LOCUS BBa_K1403019 2102 bp DNA linear 29-Nov-2024 DEFINITION Acetohydroxybutanoate synthase /acetolactate synthase (ilvB/N) expression cassette ACCESSION BBa_K1403019 VERSION BBa_K1403019.1 FEATURES Location/Qualifiers promoter 1..17 /label=T7 Promoter primer_bind 3..20 /label=Sequencing (Rev) misc_feature 22..42 /label=lacO RBS 75..80 /label=RBS misc_feature 87..90 /label=Scar primer_bind 97..114 /label=Extraction (Fwd) CDS 109..399 /label=ilvN CDS 403..2091 /label=ilvB primer_bind 2081..2102 /label=Extraction (Rev) ORIGIN 1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt 61 tgtttaactt taagaaggag atataccatg ggttcaagcc ttgagcggtt actgaaaaaa 121 caccgcgatc ttgttaaaca tcgtcggatc ggactgatta cgctgcactt tcacgacatc 181 ttccagctta tcgatttggc ttatcatctg ctccagacgc tggtcgtcat tgaccagtag 241 ccagatatgg cttttgtcgc tgtcctgaat cggcagacaa agaatgcctt caacgttaaa 301 agcgcggcgg gcaaaaaggc cacaaacgtg ggtcattacg cccggatggt tgcgaacggt 361 gagctccaga attacgttgt catgagttgt gttttgcatg gcttattccc ccaccatttc 421 agtattcgcc gcacctggcg gcaccatcgg gtaaactttt tcttcggcat caatgcgcac 481 atggatcagc gccgggccag ggcgattgat gatttcctgc aatgaagcct gcggatcggc 541 ttcgttattc aaatcacagg tttcgaggcc gaatccggcg gcaatctgca taaagttgat 601 tttgcccgga taggtggcgg caaaaacgcc ttgctcgtag aacagactct gttgctgatg 661 caccagcccc agcgcttcgt tgttcatcag aatgattttg acatccagct gattttcact 721 ggcggtcgcc atctcctgaa tattcatcat caggctgccg tcgccggaga aacacaacac 781 tttgcgatcc gggttcgcca gcgcagcgcc aatcgccgca ggcaggccaa aacccatcgt 841 gcccagccca ccggaggtca gccactggcg tgggcgattg agcggataag cttgcgcggt 901 ccacatctga tgctgaccaa cgtcggtggt gataattgca ttgtcatcga cacaggcggc 961 aacggcgttg atcaggccgt aatggcttaa cggatcgcac gctttcggga ttggacacgg 1021 aaactcacgc tgcaaatccg ctaccaactg gtgccactct gcacgcggtt gcgcttccac 1081 cagcgggatc aactgcgcca gcacgtcatc aacatccgcc tgaatcgcca cgtgcggctg 1141 cttgatttta cccagctctg cacggtcgat atcgacatga atgattttgg cattcggaca 1201 gaactgctcg gttttgccaa tcgcccggtc atcaaaacgc gcaccgagca ctatcaacaa 1261 atccgcctcc tgcaaaatat agttggtgct gcgcacgccg tgcatcccca gcatacccag 1321 cgacaacgga tgcgcttttg gcaacatgcc cagcgccatt aaagtcatgg tggtaggcag 1381 ttgcgctttc tccgccagtt cacgcacccg tgcgggcgca ttgatcacac cgccgcccag 1441 ataaagcacc gggcgtttgg cagcgttaat catcgccgct gcgtcacgaa tgctttcttc 1501 gctaaaggcg ggggcggcgg ctttttctgc catagcgggc tgtgtttcaa tctcaaaaac 1561 tgccgtttgc acatccttag gaatgtctat ccacaccggg cctgggcggc ctgattgcgc 1621 aatgcggaag gcatcgctca tgacctgcgg gagttcttcg atatgtctga ccagatagtt 1681 gtgtttggtg atggggatag agatgccgta ggtgtccact tcctggaagg cgtcggtgcc 1741 gatcatcgag gcgggaacct gaccagtgat gcaaatcagc gggatggagt ccagccgcgc 1801 atcggcaatg gcggtcacca ggttagtcgc acccggtccg ctacaggcca tacagaccgc 1861 cggtttaccg tcggtgcgcg ccattccctg agcgataaag cccgcgccct gttcatgacg 1921 ggccagaata tggcggattt gcgtgctttg gcttaaggca tcgtaaacag gcaggataga 1981 accgcccgga atgcctgtca caatcttaat gccctgctgt tccaggaaat gaacgataaa 2041 ttctgcgccg gtaaagcgct tacgcgtcga tgttgtgccc gaacttgcca tgctccagtc 2101 ct //