LOCUS BBa_K1404001 4437 bp DNA linear 28-Jun-2024 DEFINITION Ptac-NiCoTB-rcn-csgBAEFG, turns E. coli into a functional Ni and Co biofilter ACCESSION BBa_K1404001 VERSION BBa_K1404001.1 FEATURES Location/Qualifiers promoter 1..76 /label=Ptac CDS 96..1212 /label=NiCoT RBS 83..95 /label=RBS Paris 2009 misc_feature 1212..1306 /label=B0014 terminator misc_feature 1..1306 /label=BBa_K1404002 CDS 1322..1594 /label=rcnR promoter 1709..1714 /label=-10rcnR promoter 1736..1741 /label=-35rcnR CDS 1715..1749 /label=rcnA RBS 1756..1768 /label=perfect RBS1 misc_feature 1483..1489 /label=Mut1 CDS 1771..2225 /label=csgB CDS 2266..2721 /label=csgA misc_feature 2636..2641 /label=Mut2 RBS 2729..2741 /label=perfect RBS2 CDS 2745..3134 /label=csgE misc_feature 3126..3131 /label=Mut3 CDS 3159..3575 /label=csgF CDS 3602..4435 /label=csgG misc_feature 1315..4437 /label=BBa_K540000 ORIGIN 1 aactgttaat tagtaggccg agcatattac acaccttaac actcgcctat tgttaaagtg 61 tgtcctttgt ctggtactag agaaggaggt atataatggg tggtgcggcg gcgcgcctgc 121 gccgccatcc gagctttgtt tatagcagca ttgcggaacc gattatgcat ctgaccaaaa 181 gcctgccgag cctgagcctg cgccgccgca ttggtgcgct gtttgcgggt ctgattgcgg 241 cgaacattgg tgtttgggtt tgggcgttta gcctgtttca tgcgcagccg ctgatgctgg 301 gtaccgcggt tctggcgtgg ggtctgggtc tgcgccatgc ggttgatgcg gatcatattg 361 cggcgattga taacgttacc cgcaaactga tgcaggatgg tcagcgcccg gttagcgttg 421 gtttttggtt tgcgattggt catagcggta ttattgcgat tgcgagcatt attattgcgg 481 ttaccgcgag cgcgctgagc cagtttggtg cgtttaaaga aattggtggt gttattgcga 541 ccgttattag cgcgctgttt ctgtttacca ttgcgggtat gaacctggtt attctgcgca 601 gcgtttggcg cacctttgcg catgttcgcg cgggtggtag ctatgcggaa gatgatctgg 661 atctgctgct gggtggtcgc ggtctgctga gccgcctgtt tcgcccgatg tttcgcctgg 721 ttaacaaaag ctggcatatg gcgccgctgg gttttctgtt tggtctgggt tttgataccg 781 cgaccgaagt tgcgattctg ggtatgagcg cgagccaggc ggcggatggt ctgagcattg 841 gtaccattct ggttctgccg gcgctgtttg cggttggtat ggcgctgatt gataccgcgg 901 atggtgttgt tatgctgggt gcgtatgaat gggcgtttgt taaaccgatt cgcaaactgt 961 attataacat taccattacc ctgattagcg cgattgttgc gattgttatt ggtggtattg 1021 aaaccctggc gctgattggt gataaactgg cgctgaccgg tgcgccgtgg cgcattgcga 1081 ttgaactggg tgaaaacttt aacggtctgg gttttgcgat tattggtctg tttgtttttt 1141 gctggctggc gagctatgcg atttatcgct ggaaacgctt tgatgaaatt gaagttagcg 1201 ttggtgcgtg atcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag 1261 agagaatata aaaagccaga ttattaatcc ggctttttta ttattttact agagttatta 1321 ttatttgata tatgaatcca gcaccttcag aacgacatcc agatcttctt cacgttttag 1381 ctcatccccc tggtgaacga tgtgttccgt cagatgacct ttaatcactt cccgcatcag 1441 accgtttacc gcgccacgga tagcagcaat ctgttgtaaa actgctgcgc attcgtgcgg 1501 ctcgtcgagc attttcttga gcgccacgac ctggccctga atcttactgg cacgcgcttt 1561 cagtttctgt ttatcacgga ttgtatgaga catggcaaca cctggttaac aagaatatga 1621 aaaatcatag cactattaat ctactggggg gtagtatcag gtactggggg ggagtagaat 1681 cagattgccg aattaatact aagaattatt atcatgaccg aatttacaac tcttcttcag 1741 caaggaaata ctagaaggag gtatataatg taaaaacaaa ttgttattta tgatgttaac 1801 aatactgggt gcgcctggga ttgcagccgc agcaggttat gatttagcta attcagaata 1861 taacttcgcg gtaaatgaat tgagtaagtc ttcatttaat caggcagcca taattggtca 1921 agctgggact aataatagtg ctcagttacg gcagggaggc tcaaaacttt tggcggttgt 1981 tgcgcaagaa ggtagtagca accgggcaaa gattgaccag acaggagatt ataaccttgc 2041 atatattgat caggcgggca gtgccaacga tgccagtatt tcgcaaggtg cttatggtaa 2101 tactgcgatg attatccaga aaggttctgg taataaagca aatattacac agtatggtac 2161 tcaaaaaacg gcaattgtag tgcagagaca gtcgcaaatg gctattcgcg tgacacaacg 2221 ttaatttcca ttcgactttt aaatcaatcc gatgggggtt ttacatgaaa cttttaaaag 2281 tagcagcaat tgcagcaatc gtattctccg gtagcgctct ggcaggtgtt gttcctcagt 2341 acggcggcgg cggtaaccac ggtggtggcg gtaataatag cggcccaaat tctgagctga 2401 acatttacca gtacggtggc ggtaactctg cacttgctct gcaaactgat gcccgtaact 2461 ctgacttgac tattacccag catggcggcg gtaatggtgc agatgttggt cagggctcag 2521 atgacagctc aatcgatctg acccaacgtg gcttcggtaa cagcgctact cttgatcagt 2581 ggaacggcaa aaattctgaa atgacggtta aacagttcgg tggtggcaac ggtgcagcag 2641 ttgaccagac tgcatctaac tcctccgtca acgtgactca ggttggcttt ggtaacaacg 2701 cgaccgctca tcagtactaa ctactagaag gaggtatata atgatgaaac gttatttacg 2761 ctggattgtg gcggcagaat ttctgttcgc cgcagggaat cttcacgccg ttgaggtaga 2821 agtcccggga ttgctaactg accatactgt ttcatctatt ggccatgatt tttaccgagc 2881 ctttagtgat aaatgggaaa gtgactatac gggtaactta acgattaatg aaaggcccag 2941 tgcacgatgg ggaagctgga tcactataac ggtcaatcag gacgttattt tccagacttt 3001 tttatttccg ttgaaaagag acttcgagaa aactgtcgtc tttgcactga ttcaaactga 3061 agaagcacta aatcgtcgcc agataaatca ggcgttatta agtacgggcg atttggcgca 3121 tgatgaattt taaataaaaa attgttcgga ggctgcaatg cgtgtcaaac atgcagtagt 3181 tctactcatg cttatttcgc cattaagttg ggctggaacc atgactttcc agttccgtaa 3241 tccaaacttt ggtggtaacc caaataatgg cgctttttta ttaaatagcg ctcaggccca 3301 aaactcttat aaagatccga gctataacga tgactttggt attgaaacac cctcagcgtt 3361 agataacttt actcaggcca tccagtcaca aattttaggt gggctactgt cgaatattaa 3421 taccggtaaa ccgggccgca tggtgaccaa cgattatatt gtcgatattg ccaaccgcga 3481 tggtcaattg cagttgaacg tgacagatcg taaaaccgga caaacctcga ccatccaggt 3541 ttcgggttta caaaataact caaccgattt ttaagcccca gcttcataag gaaaataatc 3601 atgcagcgct tatttctttt ggttgccgtc atgttactga gcggatgctt aaccgccccg 3661 cctaaagaag ccgccagacc gacattaatg cctcgtgctc agagctacaa agatttgacc 3721 catctgccag cgccgacggg taaaatcttt gtttcggtat acaacattca ggacgaaacc 3781 gggcaattta aaccctaccc ggcaagtaac ttctccactg ctgttccgca aagcgccacg 3841 gcaatgctgg tcacggcact gaaagattct cgctggttta taccgctgga gcgccagggc 3901 ttacaaaacc tgcttaacga gcgcaagatt attcgtgcgg cacaagaaaa cggcacggtt 3961 gccattaata accgaatccc gctgcaatct ttaacggcgg caaatatcat ggttgaaggt 4021 tcgattatcg gttatgaaag caacgtcaaa tctggcgggg ttggggcaag atattttggc 4081 atcggtgccg acacgcaata ccagctcgat cagattgccg tgaacctgcg cgtcgtcaat 4141 gtgagtaccg gcgagatcct ttcttcggtg aacaccagta agacgatact ttcctatgaa 4201 gttcaggccg gggttttccg ctttattgac taccagcgct tgcttgaagg ggaagtgggt 4261 tacacctcga acgaacctgt tatgctgtgc ctgatgtcgg ctatcgaaac aggggtcatt 4321 ttcctgatta atgatggtat cgaccgtggt ctgtgggatt tgcaaaataa agcagaacgg 4381 cagaatgaca ttctggtgaa ataccgccat atgtcggttc caccggaatc ctgacac //