaacaactactatgaggaagttgcatctcatttgggttactgcgagaatgcatatcgagatctataagtaccctgcttggggtgatgtggttgagatagagacttggtgtcagagtgaaggaaggattgggacaaggcgtgattggattcttaaggattctgtcactggtgaagtcactggccgtgctacaagcaagtgggtgatgatgaaccaagacacgagacggcttcagaaagtttctgatgatgttcgggacgagtacttggtcttctgtcctcaagaaccgaggttagcatttccggaagagaataacagaagcttgaagaaaatcccgaaactcgaagatccggctcagtattcaatgattgggcttaagcctagacgagctgatctcgacatgaaccagcatgtcaataatgtcacctatattggatgggttctcgagagcataccacaagaaattgtagacacgcacgagcttcaggtcataactctggattatagaagagaatgtcaacaagacgatgtggtggattcactcaccaccaccacctctgaaattggtggaaccaatggctctgccacgtctggcacacagggccacaacgatagccagttcttgcacctcctgaggttgtctggagatggtcaggagatcaaccgcgggacaactctgtggagaaagaagccttcaagttaagaaatagacaatgtctttagccattttgttctcaagtttccatcatctcaatgaagatttcgcttcacgagtctgagcaggtctccatttttttctctttcaagtttgggttagactagagggaactggattgttggagtattaatctttgttgaatttcattatgtttgttcatgtttttgtacaaattttggggatttagccaaacccatatcgtctttggtaaaaaaaaaactgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat BBa_K1402007_sequence 1 aaagaggagaaaaatactagaagagattcattcacagtgttgaaattaaaaaaccgaaactttctcgtttcttcttcttcttctccaattttcaaaattcgaaaagatgttgaagctttcgtgtaatgtgactgattctaagttacagagaagcttactcttcttctcccattcatatcgatctgatccggtgaatttcatccgtcggagaattgtctcttgttctcagacgaagaagacaggtttggttcctttgcgtgctgttgtatctgctgatcaaggaagtgtggttcaaggtttggctactctcgcggatcagctccgattaggtagtttgactgaagatggtttatcttataaagagaagtttgttgttagatcttacgaagtgggtagtaacaaaaccgctactgttgaaaccattgctaatcttttacaggaggtgggatgtaatcatgcacaaagtgttggtttttcgactgatgggtttgcaacaacaactactatgaggaagttgcatctcatttgggttactgcgagaatgcatatcgagatctataagtaccctgcttggggtgatgtggttgagatagagacttggtgtcagagtgaaggaaggattgggacaaggcgtgattggattcttaaggattctgtcactggtgaagtcactggccgtgctacaagcaagtgggtgatgatgaaccaagacacgagacggcttcagaaagtttctgatgatgttcgggacgagtacttggtcttctgtcctcaagaaccgaggttagcatttccggaagagaataacagaagcttgaagaaaatcccgaaactcgaagatccggctcagtattcaatgattgggcttaagcctagacgagctgatctcgacatgaaccagcatgtcaataatgtcacctatattggatgggttctcgagagcataccacaagaaattgtagacacgcacgagcttcaggtcataactctggattatagaagagaatgtcaacaagacgatgtggtggattcactcaccaccaccacctctgaaattggtggaaccaatggctctgccacgtctggcacacagggccacaacgatagccagttcttgcacctcctgaggttgtctggagatggtcaggagatcaaccgcgggacaactctgtggagaaagaagccttcaagttaagaaatagacaatgtctttagccattttgttctcaagtttccatcatctcaatgaagatttcgcttcacgagtctgagcaggtctccatttttttctctttcaagtttgggttagactagagggaactggattgttggagtattaatctttgttgaatttcattatgtttgttcatgtttttgtacaaattttggggatttagccaaacccatatcgtctttggtaaaaaaaaaactgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggattactagaggaaattaatacgactcactataggggaattgtgagcggataacaattccccactagaaataattttgtttaactttaag BBa_K1402010_sequence 1 gaaattaatacgactcactataggggaattgtgagcggataacaattccccactagaaataattttgtttaactttaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z