LOCUS       BBa_K1404008             607 bp    DNA     linear       25-Nov-2024
DEFINITION  p70-CsgA-His*2, double His-tagged curli generator
ACCESSION   BBa_K1404008
VERSION     BBa_K1404008.1  
FEATURES             Location/Qualifiers
     promoter        1..70
                     /label=BBa_K342000
     RBS             79..91
                     /label=RBS Paris 2009
     misc_feature    92..151
                     /label=SEC signal
     CDS             92..607
                     /label=CsgA-His2
     misc_feature    152..217
                     /label=CsgD signal
     misc_feature    218..286
                     /label=Beta sheet
     misc_feature    287..352
                     /label=Beta sheet2
     misc_feature    353..421
                     /label=Beta sheet
     misc_feature    422..487
                     /label=Beta sheet4
     misc_feature    461..463
                     /label=GCT into GCC
     misc_feature    488..544
                     /label=Beta sheet5
     misc_feature    545..556
                     /label=Linker
     misc_feature    557..574
                     /label=His tag
     misc_feature    575..586
                     /label=linker
     misc_feature    587..604
                     /label=His tag
ORIGIN
        1 gaactaaaaa agaaaaatac aacgcgcggg tgagttatta aaaatatttc cgcagacata
       61 ctttccatcg tactagagaa ggaggtatat aatgaaactt ttaaaagtag cagcaattgc
      121 agcaatcgta ttctccggta gcgctctggc aggtgttgtt cctcagtacg gcggcggcgg
      181 taaccacggt ggtggcggta ataatagcgg cccaaattct gagctgaaca tttaccagta
      241 cggtggcggt aactctgcac ttgctctgca aactgatgcc cgtaactctg acttgactat
      301 tacccagcat ggcggcggta atggtgcaga tgttggtcag ggctcagatg acagctcaat
      361 cgatctgacc caacgtggct tcggtaacag cgctactctt gatcagtgga acggcaaaaa
      421 ttctgaaatg acggttaaac agttcggtgg tggcaacggt gccgcagttg accagactgc
      481 atctaactcc tccgtcaacg tgactcaggt tggctttggt aacaacgcga ccgctcatca
      541 gtacggcggt gggggtcatc atcaccatca ccacggtgga gggggtcacc atcatcatca
      601 ccattaa
//