LOCUS BBa_K1407013 3884 bp DNA linear 07-May-2024 DEFINITION LasR+LasR/PAI1 Inducible promoter+RFP+RhlR+RhlR/PAI2 Inducible promoter+RFP ACCESSION BBa_K1407013 VERSION BBa_K1407013.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..784 /label=BBa_C0179 stem_loop 804..847 /label=stem_loop misc_feature 793..872 /label=BBa_B0010 terminator 793..872 /label=BBa_B0010 stem_loop 888..907 /label=T7 TE misc_feature 914..914 /label=stop polyA_site 908..921 /label=polya misc_feature 881..921 /label=BBa_B0012 terminator 881..921 /label=BBa_B0012 terminator 793..921 /label=BBa_B0015 misc_feature 1..921 /label=BBa_K553003 misc_feature 975..992 /label=OP2 misc_feature 1035..1054 /label=OP1 promoter 1046..1051 /label=-35 promoter 1069..1074 /label=-10 promoter 930..1086 /label=BBa_R0079 RBS 1095..1109 /label=RBS-1\Strong RBS 1102..1106 /label=RBS misc_feature 1095..1109 /label=BBa_B0030 RBS 1095..1109 /label=BBa_B0030 CDS 1116..1790 /label=mrfp1 misc_feature 1797..1821 /label=Help:Barcodes CDS 1116..1821 /label=BBa_E1010 misc_feature 930..1821 /label=BBa_K575010 stem_loop 1841..1884 /label=stem_loop misc_feature 1830..1909 /label=BBa_B0010 terminator 1830..1909 /label=BBa_B0010 stem_loop 1925..1944 /label=T7 TE misc_feature 1951..1951 /label=stop polyA_site 1945..1958 /label=polya misc_feature 1918..1958 /label=BBa_B0012 terminator 1918..1958 /label=BBa_B0012 terminator 1830..1958 /label=BBa_B0015 misc_feature 1..1958 /label=BBa_K1407010 promoter 1967..2001 /label=BBa_J23119 misc_feature 2014..2017 /label=conserved RBS 2010..2021 /label=BBa_B0034 CDS 2028..2750 /label=rhlR misc_feature 2751..2783 /label=LVA misc_feature 2790..2814 /label=Help:Barcodes CDS 2028..2789 /label=BBa_C0071 stem_loop 2834..2877 /label=stem_loop misc_feature 2823..2902 /label=BBa_B0010 terminator 2823..2902 /label=BBa_B0010 stem_loop 2918..2937 /label=T7 TE misc_feature 2944..2944 /label=stop polyA_site 2938..2951 /label=polya misc_feature 2911..2951 /label=BBa_B0012 terminator 2911..2951 /label=BBa_B0012 misc_feature 2010..2951 /label=BBa_I0466 misc_feature 2960..2979 /label=RhlR promoter 2978..2983 /label=-35 promoter 3001..3006 /label=-10 misc_feature 3012..3012 /label=start promoter 2960..3012 /label=BBa_R0071 RBS 3021..3035 /label=RBS-1\Strong RBS 3028..3032 /label=RBS misc_feature 3021..3035 /label=BBa_B0030 RBS 3021..3035 /label=BBa_B0030 CDS 3042..3716 /label=mrfp1 misc_feature 3723..3747 /label=Help:Barcodes CDS 3042..3747 /label=BBa_E1010 misc_feature 2960..3747 /label=BBa_K575014 stem_loop 3767..3810 /label=stem_loop misc_feature 3756..3835 /label=BBa_B0010 terminator 3756..3835 /label=BBa_B0010 stem_loop 3851..3870 /label=T7 TE misc_feature 3877..3877 /label=stop polyA_site 3871..3884 /label=polya misc_feature 3844..3884 /label=BBa_B0012 terminator 3844..3884 /label=BBa_B0012 terminator 3756..3884 /label=BBa_B0015 misc_feature 1967..3884 /label=BBa_K1407011 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta 61 gatggccttg gttgacggtt ttcttgagct ggaacgctca agtggaaaat tggagtggag 121 cgccatcctc cagaagatgg cgagcgacct tggattctcg aagatcctgt tcggcctgtt 181 gcctaaggac agccaggact acgagaacgc cttcatcgtc ggcaactacc cggccgcctg 241 gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac ccgacggtca gtcactgtac 301 ccagagcgta ctgccgattt tctgggaacc gtccatctac cagacgcgaa agcagcacga 361 gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg ctgaccatgc cgctgcatgg 421 tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa gcggaaaacc gggccgaggc 481 caaccgtttc atagagtcgg tcctgccgac cctgtggatg ctcaaggact acgcactgca 541 aagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa ccggtggttc tgaccagccg 601 ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc agttgggaga tatcggttat 661 ctgcaactgc tcggaagcca atgtgaactt ccatatggga aatattcggc ggaagttcgg 721 tgtgacctcc cgccgcgtag cggccattat ggccgttaat ttgggtctta ttactctcta 781 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 841 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 901 gtgggccttt ctgcgtttat atactagagg cccctcgctg agcgcgtccc ggagctgggg 961 gcaacctagc tgccacctgc ttttctgcta gctattccag cgaaaacata cagatttccg 1021 gcgaaatcaa ggctacctgc cagttctggc aggtttggcc gcgggttctt tttggtacac 1081 gaaagctact agagattaaa gaggagaaat actagatggc ttcctccgaa gacgttatca 1141 aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa 1201 tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta 1261 ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt 1321 ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg 1381 aaggtttcaa atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc 1441 aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact 1501 tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac 1561 gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag 1621 acggtggtca ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc 1681 tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca 1741 ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct 1801 gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc 1861 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 1921 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagttga cagctagctc 1981 agtcctaggt ataatgctag ctactagaga aagaggagaa atactagatg aggaatgacg 2041 gaggcttttt gctgtggtgg gacggtttgc gtagcgagat gcagccgatc cacgacagcc 2101 agggcgtgtt cgccgtcctg gaaaaggaag tgcggcgcct gggcttcgat tactacgcct 2161 atggcgtgcg ccacacgatt cccttcaccc ggccgaagac cgaggtccat ggcacctatc 2221 ccaaggcctg gctggagcga taccagatgc agaactacgg ggccgtggat ccggcgatcc 2281 tcaacggcct gcgctcctcg gaaatggtgg tctggagcga cagcctgttc gaccagagcc 2341 ggatgctctg gaacgaggct cgcgattggg gcctctgtgt cggcgcgacc ttgccgatcc 2401 gcgcgccgaa caatttgctc agcgtgcttt ccgtggcgcg cgaccagcag aacatctcca 2461 gcttcgagcg cgaggaaatc cgcctgcggc tgcgttgcat gatcgagttg ctgacccaga 2521 agctgaccga cctggagcat ccgatgctga tgtccaaccc ggtctgcctg agccatcgcg 2581 aacgcgagat cctgcaatgg accgccgacg gcaagagttc cggggaaatc gccatcatcc 2641 tgagcatctc cgagagcacg gtgaacttcc accacaagaa catccagaag aagttcgacg 2701 cgccgaacaa gacgctggct gccgcctacg ccgcggcgct gggtctcatc gctgcaaacg 2761 acgaaaacta cgctttagta gcttaataac actgatagtg ctagtgtaga tcactactag 2821 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 2881 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 2941 ctgcgtttat atactagagt cctgtgaaat ctggcagtta ccgttagctt tcgaattggc 3001 taaaaagtgt tctactagag attaaagagg agaaatacta gatggcttcc tccgaagacg 3061 ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt 3121 tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga 3181 aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt 3241 acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct 3301 tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg 3361 ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta 3421 ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca 3481 ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac 3541 tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg 3601 ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag 3661 actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat 3721 aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc 3781 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 3841 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //