##gff-version 3
##sequence-region BBa_K1407011 1 1918
BBa_K1407011	.	engineered_region	994	1781	.	+	0	ID=BBa_K575014;Name=BBa_K575014
BBa_K1407011	.	ribosome_entry_site	1055	1069	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K575014
BBa_K1407011	.	ribosome_entry_site	1062	1066	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1407011	.	ribosome_entry_site	1055	1069	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1407011	.	engineered_region	1055	1069	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1407011	.	promoter	994	1046	.	+	0	ID=BBa_R0071;Name=BBa_R0071;Parent=BBa_K575014
BBa_K1407011	.	promoter	1035	1040	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_K1407011	.	start_codon	1046	1046	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_K1407011	.	non_covalent_binding_site	994	1013	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_K1407011	.	promoter	1012	1017	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_K1407011	.	CDS	1076	1781	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K575014
BBa_K1407011	.	engineered_tag	1757	1781	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1407011	.	CDS	1076	1750	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1407011	.	terminator	1790	1918	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1407011	.	terminator	1878	1918	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1407011	.	polyA_site	1905	1918	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1407011	.	engineered_region	1878	1918	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1407011	.	stem_loop	1885	1904	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1407011	.	stop_codon	1911	1911	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1407011	.	terminator	1790	1869	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1407011	.	engineered_region	1790	1869	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1407011	.	stem_loop	1801	1844	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1407011	.	promoter	1	35	.	+	0	ID=BBa_J23119;Name=BBa_J23119
BBa_K1407011	.	engineered_region	44	985	.	+	0	ID=BBa_I0466;Name=BBa_I0466
BBa_K1407011	.	terminator	857	936	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0466
BBa_K1407011	.	engineered_region	857	936	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1407011	.	stem_loop	868	911	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1407011	.	terminator	945	985	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0466
BBa_K1407011	.	polyA_site	972	985	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1407011	.	engineered_region	945	985	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1407011	.	stem_loop	952	971	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1407011	.	stop_codon	978	978	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1407011	.	CDS	62	823	.	+	0	ID=BBa_C0071;Name=BBa_C0071;Parent=BBa_I0466
BBa_K1407011	.	engineered_tag	824	848	.	+	0	ID=annotation2213995;Name=Help:Barcodes;Parent=BBa_C0071
BBa_K1407011	.	CDS	62	784	.	+	0	ID=annotation301192;Name=rhlR;Parent=BBa_C0071
BBa_K1407011	.	tag	785	817	.	+	0	ID=annotation306523;Name=LVA;Parent=BBa_C0071
BBa_K1407011	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0466
BBa_K1407011	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K1407011
ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgaggaatgacggaggc
tttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaa
aggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccga
ggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctc
aacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctc
gcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcg
cgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccag
aagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaat
ggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaa
gaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatcgctgca
aacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaat
aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttatatactagagtcctgtgaaatctggcagttaccgttagctttcg
aattggctaaaaagtgttctactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagt
tcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtcc
gtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccg
cagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaag
gtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacgg
tgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggt
tgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaag
acggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaac
cgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcac
tccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttata