LOCUS BBa_K1407015 3901 bp DNA linear 30-Apr-2024 DEFINITION LasR+LasR/PAI1 Inducible promoter+GFP+RhlR+RhlR/PAI2 Inducible promoter+RFP ACCESSION BBa_K1407015 VERSION BBa_K1407015.1 FEATURES Location/Qualifiers misc_feature 1..35 /label=BBa_J23100 misc_feature 1..921 /label=BBa_K553003 misc_feature 44..55 /label=BBa_B0034 misc_feature 48..51 /label=conserved misc_feature 62..784 /label=BBa_C0179 misc_feature 793..921 /label=BBa_B0015 misc_feature 793..872 /label=BBa_B0010 stem_loop 804..847 /label=stem_loop misc_feature 881..921 /label=BBa_B0012 stem_loop 888..907 /label=T7 TE polyA_site 908..921 /label=polya misc_feature 914..914 /label=stop misc_feature 930..1086 /label=BBa_R0079 misc_feature 930..1832 /label=BBa_K575009 misc_feature 975..992 /label=OP2 misc_feature 1035..1054 /label=OP1 promoter 1046..1051 /label=-35 promoter 1069..1074 /label=-10 misc_feature 1095..1106 /label=BBa_B0034 misc_feature 1099..1102 /label=conserved CDS 1113..1832 /label=GFP protein misc_feature 1113..1832 /label=BBa_E0040 misc_feature 1841..1920 /label=BBa_B0010 misc_feature 1841..1969 /label=BBa_B0015 stem_loop 1852..1895 /label=stem_loop misc_feature 1929..1969 /label=BBa_B0012 stem_loop 1936..1955 /label=T7 TE polyA_site 1956..1969 /label=polya misc_feature 1962..1962 /label=stop misc_feature 1..1969 /label=BBa_K1407008 misc_feature 1978..2012 /label=BBa_J23119 misc_feature 2021..2962 /label=BBa_I0466 misc_feature 2021..2032 /label=BBa_B0034 misc_feature 2025..2028 /label=conserved CDS 2039..2761 /label=rhlR misc_feature 2039..2800 /label=BBa_C0071 misc_feature 2762..2794 /label=LVA misc_feature 2801..2825 /label=Help:Barcodes misc_feature 2834..2913 /label=BBa_B0010 stem_loop 2845..2888 /label=stem_loop misc_feature 2922..2962 /label=BBa_B0012 stem_loop 2929..2948 /label=T7 TE polyA_site 2949..2962 /label=polya misc_feature 2955..2955 /label=stop misc_feature 2971..2990 /label=RhlR misc_feature 2971..3023 /label=BBa_R0071 misc_feature 2971..3758 /label=BBa_K575014 promoter 2989..2994 /label=-35 promoter 3012..3017 /label=-10 misc_feature 3023..3023 /label=start misc_feature 3032..3046 /label=BBa_B0030 RBS 3032..3046 /label=RBS-1\Strong RBS 3039..3043 /label=RBS CDS 3053..3727 /label=mrfp1 misc_feature 3053..3758 /label=BBa_E1010 misc_feature 3734..3758 /label=Help:Barcodes misc_feature 3767..3846 /label=BBa_B0010 misc_feature 3767..3895 /label=BBa_B0015 stem_loop 3778..3821 /label=stem_loop misc_feature 3855..3895 /label=BBa_B0012 stem_loop 3862..3881 /label=T7 TE polyA_site 3882..3895 /label=polya misc_feature 3888..3888 /label=stop misc_feature 1978..3895 /label=BBa_K1407011 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta 61 gatggccttg gttgacggtt ttcttgagct ggaacgctca agtggaaaat tggagtggag 121 cgccatcctc cagaagatgg cgagcgacct tggattctcg aagatcctgt tcggcctgtt 181 gcctaaggac agccaggact acgagaacgc cttcatcgtc ggcaactacc cggccgcctg 241 gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac ccgacggtca gtcactgtac 301 ccagagcgta ctgccgattt tctgggaacc gtccatctac cagacgcgaa agcagcacga 361 gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg ctgaccatgc cgctgcatgg 421 tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa gcggaaaacc gggccgaggc 481 caaccgtttc atagagtcgg tcctgccgac cctgtggatg ctcaaggact acgcactgca 541 aagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa ccggtggttc tgaccagccg 601 ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc agttgggaga tatcggttat 661 ctgcaactgc tcggaagcca atgtgaactt ccatatggga aatattcggc ggaagttcgg 721 tgtgacctcc cgccgcgtag cggccattat ggccgttaat ttgggtctta ttactctcta 781 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 841 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 901 gtgggccttt ctgcgtttat atactagagg cccctcgctg agcgcgtccc ggagctgggg 961 gcaacctagc tgccacctgc ttttctgcta gctattccag cgaaaacata cagatttccg 1021 gcgaaatcaa ggctacctgc cagttctggc aggtttggcc gcgggttctt tttggtacac 1081 gaaagctact agagaaagag gagaaatact agatgcgtaa aggagaagaa cttttcactg 1141 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca 1201 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta 1261 ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat 1321 gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg 1381 aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg 1441 ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt 1501 ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac tcacacaatg 1561 tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca 1621 acattgaaga tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg 1681 atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag 1741 atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta 1801 cacatggcat ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga 1861 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 1921 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagttg 1981 acagctagct cagtcctagg tataatgcta gctactagag aaagaggaga aatactagat 2041 gaggaatgac ggaggctttt tgctgtggtg ggacggtttg cgtagcgaga tgcagccgat 2101 ccacgacagc cagggcgtgt tcgccgtcct ggaaaaggaa gtgcggcgcc tgggcttcga 2161 ttactacgcc tatggcgtgc gccacacgat tcccttcacc cggccgaaga ccgaggtcca 2221 tggcacctat cccaaggcct ggctggagcg ataccagatg cagaactacg gggccgtgga 2281 tccggcgatc ctcaacggcc tgcgctcctc ggaaatggtg gtctggagcg acagcctgtt 2341 cgaccagagc cggatgctct ggaacgaggc tcgcgattgg ggcctctgtg tcggcgcgac 2401 cttgccgatc cgcgcgccga acaatttgct cagcgtgctt tccgtggcgc gcgaccagca 2461 gaacatctcc agcttcgagc gcgaggaaat ccgcctgcgg ctgcgttgca tgatcgagtt 2521 gctgacccag aagctgaccg acctggagca tccgatgctg atgtccaacc cggtctgcct 2581 gagccatcgc gaacgcgaga tcctgcaatg gaccgccgac ggcaagagtt ccggggaaat 2641 cgccatcatc ctgagcatct ccgagagcac ggtgaacttc caccacaaga acatccagaa 2701 gaagttcgac gcgccgaaca agacgctggc tgccgcctac gccgcggcgc tgggtctcat 2761 cgctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt gctagtgtag 2821 atcactacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 2881 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 2941 ggtgggcctt tctgcgttta tatactagag tcctgtgaaa tctggcagtt accgttagct 3001 ttcgaattgg ctaaaaagtg ttctactaga gattaaagag gagaaatact agatggcttc 3061 ctccgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa 3121 cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac 3181 cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc 3241 gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct 3301 gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg 3361 tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa 3421 actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg 3481 ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat 3541 gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc 3601 taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc 3661 ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac 3721 cggtgcttaa taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata 3781 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 3841 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta 3901 g //