##gff-version 3
##sequence-region BBa_K1407010 1 1958
BBa_K1407010	.	terminator	1830	1958	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1407010	.	terminator	1830	1909	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1407010	.	stem_loop	1841	1884	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1407010	.	engineered_region	1830	1909	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1407010	.	terminator	1918	1958	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1407010	.	polyA_site	1945	1958	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1407010	.	stem_loop	1925	1944	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1407010	.	engineered_region	1918	1958	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1407010	.	stop_codon	1951	1951	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1407010	.	engineered_region	1	921	.	+	0	ID=BBa_K553003;Name=BBa_K553003
BBa_K1407010	.	terminator	793	921	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K553003
BBa_K1407010	.	terminator	793	872	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1407010	.	stem_loop	804	847	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1407010	.	engineered_region	793	872	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1407010	.	terminator	881	921	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1407010	.	polyA_site	908	921	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1407010	.	stem_loop	888	907	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1407010	.	engineered_region	881	921	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1407010	.	stop_codon	914	914	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1407010	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K553003
BBa_K1407010	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1407010	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K553003
BBa_K1407010	.	CDS	62	784	.	+	0	ID=BBa_C0179;Name=BBa_C0179;Parent=BBa_K553003
BBa_K1407010	.	engineered_region	930	1821	.	+	0	ID=BBa_K575010;Name=BBa_K575010
BBa_K1407010	.	CDS	1116	1821	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K575010
BBa_K1407010	.	CDS	1116	1790	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1407010	.	engineered_tag	1797	1821	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1407010	.	ribosome_entry_site	1095	1109	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K575010
BBa_K1407010	.	ribosome_entry_site	1095	1109	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1407010	.	ribosome_entry_site	1102	1106	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1407010	.	engineered_region	1095	1109	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1407010	.	promoter	930	1086	.	+	0	ID=BBa_R0079;Name=BBa_R0079;Parent=BBa_K575010
BBa_K1407010	.	non_covalent_binding_site	975	992	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_K1407010	.	non_covalent_binding_site	1035	1054	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_K1407010	.	promoter	1069	1074	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_K1407010	.	promoter	1046	1051	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
>BBa_K1407010
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggccttggttgacggt
tttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattct
cgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgc
ctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccg
attttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgt
atgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggc
cgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccgga
ctggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcg
gcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcg
gaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataatactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcccctcgctgagcgcgtc
ccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaat
caaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctactagagattaaagaggag
aaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacg
gtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaa
aggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccg
gctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtg
gtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaactt
cccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggt
gctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctaca
tggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagacta
caccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtaga
tcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata