LOCUS       BBa_K1407012            1860 bp    DNA     linear       03-May-2024
DEFINITION  constitutive promoter+RhlR protein generator(LVA-)+RhlR/PAI2 
            Inducible promoter+RBS(B0030)+RFP+Termi
ACCESSION   BBa_K1407012
VERSION     BBa_K1407012.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..790
                     /label=BBa_C0171
     stem_loop       810..853
                     /label=stem_loop
     misc_feature    799..878
                     /label=BBa_B0010
     terminator      799..878
                     /label=BBa_B0010
     stem_loop       894..913
                     /label=T7 TE
     misc_feature    920..920
                     /label=stop
     polyA_site      914..927
                     /label=polya
     misc_feature    887..927
                     /label=BBa_B0012
     terminator      887..927
                     /label=BBa_B0012
     misc_feature    44..927
                     /label=BBa_I1466
     misc_feature    936..955
                     /label=RhlR
     promoter        954..959
                     /label=-35
     promoter        977..982
                     /label=-10
     misc_feature    988..988
                     /label=start
     promoter        936..988
                     /label=BBa_R0071
     RBS             997..1011
                     /label=RBS-1\Strong
     RBS             1004..1008
                     /label=RBS
     misc_feature    997..1011
                     /label=BBa_B0030
     RBS             997..1011
                     /label=BBa_B0030
     CDS             1018..1692
                     /label=mrfp1
     misc_feature    1699..1723
                     /label=Help:Barcodes
     CDS             1018..1723
                     /label=BBa_E1010
     misc_feature    936..1723
                     /label=BBa_K575014
     stem_loop       1743..1786
                     /label=stem_loop
     misc_feature    1732..1811
                     /label=BBa_B0010
     terminator      1732..1811
                     /label=BBa_B0010
     stem_loop       1827..1846
                     /label=T7 TE
     misc_feature    1853..1853
                     /label=stop
     polyA_site      1847..1860
                     /label=polya
     misc_feature    1820..1860
                     /label=BBa_B0012
     terminator      1820..1860
                     /label=BBa_B0012
     terminator      1732..1860
                     /label=BBa_B0015
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta
       61 gatgaggaat gacggaggct ttttgctgtg gtgggacggt ttgcgtagcg agatgcagcc
      121 gatccacgac agccagggcg tgttcgccgt cctggaaaag gaagtgcggc gcctgggctt
      181 cgattactac gcctatggcg tgcgccacac gattcccttc acccggccga agaccgaggt
      241 ccatggcacc tatcccaagg cctggctgga gcgataccag atgcagaact acggggccgt
      301 ggatccggcg atcctcaacg gcctgcgctc ctcggaaatg gtggtctgga gcgacagcct
      361 gttcgaccag agccggatgc tctggaacga ggctcgcgat tggggcctct gtgtcggcgc
      421 gaccttgccg atccgcgcgc cgaacaattt gctcagcgtg ctttccgtgg cgcgcgacca
      481 gcagaacatc tccagcttcg agcgcgagga aatccgcctg cggctgcgtt gcatgatcga
      541 gttgctgacc cagaagctga ccgacctgga gcatccgatg ctgatgtcca acccggtctg
      601 cctgagccat cgcgaacgcg agatcctgca atggaccgcc gacggcaaga gttccgggga
      661 aatcgccatc atcctgagca tctccgagag cacggtgaac ttccaccaca agaacatcca
      721 gaagaagttc gacgcgccga acaagacgct ggctgccgcc tacgccgcgg cgctgggtct
      781 catctaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      841 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
      901 cttcgggtgg gcctttctgc gtttatatac tagagtcctg tgaaatctgg cagttaccgt
      961 tagctttcga attggctaaa aagtgttcta ctagagatta aagaggagaa atactagatg
     1021 gcttcctccg aagacgttat caaagagttc atgcgtttca aagttcgtat ggaaggttcc
     1081 gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc
     1141 cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg
     1201 tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga catcccggac
     1261 tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac
     1321 ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt catctacaaa
     1381 gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg
     1441 ggttgggaag cttccaccga acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc
     1501 aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa aaccacctac
     1561 atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa actggacatc
     1621 acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga aggtcgtcac
     1681 tccaccggtg cttaataacg ctgatagtgc tagtgtagat cgctactaga gccaggcatc
     1741 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1801 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//