BBa_K1406001 1 BBa_K1406001 pLAC promoter + strong RBS 2014-10-08T11:00:00Z 2015-05-08T01:10:17Z phage lamda A combinatorial part of R0011, B0034, and a NheI cutsite. This allows a in-frame, orientated insertion of a biobrick part behind the pLac promoter and the strong RBS using the restriction enzymes NheI and PstI. false false _1784_ 0 17066 9 It's complicated false We were hoping to make a high-expressing, directional insertion system in which we used enzymes which were compatible with the BioBrick standard and as an end product form a BioBrick. false Sierra Tackett annotation2408553 1 conserved range2408553 1 68 71 annotation2408549 1 lac O1 range2408549 1 3 19 annotation2408550 1 -35 range2408550 1 20 25 annotation2408554 1 BBa_B0034 range2408554 1 64 75 annotation2408548 1 BBa_R0011 range2408548 1 1 54 annotation2408551 1 lac O1 range2408551 1 26 42 annotation2408552 1 -10 range2408552 1 43 48 BBa_K1406001_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacaaaagaggaattgacattgtgagcggataacaagatactgagcacaaaagaggagaaaaatgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z