BBa_K540000 1 BBa_K540000 <i>rcn-csgBAEFG</i>, over-induces adherence in response to cobalt 2011-09-14T11:00:00Z 2015-05-08T01:12:38Z Direct synthesis from Genecust, using the sequence of the genes as found in E.Coli MC4100 genome. This parts associates the rcn promoter, whose expression increases with cobalt or nickel in the medium, with csgBAEFG, an operon that enables the production and secretion of curlis, which are adherent proteins. With this part, strains can become adherent in response to cobalt or nickel in the medium. false false _706_ 0 8419 9 It's complicated true Several internal iGEM restriction sites were initially present in the genes : EcoR1 in csgEFG, Pst1 in csgBA and rcn. They were eliminated in such a way that it does not affect the expression of the genes. false Melanie Geffroy, Philippe Thomas annotation2129034 1 csgG range2129034 1 2288 3121 annotation2129025 1 perfect RBS1 range2129025 1 442 454 annotation2129031 1 csgE range2129031 1 1431 1820 annotation2129024 1 rcnA range2129024 1 401 435 annotation2129033 1 csgF range2129033 1 1845 2261 annotation2129023 1 -35rcnR range2129023 1 422 427 annotation2129027 1 csgB range2129027 1 457 911 annotation2129022 1 -10rcnR range2129022 1 395 400 annotation2129029 1 Mut2 range2129029 1 1322 1327 annotation2129032 1 Mut3 range2129032 1 1812 1817 annotation2129021 1 rcnR range2129021 1 8 280 annotation2129026 1 Mut1 range2129026 1 169 175 annotation2129030 1 perfect RBS2 range2129030 1 1415 1427 annotation2129028 1 csgA range2129028 1 952 1407 BBa_K1404001 1 BBa_K1404001 Ptac-NiCoTB-rcn-csgBAEFG, turns E. coli into a functional Ni and Co biofilter 2014-09-22T11:00:00Z 2015-05-08T01:10:17Z [lien] and [lien] were cloned by 3A assembly. This part, when expressed in a rcnA E. coli strain, allows the formation of a functional Ni2+ and Co2+ biofilter capturing up to 3 mg of metal per g of bacterial dry weight when exposed to 20 ??M Ni2+ or Co2+, in only 10 mn. This is one of the best performances and the fastest reported until now. false false _1782_ 0 8419 9 In stock false Temp false Alexandre Duprey component2385428 1 BBa_K540000 component2385413 1 BBa_K1404002 annotation2385428 1 BBa_K540000 range2385428 1 1315 4437 annotation2385413 1 BBa_K1404002 range2385413 1 1 1306 BBa_K1404002 1 BBa_K1404002 Ptac-NiCoTB, improves nickel and cobalt internalization 2014-09-22T11:00:00Z 2015-07-23T03:01:16Z The promoter and the rest... This part comprises a strong E. coli promoter with... false false _1782_ 4206 8419 9 In stock false Temp false Alexandre Duprey annotation2385408 1 B0014 terminator range2385408 1 1212 1306 annotation2385405 1 Ptac range2385405 1 1 76 annotation2385406 1 NiCoT range2385406 1 96 1212 annotation2385407 1 RBS Paris 2009 range2385407 1 83 95 BBa_K540000_sequence 1 ttattattatttgatatatgaatccagcaccttcagaacgacatccagatcttcttcacgttttagctcatccccctggtgaacgatgtgttccgtcagatgacctttaatcacttcccgcatcagaccgtttaccgcgccacggatagcagcaatctgttgtaaaactgctgcgcattcgtgcggctcgtcgagcattttcttgagcgccacgacctggccctgaatcttactggcacgcgctttcagtttctgtttatcacggattgtatgagacatggcaacacctggttaacaagaatatgaaaaatcatagcactattaatctactggggggtagtatcaggtactgggggggagtagaatcagattgccgaattaatactaagaattattatcatgaccgaatttacaactcttcttcagcaaggaaatactagaaggaggtatataatgtaaaaacaaattgttatttatgatgttaacaatactgggtgcgcctgggattgcagccgcagcaggttatgatttagctaattcagaatataacttcgcggtaaatgaattgagtaagtcttcatttaatcaggcagccataattggtcaagctgggactaataatagtgctcagttacggcagggaggctcaaaacttttggcggttgttgcgcaagaaggtagtagcaaccgggcaaagattgaccagacaggagattataaccttgcatatattgatcaggcgggcagtgccaacgatgccagtatttcgcaaggtgcttatggtaatactgcgatgattatccagaaaggttctggtaataaagcaaatattacacagtatggtactcaaaaaacggcaattgtagtgcagagacagtcgcaaatggctattcgcgtgacacaacgttaatttccattcgacttttaaatcaatccgatgggggttttacatgaaacttttaaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgcagcagttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtactaactactagaaggaggtatataatgatgaaacgttatttacgctggattgtggcggcagaatttctgttcgccgcagggaatcttcacgccgttgaggtagaagtcccgggattgctaactgaccatactgtttcatctattggccatgatttttaccgagcctttagtgataaatgggaaagtgactatacgggtaacttaacgattaatgaaaggcccagtgcacgatggggaagctggatcactataacggtcaatcaggacgttattttccagacttttttatttccgttgaaaagagacttcgagaaaactgtcgtctttgcactgattcaaactgaagaagcactaaatcgtcgccagataaatcaggcgttattaagtacgggcgatttggcgcatgatgaattttaaataaaaaattgttcggaggctgcaatgcgtgtcaaacatgcagtagttctactcatgcttatttcgccattaagttgggctggaaccatgactttccagttccgtaatccaaactttggtggtaacccaaataatggcgcttttttattaaatagcgctcaggcccaaaactcttataaagatccgagctataacgatgactttggtattgaaacaccctcagcgttagataactttactcaggccatccagtcacaaattttaggtgggctactgtcgaatattaataccggtaaaccgggccgcatggtgaccaacgattatattgtcgatattgccaaccgcgatggtcaattgcagttgaacgtgacagatcgtaaaaccggacaaacctcgaccatccaggtttcgggtttacaaaataactcaaccgatttttaagccccagcttcataaggaaaataatcatgcagcgcttatttcttttggttgccgtcatgttactgagcggatgcttaaccgccccgcctaaagaagccgccagaccgacattaatgcctcgtgctcagagctacaaagatttgacccatctgccagcgccgacgggtaaaatctttgtttcggtatacaacattcaggacgaaaccgggcaatttaaaccctacccggcaagtaacttctccactgctgttccgcaaagcgccacggcaatgctggtcacggcactgaaagattctcgctggtttataccgctggagcgccagggcttacaaaacctgcttaacgagcgcaagattattcgtgcggcacaagaaaacggcacggttgccattaataaccgaatcccgctgcaatctttaacggcggcaaatatcatggttgaaggttcgattatcggttatgaaagcaacgtcaaatctggcggggttggggcaagatattttggcatcggtgccgacacgcaataccagctcgatcagattgccgtgaacctgcgcgtcgtcaatgtgagtaccggcgagatcctttcttcggtgaacaccagtaagacgatactttcctatgaagttcaggccggggttttccgctttattgactaccagcgcttgcttgaaggggaagtgggttacacctcgaacgaacctgttatgctgtgcctgatgtcggctatcgaaacaggggtcattttcctgattaatgatggtatcgaccgtggtctgtgggatttgcaaaataaagcagaacggcagaatgacattctggtgaaataccgccatatgtcggttccaccggaatcctgacac BBa_K1404002_sequence 1 aactgttaattagtaggccgagcatattacacaccttaacactcgcctattgttaaagtgtgtcctttgtctggtactagagaaggaggtatataatgggtggtgcggcggcgcgcctgcgccgccatccgagctttgtttatagcagcattgcggaaccgattatgcatctgaccaaaagcctgccgagcctgagcctgcgccgccgcattggtgcgctgtttgcgggtctgattgcggcgaacattggtgtttgggtttgggcgtttagcctgtttcatgcgcagccgctgatgctgggtaccgcggttctggcgtggggtctgggtctgcgccatgcggttgatgcggatcatattgcggcgattgataacgttacccgcaaactgatgcaggatggtcagcgcccggttagcgttggtttttggtttgcgattggtcatagcggtattattgcgattgcgagcattattattgcggttaccgcgagcgcgctgagccagtttggtgcgtttaaagaaattggtggtgttattgcgaccgttattagcgcgctgtttctgtttaccattgcgggtatgaacctggttattctgcgcagcgtttggcgcacctttgcgcatgttcgcgcgggtggtagctatgcggaagatgatctggatctgctgctgggtggtcgcggtctgctgagccgcctgtttcgcccgatgtttcgcctggttaacaaaagctggcatatggcgccgctgggttttctgtttggtctgggttttgataccgcgaccgaagttgcgattctgggtatgagcgcgagccaggcggcggatggtctgagcattggtaccattctggttctgccggcgctgtttgcggttggtatggcgctgattgataccgcggatggtgttgttatgctgggtgcgtatgaatgggcgtttgttaaaccgattcgcaaactgtattataacattaccattaccctgattagcgcgattgttgcgattgttattggtggtattgaaaccctggcgctgattggtgataaactggcgctgaccggtgcgccgtggcgcattgcgattgaactgggtgaaaactttaacggtctgggttttgcgattattggtctgtttgttttttgctggctggcgagctatgcgatttatcgctggaaacgctttgatgaaattgaagttagcgttggtgcgtgatcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_K1404001_sequence 1 aactgttaattagtaggccgagcatattacacaccttaacactcgcctattgttaaagtgtgtcctttgtctggtactagagaaggaggtatataatgggtggtgcggcggcgcgcctgcgccgccatccgagctttgtttatagcagcattgcggaaccgattatgcatctgaccaaaagcctgccgagcctgagcctgcgccgccgcattggtgcgctgtttgcgggtctgattgcggcgaacattggtgtttgggtttgggcgtttagcctgtttcatgcgcagccgctgatgctgggtaccgcggttctggcgtggggtctgggtctgcgccatgcggttgatgcggatcatattgcggcgattgataacgttacccgcaaactgatgcaggatggtcagcgcccggttagcgttggtttttggtttgcgattggtcatagcggtattattgcgattgcgagcattattattgcggttaccgcgagcgcgctgagccagtttggtgcgtttaaagaaattggtggtgttattgcgaccgttattagcgcgctgtttctgtttaccattgcgggtatgaacctggttattctgcgcagcgtttggcgcacctttgcgcatgttcgcgcgggtggtagctatgcggaagatgatctggatctgctgctgggtggtcgcggtctgctgagccgcctgtttcgcccgatgtttcgcctggttaacaaaagctggcatatggcgccgctgggttttctgtttggtctgggttttgataccgcgaccgaagttgcgattctgggtatgagcgcgagccaggcggcggatggtctgagcattggtaccattctggttctgccggcgctgtttgcggttggtatggcgctgattgataccgcggatggtgttgttatgctgggtgcgtatgaatgggcgtttgttaaaccgattcgcaaactgtattataacattaccattaccctgattagcgcgattgttgcgattgttattggtggtattgaaaccctggcgctgattggtgataaactggcgctgaccggtgcgccgtggcgcattgcgattgaactgggtgaaaactttaacggtctgggttttgcgattattggtctgtttgttttttgctggctggcgagctatgcgatttatcgctggaaacgctttgatgaaattgaagttagcgttggtgcgtgatcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagttattattatttgatatatgaatccagcaccttcagaacgacatccagatcttcttcacgttttagctcatccccctggtgaacgatgtgttccgtcagatgacctttaatcacttcccgcatcagaccgtttaccgcgccacggatagcagcaatctgttgtaaaactgctgcgcattcgtgcggctcgtcgagcattttcttgagcgccacgacctggccctgaatcttactggcacgcgctttcagtttctgtttatcacggattgtatgagacatggcaacacctggttaacaagaatatgaaaaatcatagcactattaatctactggggggtagtatcaggtactgggggggagtagaatcagattgccgaattaatactaagaattattatcatgaccgaatttacaactcttcttcagcaaggaaatactagaaggaggtatataatgtaaaaacaaattgttatttatgatgttaacaatactgggtgcgcctgggattgcagccgcagcaggttatgatttagctaattcagaatataacttcgcggtaaatgaattgagtaagtcttcatttaatcaggcagccataattggtcaagctgggactaataatagtgctcagttacggcagggaggctcaaaacttttggcggttgttgcgcaagaaggtagtagcaaccgggcaaagattgaccagacaggagattataaccttgcatatattgatcaggcgggcagtgccaacgatgccagtatttcgcaaggtgcttatggtaatactgcgatgattatccagaaaggttctggtaataaagcaaatattacacagtatggtactcaaaaaacggcaattgtagtgcagagacagtcgcaaatggctattcgcgtgacacaacgttaatttccattcgacttttaaatcaatccgatgggggttttacatgaaacttttaaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgcagcagttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtactaactactagaaggaggtatataatgatgaaacgttatttacgctggattgtggcggcagaatttctgttcgccgcagggaatcttcacgccgttgaggtagaagtcccgggattgctaactgaccatactgtttcatctattggccatgatttttaccgagcctttagtgataaatgggaaagtgactatacgggtaacttaacgattaatgaaaggcccagtgcacgatggggaagctggatcactataacggtcaatcaggacgttattttccagacttttttatttccgttgaaaagagacttcgagaaaactgtcgtctttgcactgattcaaactgaagaagcactaaatcgtcgccagataaatcaggcgttattaagtacgggcgatttggcgcatgatgaattttaaataaaaaattgttcggaggctgcaatgcgtgtcaaacatgcagtagttctactcatgcttatttcgccattaagttgggctggaaccatgactttccagttccgtaatccaaactttggtggtaacccaaataatggcgcttttttattaaatagcgctcaggcccaaaactcttataaagatccgagctataacgatgactttggtattgaaacaccctcagcgttagataactttactcaggccatccagtcacaaattttaggtgggctactgtcgaatattaataccggtaaaccgggccgcatggtgaccaacgattatattgtcgatattgccaaccgcgatggtcaattgcagttgaacgtgacagatcgtaaaaccggacaaacctcgaccatccaggtttcgggtttacaaaataactcaaccgatttttaagccccagcttcataaggaaaataatcatgcagcgcttatttcttttggttgccgtcatgttactgagcggatgcttaaccgccccgcctaaagaagccgccagaccgacattaatgcctcgtgctcagagctacaaagatttgacccatctgccagcgccgacgggtaaaatctttgtttcggtatacaacattcaggacgaaaccgggcaatttaaaccctacccggcaagtaacttctccactgctgttccgcaaagcgccacggcaatgctggtcacggcactgaaagattctcgctggtttataccgctggagcgccagggcttacaaaacctgcttaacgagcgcaagattattcgtgcggcacaagaaaacggcacggttgccattaataaccgaatcccgctgcaatctttaacggcggcaaatatcatggttgaaggttcgattatcggttatgaaagcaacgtcaaatctggcggggttggggcaagatattttggcatcggtgccgacacgcaataccagctcgatcagattgccgtgaacctgcgcgtcgtcaatgtgagtaccggcgagatcctttcttcggtgaacaccagtaagacgatactttcctatgaagttcaggccggggttttccgctttattgactaccagcgcttgcttgaaggggaagtgggttacacctcgaacgaacctgttatgctgtgcctgatgtcggctatcgaaacaggggtcattttcctgattaatgatggtatcgaccgtggtctgtgggatttgcaaaataaagcagaacggcagaatgacattctggtgaaataccgccatatgtcggttccaccggaatcctgacac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z