LOCUS       BBa_K1407008            1969 bp    DNA     linear       03-May-2024
DEFINITION  constitutive production of LasR+LasR/PAI1 Inducible promoter 
            +RBS(B0034)+GFP+Terminator
ACCESSION   BBa_K1407008
VERSION     BBa_K1407008.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..784
                     /label=BBa_C0179
     stem_loop       804..847
                     /label=stem_loop
     misc_feature    793..872
                     /label=BBa_B0010
     terminator      793..872
                     /label=BBa_B0010
     stem_loop       888..907
                     /label=T7 TE
     misc_feature    914..914
                     /label=stop
     polyA_site      908..921
                     /label=polya
     misc_feature    881..921
                     /label=BBa_B0012
     terminator      881..921
                     /label=BBa_B0012
     terminator      793..921
                     /label=BBa_B0015
     misc_feature    1..921
                     /label=BBa_K553003
     misc_feature    975..992
                     /label=OP2
     misc_feature    1035..1054
                     /label=OP1
     promoter        1046..1051
                     /label=-35
     promoter        1069..1074
                     /label=-10
     promoter        930..1086
                     /label=BBa_R0079
     misc_feature    1099..1102
                     /label=conserved
     RBS             1095..1106
                     /label=BBa_B0034
     CDS             1113..1832
                     /label=GFP protein
     CDS             1113..1832
                     /label=BBa_E0040
     misc_feature    930..1832
                     /label=BBa_K575009
     stem_loop       1852..1895
                     /label=stem_loop
     misc_feature    1841..1920
                     /label=BBa_B0010
     terminator      1841..1920
                     /label=BBa_B0010
     stem_loop       1936..1955
                     /label=T7 TE
     misc_feature    1962..1962
                     /label=stop
     polyA_site      1956..1969
                     /label=polya
     misc_feature    1929..1969
                     /label=BBa_B0012
     terminator      1929..1969
                     /label=BBa_B0012
     terminator      1841..1969
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatggccttg gttgacggtt ttcttgagct ggaacgctca agtggaaaat tggagtggag
      121 cgccatcctc cagaagatgg cgagcgacct tggattctcg aagatcctgt tcggcctgtt
      181 gcctaaggac agccaggact acgagaacgc cttcatcgtc ggcaactacc cggccgcctg
      241 gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac ccgacggtca gtcactgtac
      301 ccagagcgta ctgccgattt tctgggaacc gtccatctac cagacgcgaa agcagcacga
      361 gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg ctgaccatgc cgctgcatgg
      421 tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa gcggaaaacc gggccgaggc
      481 caaccgtttc atagagtcgg tcctgccgac cctgtggatg ctcaaggact acgcactgca
      541 aagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa ccggtggttc tgaccagccg
      601 ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc agttgggaga tatcggttat
      661 ctgcaactgc tcggaagcca atgtgaactt ccatatggga aatattcggc ggaagttcgg
      721 tgtgacctcc cgccgcgtag cggccattat ggccgttaat ttgggtctta ttactctcta
      781 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      841 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      901 gtgggccttt ctgcgtttat atactagagg cccctcgctg agcgcgtccc ggagctgggg
      961 gcaacctagc tgccacctgc ttttctgcta gctattccag cgaaaacata cagatttccg
     1021 gcgaaatcaa ggctacctgc cagttctggc aggtttggcc gcgggttctt tttggtacac
     1081 gaaagctact agagaaagag gagaaatact agatgcgtaa aggagaagaa cttttcactg
     1141 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca
     1201 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta
     1261 ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat
     1321 gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg
     1381 aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg
     1441 ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt
     1501 ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac tcacacaatg
     1561 tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca
     1621 acattgaaga tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg
     1681 atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag
     1741 atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta
     1801 cacatggcat ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga
     1861 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1921 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//