BBa_K1403002 1 AgaA AgaA expression (3M2H-> 3H3MH) 2014-10-05T11:00:00Z 2015-05-08T01:10:17Z agaA sequence codon optimized for E coli 12 (IDT online tool) agaA is an enzyme present in Corynebacterium striatum that is the main responsible for body odor. It metabolizes 3-methyl-2-hexenoic acid into 3-hydroxy-3-methylhexanoic acid, that is a sulphure volatile compound. Name: BBa_K1403002 Description: Biobrick submission of agaA expression construct Resistances : Cm Backbone : pSB1C3 Parts: Pcons - RBS - agaA - his tag Design date: 11/June/2014 This part contains: 1) Plasmid pSB1C3: High copy BioBrick assembly plasmid. Constitutive expression. To use in E coli 2) agaA construct: - Promoter BBa_J23108 from the Anderson's promoter collection - RBS from the RBS Calculator of Salis lab specific for E coli - BioBrick Prefix + Scar from the iGEM Parts webpage - agaA sequence codon optimized for E coli 12 (IDT online tool). We checked with Geneious that there were no restriction sites for EcoRI, SpeI, XbaI and PstI. - Histidine tag: 6 Histidines in C-terminal were added -Stop codon -BioBrick scar + BioBrick suffix from the iGEM Parts webpage false false _1781_ 0 22924 9 It's complicated false Codon optimization. Check no restriction sites for EcoRI, SpeI, XbaI and PstI. false Cristina Garcia-Timermans BBa_K1403002_sequence 1 atggcgcaagaaaaccttcagaaaatcgtggatagtttagaatcgtcacgcgcagaacgtgaagaactgtacaaatggtttcatcagcaccctgaaatgagcatgcaggagcatgaaactagcaaacgtatcgcagaggaattggagaaactgggcctcgaaccacagaatattggtgtgaccggtcaggtagcagttatcaaaaatggtgaaggaccgtcggttgcgtttcgtgccgactttgacgcgcttcctattacggaaaacacgggcctggattactctgcggatccggaactgggtatgatgcatgcctgtggccatgacctgcatactaccgccctcctgggcgcagtgcgtgccctggttgaaaataaggatctgtggagtggcacgtttattgcggtccaccaaccgggagaggagggcggtggcggggcccgtcatatggtcgacgacggtctggcggaaaagattgcggctccggatgtctgctttgctcaacatgtgtttaatgaggatccggcgtttggttatgtttttacgcccggtcgtttcctgacagcagcatccaattggcgtatccacattcatggggaaggtgggcacggtagccgtccacatttaactaaagatccgattgtggtcgcggcgtctattattaccaaattgcagaccattgttagccgcgaagtagatccgaatgaggtggcagttgtgacggtgggttccatcgaaggtggcaagagtaccaacagtattccatatacagtaaccctgggagtaaatacccgcgccagcaatgatgaactgtcggagtatgtccagaacgcgattaaacgtattgtcattgcagaatgtcaggcggcaggcattgaacaggaacctgaatttgagtatctggattccgttccggcggttatcaacgatgaagatttgaccgaacagcttatggcgcaatttcgcgagtttttcggcgaagatcaggcggtcgaaatcccgccgttatcaggatcagaagattatccgtttatcccaaacgcgtggggcgtaccgtctgttatgtggggctggtcgggttttgccgcgggtagcgacgcgccggggaaccataccgacaaattcgccccggaattgccggatgccttggaacgtgggacccaagccattctggttgctgcggccccttggttgatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z