LOCUS       BBa_K1407009            1875 bp    DNA     linear       26-Nov-2024
DEFINITION  RhlR protein Generator +RhlR/PAI2 Inducible promoter+RBS(B0030)+RFP
            +Terminator
ACCESSION   BBa_K1407009
VERSION     BBa_K1407009.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..741
                     /label=rhlR
     misc_feature    742..774
                     /label=LVA
     misc_feature    781..805
                     /label=Help:Barcodes
     CDS             19..780
                     /label=BBa_C0071
     stem_loop       825..868
                     /label=stem_loop
     misc_feature    814..893
                     /label=BBa_B0010
     terminator      814..893
                     /label=BBa_B0010
     stem_loop       909..928
                     /label=T7 TE
     misc_feature    935..935
                     /label=stop
     polyA_site      929..942
                     /label=polya
     misc_feature    902..942
                     /label=BBa_B0012
     terminator      902..942
                     /label=BBa_B0012
     misc_feature    1..942
                     /label=BBa_I0466
     misc_feature    951..970
                     /label=RhlR
     promoter        969..974
                     /label=-35
     promoter        992..997
                     /label=-10
     misc_feature    1003..1003
                     /label=start
     promoter        951..1003
                     /label=BBa_R0071
     RBS             1012..1026
                     /label=RBS-1\Strong
     RBS             1019..1023
                     /label=RBS
     misc_feature    1012..1026
                     /label=BBa_B0030
     RBS             1012..1026
                     /label=BBa_B0030
     CDS             1033..1707
                     /label=mrfp1
     misc_feature    1714..1738
                     /label=Help:Barcodes
     CDS             1033..1738
                     /label=BBa_E1010
     misc_feature    951..1738
                     /label=BBa_K575014
     stem_loop       1758..1801
                     /label=stem_loop
     misc_feature    1747..1826
                     /label=BBa_B0010
     terminator      1747..1826
                     /label=BBa_B0010
     stem_loop       1842..1861
                     /label=T7 TE
     misc_feature    1868..1868
                     /label=stop
     polyA_site      1862..1875
                     /label=polya
     misc_feature    1835..1875
                     /label=BBa_B0012
     terminator      1835..1875
                     /label=BBa_B0012
     terminator      1747..1875
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagat gaggaatgac ggaggctttt tgctgtggtg ggacggtttg
       61 cgtagcgaga tgcagccgat ccacgacagc cagggcgtgt tcgccgtcct ggaaaaggaa
      121 gtgcggcgcc tgggcttcga ttactacgcc tatggcgtgc gccacacgat tcccttcacc
      181 cggccgaaga ccgaggtcca tggcacctat cccaaggcct ggctggagcg ataccagatg
      241 cagaactacg gggccgtgga tccggcgatc ctcaacggcc tgcgctcctc ggaaatggtg
      301 gtctggagcg acagcctgtt cgaccagagc cggatgctct ggaacgaggc tcgcgattgg
      361 ggcctctgtg tcggcgcgac cttgccgatc cgcgcgccga acaatttgct cagcgtgctt
      421 tccgtggcgc gcgaccagca gaacatctcc agcttcgagc gcgaggaaat ccgcctgcgg
      481 ctgcgttgca tgatcgagtt gctgacccag aagctgaccg acctggagca tccgatgctg
      541 atgtccaacc cggtctgcct gagccatcgc gaacgcgaga tcctgcaatg gaccgccgac
      601 ggcaagagtt ccggggaaat cgccatcatc ctgagcatct ccgagagcac ggtgaacttc
      661 caccacaaga acatccagaa gaagttcgac gcgccgaaca agacgctggc tgccgcctac
      721 gccgcggcgc tgggtctcat cgctgcaaac gacgaaaact acgctttagt agcttaataa
      781 cactgatagt gctagtgtag atcactacta gagccaggca tcaaataaaa cgaaaggctc
      841 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
      901 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag tcctgtgaaa
      961 tctggcagtt accgttagct ttcgaattgg ctaaaaagtg ttctactaga gattaaagag
     1021 gagaaatact agatggcttc ctccgaagac gttatcaaag agttcatgcg tttcaaagtt
     1081 cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt
     1141 ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc gctgccgttc
     1201 gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt taaacacccg
     1261 gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt
     1321 atgaacttcg aagacggtgg tgttgttacc gttacccagg actcctccct gcaagacggt
     1381 gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgacgg tccggttatg
     1441 cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga agacggtgct
     1501 ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa
     1561 gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta caaaaccgac
     1621 atcaaactgg acatcacctc ccacaacgaa gactacacca tcgttgaaca gtacgaacgt
     1681 gctgaaggtc gtcactccac cggtgcttaa taacgctgat agtgctagtg tagatcgcta
     1741 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1801 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1861 ctttctgcgt ttata
//