##gff-version 3
##sequence-region BBa_K1407014 1 3826
BBa_K1407014	.	engineered_region	1967	3826	.	+	0	ID=BBa_K1407012;Name=BBa_K1407012
BBa_K1407014	.	engineered_region	2010	2893	.	+	0	ID=BBa_I1466;Name=BBa_I1466;Parent=BBa_K1407012
BBa_K1407014	.	terminator	2765	2844	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I1466
BBa_K1407014	.	engineered_region	2765	2844	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1407014	.	stem_loop	2776	2819	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1407014	.	terminator	2853	2893	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I1466
BBa_K1407014	.	stem_loop	2860	2879	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1407014	.	engineered_region	2853	2893	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1407014	.	polyA_site	2880	2893	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1407014	.	stop_codon	2886	2886	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1407014	.	ribosome_entry_site	2010	2021	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I1466
BBa_K1407014	.	conserved_region	2014	2017	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1407014	.	CDS	2028	2756	.	+	0	ID=BBa_C0171;Name=BBa_C0171;Parent=BBa_I1466
BBa_K1407014	.	promoter	1967	2001	.	+	0	ID=BBa_J23119;Name=BBa_J23119;Parent=BBa_K1407012
BBa_K1407014	.	engineered_region	2902	3689	.	+	0	ID=BBa_K575014;Name=BBa_K575014;Parent=BBa_K1407012
BBa_K1407014	.	promoter	2902	2954	.	+	0	ID=BBa_R0071;Name=BBa_R0071;Parent=BBa_K575014
BBa_K1407014	.	promoter	2943	2948	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_K1407014	.	start_codon	2954	2954	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_K1407014	.	promoter	2920	2925	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_K1407014	.	non_covalent_binding_site	2902	2921	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_K1407014	.	CDS	2984	3689	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K575014
BBa_K1407014	.	engineered_tag	3665	3689	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1407014	.	CDS	2984	3658	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1407014	.	ribosome_entry_site	2963	2977	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K575014
BBa_K1407014	.	ribosome_entry_site	2963	2977	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1407014	.	engineered_region	2963	2977	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1407014	.	ribosome_entry_site	2970	2974	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1407014	.	terminator	3698	3826	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1407012
BBa_K1407014	.	terminator	3698	3777	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1407014	.	engineered_region	3698	3777	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1407014	.	stem_loop	3709	3752	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1407014	.	terminator	3786	3826	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1407014	.	stem_loop	3793	3812	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1407014	.	engineered_region	3786	3826	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1407014	.	polyA_site	3813	3826	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1407014	.	stop_codon	3819	3819	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1407014	.	engineered_region	1	1958	.	+	0	ID=BBa_K1407010;Name=BBa_K1407010
BBa_K1407014	.	engineered_region	930	1821	.	+	0	ID=BBa_K575010;Name=BBa_K575010;Parent=BBa_K1407010
BBa_K1407014	.	ribosome_entry_site	1095	1109	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K575010
BBa_K1407014	.	ribosome_entry_site	1095	1109	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1407014	.	engineered_region	1095	1109	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1407014	.	ribosome_entry_site	1102	1106	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1407014	.	promoter	930	1086	.	+	0	ID=BBa_R0079;Name=BBa_R0079;Parent=BBa_K575010
BBa_K1407014	.	promoter	1069	1074	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_K1407014	.	non_covalent_binding_site	975	992	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_K1407014	.	non_covalent_binding_site	1035	1054	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_K1407014	.	promoter	1046	1051	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_K1407014	.	CDS	1116	1821	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K575010
BBa_K1407014	.	engineered_tag	1797	1821	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1407014	.	CDS	1116	1790	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1407014	.	terminator	1830	1958	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1407010
BBa_K1407014	.	terminator	1830	1909	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1407014	.	engineered_region	1830	1909	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1407014	.	stem_loop	1841	1884	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1407014	.	terminator	1918	1958	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1407014	.	stem_loop	1925	1944	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1407014	.	engineered_region	1918	1958	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1407014	.	polyA_site	1945	1958	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1407014	.	stop_codon	1951	1951	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1407014	.	engineered_region	1	921	.	+	0	ID=BBa_K553003;Name=BBa_K553003;Parent=BBa_K1407010
BBa_K1407014	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K553003
BBa_K1407014	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1407014	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K553003
BBa_K1407014	.	CDS	62	784	.	+	0	ID=BBa_C0179;Name=BBa_C0179;Parent=BBa_K553003
BBa_K1407014	.	terminator	793	921	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K553003
BBa_K1407014	.	terminator	793	872	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1407014	.	engineered_region	793	872	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1407014	.	stem_loop	804	847	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1407014	.	terminator	881	921	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1407014	.	stem_loop	888	907	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1407014	.	engineered_region	881	921	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1407014	.	polyA_site	908	921	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1407014	.	stop_codon	914	914	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_K1407014
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggccttggttgacggt
tttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattct
cgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgc
ctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccg
attttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgt
atgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggc
cgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccgga
ctggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcg
gcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcg
gaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataatactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcccctcgctgagcgcgtc
ccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaat
caaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctactagagattaaagaggag
aaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacg
gtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaa
aggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccg
gctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtg
gtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaactt
cccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggt
gctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctaca
tggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagacta
caccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtaga
tcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagct
agctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgaggaatgacggaggctttttgctg
tggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgc
ggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatgg
cacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctg
cgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggg
gcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagca
gaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgacc
gacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccg
acggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatcca
gaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataatactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcctgtgaaatctggcagttac
cgttagctttcgaattggctaaaaagtgttctactagagattaaagaggagaaatactagatggcttcctccgaagacg
ttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaagg
tgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggac
atcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgt
ccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctc
cctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaa
aaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtc
tgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccggg
tgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgct
gaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttata