LOCUS       BBa_K1403012            1782 bp    DNA     linear       22-Nov-2024
DEFINITION  Alcohol acetyltransferase I (ATF1) expression cassette
ACCESSION   BBa_K1403012
VERSION     BBa_K1403012.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=Promoter
     misc_feature    36..43
                     /label=Scar
     RBS             44..58
                     /label=RBS BBa_B0030
     misc_feature    59..64
                     /label=Scar
     CDS             65..1639
                     /label=ATF1
     misc_feature    1640..1642
                     /label=stop
     misc_feature    1643..1645
                     /label=stop
     misc_feature    1646..1653
                     /label=Scar
     misc_feature    1654..1733
                     /label=BBa_B0010
     stem_loop       1665..1708
                     /label=stem_loop
     misc_feature    1734..1741
                     /label=Scar
     misc_feature    1742..1782
                     /label=BBa_B0012
     stem_loop       1749..1768
                     /label=T7 TE
     polyA_site      1769..1782
                     /label=polya
ORIGIN
        1 ctgacagcta gctcagtcct aggtataatg ctagctacta gagattaaag aggagaaata
       61 ctagatgaat gaaatcgatg agaaaaatca ggcccccgtg caacaagaat gcctgaaaga
      121 gatgattcag aatgggcatg ctcggcgtat gggatctgtt gaagatctgt atgttgctct
      181 caacagacaa aacttatatc gaaacttctg cacatatgga gaattgagtg attactgtac
      241 tagggatcag ctcacattag ctttgaggga aatctgcctg aaaaatccaa ctcttttaca
      301 tattgttcta ccaacaagat ggccaaatca tgaaaattat tatcgcagtt ccgaatacta
      361 ttcacggcca catccagtgc atgattatat ttcagtatta caagaattga aactgagtgg
      421 tgtggttctc aatgaacaac ctgagtacag tgcagtaatg aagcaaatat tagaagagtt
      481 caaaaatagt aagggttcct atactgcaaa aatttttaaa cttactacca ctttgactat
      541 tccttacttt ggaccaacag gaccgagttg gcggctaatt tgtcttccag aagagcacac
      601 agaaaagtgg aaaaaattta tctttgtatc taatcattgc atgtctgatg gtcggtcttc
      661 gatccacttt tttcatgatt taagagacga attaaataat attaaaactc caccaaaaaa
      721 attagattac attttcaagt acgaggagga ttaccaatta ttgaggaaac ttccagaacc
      781 gatcgaaaag gtgatagact ttagaccacc gtacttgttt attccgaagt cacttctttc
      841 gggtttcatc tacaatcatt tgagattttc ttcaaaaggt gtctgtatga gaatggatga
      901 tgtggaaaaa accgatgatg ttgtcaccga gatcatcaat atttcaccaa cagaatttca
      961 agcgattaaa gcaaatatta aatcaaatat ccaaggtaag tgtactatca ctccgttttt
     1021 acatgtttgt tggtttgtat ctcttcataa atggggtaaa tttttcaaac cattgaactt
     1081 cgaatggctt acggatattt ttatccccgc agattgccgc tcacaactac cagatgatga
     1141 tgaaatgaga cagatgtaca gatatggcgc taacgttgga tttattgact tcaccccctg
     1201 gataagcgaa tctgacatga atgataacaa agaaaatttt tggccactta ttgagcacta
     1261 ccatgaagta atttcggaag ctttaagaaa taaaaagcat ctccatggct tagggttcaa
     1321 tatacaaggc ttcgttcaaa aatatgtgaa cattgacaag gtaatgtgcg atcgtgccat
     1381 cgggaaaaga cgcggaggta cattgttaag caatgtaggt ctgtttaatc agttagagga
     1441 gcccgatgcc aaatattcta tatgcgattt ggcatttggc caatttcaag gatcctggca
     1501 ccaagcattt tccttgggtg tttgttcgac taatgtaaag gggatgaata ttgttgttgc
     1561 ttcaacaaag aatgttgttg gtagtcaaga atctctcgaa gagctttgct ccatttacaa
     1621 agctctcctt ttaggccctt aataatacta gagccaggca tcaaataaaa cgaaaggctc
     1681 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1741 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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