##gff-version 3
##sequence-region BBa_K1404001 1 4437
BBa_K1404001	.	engineered_region	1	1306	.	+	0	ID=BBa_K1404002;Name=BBa_K1404002
BBa_K1404001	.	CDS	96	1212	.	+	0	ID=annotation2385406;Name=NiCoT;Parent=BBa_K1404002
BBa_K1404001	.	promoter	1	76	.	+	0	ID=annotation2385405;Name=Ptac;Parent=BBa_K1404002
BBa_K1404001	.	sequence_feature	1212	1306	.	+	0	ID=annotation2385408;Name=B0014 terminator;Parent=BBa_K1404002
BBa_K1404001	.	ribosome_entry_site	83	95	.	+	0	ID=annotation2385407;Name=RBS Paris 2009;Parent=BBa_K1404002
BBa_K1404001	.	engineered_region	1315	4437	.	+	0	ID=BBa_K540000;Name=BBa_K540000
BBa_K1404001	.	CDS	3602	4435	.	+	0	ID=annotation2129034;Name=csgG;Parent=BBa_K540000
BBa_K1404001	.	sequence_alteration	2636	2641	.	+	0	ID=annotation2129029;Name=Mut2;Parent=BBa_K540000
BBa_K1404001	.	CDS	2266	2721	.	+	0	ID=annotation2129028;Name=csgA;Parent=BBa_K540000
BBa_K1404001	.	ribosome_entry_site	1756	1768	.	+	0	ID=annotation2129025;Name=perfect RBS1;Parent=BBa_K540000
BBa_K1404001	.	CDS	1771	2225	.	+	0	ID=annotation2129027;Name=csgB;Parent=BBa_K540000
BBa_K1404001	.	ribosome_entry_site	2729	2741	.	+	0	ID=annotation2129030;Name=perfect RBS2;Parent=BBa_K540000
BBa_K1404001	.	CDS	1715	1749	.	+	0	ID=annotation2129024;Name=rcnA;Parent=BBa_K540000
BBa_K1404001	.	sequence_alteration	3126	3131	.	+	0	ID=annotation2129032;Name=Mut3;Parent=BBa_K540000
BBa_K1404001	.	promoter	1736	1741	.	+	0	ID=annotation2129023;Name=-35rcnR;Parent=BBa_K540000
BBa_K1404001	.	CDS	1322	1594	.	+	0	ID=annotation2129021;Name=rcnR;Parent=BBa_K540000
BBa_K1404001	.	CDS	3159	3575	.	+	0	ID=annotation2129033;Name=csgF;Parent=BBa_K540000
BBa_K1404001	.	promoter	1709	1714	.	+	0	ID=annotation2129022;Name=-10rcnR;Parent=BBa_K540000
BBa_K1404001	.	sequence_alteration	1483	1489	.	+	0	ID=annotation2129026;Name=Mut1;Parent=BBa_K540000
BBa_K1404001	.	CDS	2745	3134	.	+	0	ID=annotation2129031;Name=csgE;Parent=BBa_K540000
>BBa_K1404001
aactgttaattagtaggccgagcatattacacaccttaacactcgcctattgttaaagtgtgtcctttgtctggtacta
gagaaggaggtatataatgggtggtgcggcggcgcgcctgcgccgccatccgagctttgtttatagcagcattgcggaa
ccgattatgcatctgaccaaaagcctgccgagcctgagcctgcgccgccgcattggtgcgctgtttgcgggtctgattg
cggcgaacattggtgtttgggtttgggcgtttagcctgtttcatgcgcagccgctgatgctgggtaccgcggttctggc
gtggggtctgggtctgcgccatgcggttgatgcggatcatattgcggcgattgataacgttacccgcaaactgatgcag
gatggtcagcgcccggttagcgttggtttttggtttgcgattggtcatagcggtattattgcgattgcgagcattatta
ttgcggttaccgcgagcgcgctgagccagtttggtgcgtttaaagaaattggtggtgttattgcgaccgttattagcgc
gctgtttctgtttaccattgcgggtatgaacctggttattctgcgcagcgtttggcgcacctttgcgcatgttcgcgcg
ggtggtagctatgcggaagatgatctggatctgctgctgggtggtcgcggtctgctgagccgcctgtttcgcccgatgt
ttcgcctggttaacaaaagctggcatatggcgccgctgggttttctgtttggtctgggttttgataccgcgaccgaagt
tgcgattctgggtatgagcgcgagccaggcggcggatggtctgagcattggtaccattctggttctgccggcgctgttt
gcggttggtatggcgctgattgataccgcggatggtgttgttatgctgggtgcgtatgaatgggcgtttgttaaaccga
ttcgcaaactgtattataacattaccattaccctgattagcgcgattgttgcgattgttattggtggtattgaaaccct
ggcgctgattggtgataaactggcgctgaccggtgcgccgtggcgcattgcgattgaactgggtgaaaactttaacggt
ctgggttttgcgattattggtctgtttgttttttgctggctggcgagctatgcgatttatcgctggaaacgctttgatg
aaattgaagttagcgttggtgcgtgatcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagag
aatataaaaagccagattattaatccggcttttttattattttactagagttattattatttgatatatgaatccagca
ccttcagaacgacatccagatcttcttcacgttttagctcatccccctggtgaacgatgtgttccgtcagatgaccttt
aatcacttcccgcatcagaccgtttaccgcgccacggatagcagcaatctgttgtaaaactgctgcgcattcgtgcggc
tcgtcgagcattttcttgagcgccacgacctggccctgaatcttactggcacgcgctttcagtttctgtttatcacgga
ttgtatgagacatggcaacacctggttaacaagaatatgaaaaatcatagcactattaatctactggggggtagtatca
ggtactgggggggagtagaatcagattgccgaattaatactaagaattattatcatgaccgaatttacaactcttcttc
agcaaggaaatactagaaggaggtatataatgtaaaaacaaattgttatttatgatgttaacaatactgggtgcgcctg
ggattgcagccgcagcaggttatgatttagctaattcagaatataacttcgcggtaaatgaattgagtaagtcttcatt
taatcaggcagccataattggtcaagctgggactaataatagtgctcagttacggcagggaggctcaaaacttttggcg
gttgttgcgcaagaaggtagtagcaaccgggcaaagattgaccagacaggagattataaccttgcatatattgatcagg
cgggcagtgccaacgatgccagtatttcgcaaggtgcttatggtaatactgcgatgattatccagaaaggttctggtaa
taaagcaaatattacacagtatggtactcaaaaaacggcaattgtagtgcagagacagtcgcaaatggctattcgcgtg
acacaacgttaatttccattcgacttttaaatcaatccgatgggggttttacatgaaacttttaaaagtagcagcaatt
gcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcg
gtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactga
tgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagc
tcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacgg
ttaaacagttcggtggtggcaacggtgcagcagttgaccagactgcatctaactcctccgtcaacgtgactcaggttgg
ctttggtaacaacgcgaccgctcatcagtactaactactagaaggaggtatataatgatgaaacgttatttacgctgga
ttgtggcggcagaatttctgttcgccgcagggaatcttcacgccgttgaggtagaagtcccgggattgctaactgacca
tactgtttcatctattggccatgatttttaccgagcctttagtgataaatgggaaagtgactatacgggtaacttaacg
attaatgaaaggcccagtgcacgatggggaagctggatcactataacggtcaatcaggacgttattttccagacttttt
tatttccgttgaaaagagacttcgagaaaactgtcgtctttgcactgattcaaactgaagaagcactaaatcgtcgcca
gataaatcaggcgttattaagtacgggcgatttggcgcatgatgaattttaaataaaaaattgttcggaggctgcaatg
cgtgtcaaacatgcagtagttctactcatgcttatttcgccattaagttgggctggaaccatgactttccagttccgta
atccaaactttggtggtaacccaaataatggcgcttttttattaaatagcgctcaggcccaaaactcttataaagatcc
gagctataacgatgactttggtattgaaacaccctcagcgttagataactttactcaggccatccagtcacaaatttta
ggtgggctactgtcgaatattaataccggtaaaccgggccgcatggtgaccaacgattatattgtcgatattgccaacc
gcgatggtcaattgcagttgaacgtgacagatcgtaaaaccggacaaacctcgaccatccaggtttcgggtttacaaaa
taactcaaccgatttttaagccccagcttcataaggaaaataatcatgcagcgcttatttcttttggttgccgtcatgt
tactgagcggatgcttaaccgccccgcctaaagaagccgccagaccgacattaatgcctcgtgctcagagctacaaaga
tttgacccatctgccagcgccgacgggtaaaatctttgtttcggtatacaacattcaggacgaaaccgggcaatttaaa
ccctacccggcaagtaacttctccactgctgttccgcaaagcgccacggcaatgctggtcacggcactgaaagattctc
gctggtttataccgctggagcgccagggcttacaaaacctgcttaacgagcgcaagattattcgtgcggcacaagaaaa
cggcacggttgccattaataaccgaatcccgctgcaatctttaacggcggcaaatatcatggttgaaggttcgattatc
ggttatgaaagcaacgtcaaatctggcggggttggggcaagatattttggcatcggtgccgacacgcaataccagctcg
atcagattgccgtgaacctgcgcgtcgtcaatgtgagtaccggcgagatcctttcttcggtgaacaccagtaagacgat
actttcctatgaagttcaggccggggttttccgctttattgactaccagcgcttgcttgaaggggaagtgggttacacc
tcgaacgaacctgttatgctgtgcctgatgtcggctatcgaaacaggggtcattttcctgattaatgatggtatcgacc
gtggtctgtgggatttgcaaaataaagcagaacggcagaatgacattctggtgaaataccgccatatgtcggttccacc
ggaatcctgacac