constitutive production of LasR+LasR/PAI1 Inducible promoter +RBS(B0034)+GFP+Terminator
It's composed of three sections:constitutive production of LasR,LasR/PAI1 inducible promoter with RBS (B0034) and GFP,terminator.By adding PAI1,the part could be inducibled and expresses GFP protein.
Double terminator consisting of BBa_B0010 and BBa_B0012
LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP
The inducable Promoter for the LasR/PAI1 complex attached to RBS (B0034) and GFP.
Need to ensure no leaky transciption of GFP.
constitutive production of LasR trans-activator
Giulio Bernardinelli, Veronica Parisi, Francesca Cesaratto
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
GFP (mut3b) [note that this part does not have a barcode]
"Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Lynn Rust, Everett Pesci, and Barbara Iglewski
Binding region for LasR protein (positive regulation)
lasR activator from P. aeruginosa PAO1(no LVA)
Conversion of the iGEM parts registry to SBOL2.1