##gff-version 3
##sequence-region BBa_K1407015 1 3895
BBa_K1407015	.	CDS	3053	3727	.	+	0	ID=annotation2413264;Name=mrfp1
BBa_K1407015	.	engineered_region	1	921	.	+	0	ID=annotation2413212;Name=BBa_K553003
BBa_K1407015	.	ribosome_entry_site	3039	3043	.	+	0	ID=annotation2413263;Name=RBS
BBa_K1407015	.	tag	2762	2794	.	+	0	ID=annotation2413247;Name=LVA
BBa_K1407015	.	polyA_site	3882	3895	.	+	0	ID=annotation2413272;Name=polya
BBa_K1407015	.	promoter	2989	2994	.	+	0	ID=annotation2413258;Name=-35
BBa_K1407015	.	engineered_region	2039	2800	.	+	0	ID=annotation2413246;Name=BBa_C0071
BBa_K1407015	.	CDS	2039	2761	.	+	0	ID=annotation2413245;Name=rhlR
BBa_K1407015	.	engineered_region	1095	1106	.	+	0	ID=annotation2413229;Name=BBa_B0034
BBa_K1407015	.	stop_codon	914	914	.	+	0	ID=annotation2413222;Name=stop
BBa_K1407015	.	engineered_region	2922	2962	.	+	0	ID=annotation2413251;Name=BBa_B0012
BBa_K1407015	.	polyA_site	1956	1969	.	+	0	ID=annotation2413238;Name=polya
BBa_K1407015	.	engineered_tag	2801	2825	.	+	0	ID=annotation2413248;Name=Help:Barcodes
BBa_K1407015	.	engineered_region	1113	1832	.	+	0	ID=annotation2413232;Name=BBa_E0040
BBa_K1407015	.	engineered_region	881	921	.	+	0	ID=annotation2413219;Name=BBa_B0012
BBa_K1407015	.	stem_loop	2845	2888	.	+	0	ID=annotation2413250;Name=stem_loop
BBa_K1407015	.	stem_loop	3862	3881	.	+	0	ID=annotation2413271;Name=T7 TE
BBa_K1407015	.	polyA_site	908	921	.	+	0	ID=annotation2413221;Name=polya
BBa_K1407015	.	non_covalent_binding_site	1035	1054	.	+	0	ID=annotation2413226;Name=OP1
BBa_K1407015	.	engineered_tag	3734	3758	.	+	0	ID=annotation2413266;Name=Help:Barcodes
BBa_K1407015	.	polyA_site	2949	2962	.	+	0	ID=annotation2413253;Name=polya
BBa_K1407015	.	engineered_region	2971	3023	.	+	0	ID=annotation2413256;Name=BBa_R0071
BBa_K1407015	.	conserved_region	48	51	.	+	0	ID=annotation2413214;Name=conserved
BBa_K1407015	.	stop_codon	3888	3888	.	+	0	ID=annotation2413273;Name=stop
BBa_K1407015	.	engineered_region	930	1086	.	+	0	ID=annotation2413223;Name=BBa_R0079
BBa_K1407015	.	engineered_region	793	872	.	+	0	ID=annotation2413217;Name=BBa_B0010
BBa_K1407015	.	ribosome_entry_site	3032	3046	.	+	0	ID=annotation2413262;Name=RBS-1\Strong
BBa_K1407015	.	stem_loop	1936	1955	.	+	0	ID=annotation2413237;Name=T7 TE
BBa_K1407015	.	engineered_region	793	921	.	+	0	ID=annotation2413216;Name=BBa_B0015
BBa_K1407015	.	conserved_region	2025	2028	.	+	0	ID=annotation2413244;Name=conserved
BBa_K1407015	.	stem_loop	888	907	.	+	0	ID=annotation2413220;Name=T7 TE
BBa_K1407015	.	engineered_region	1	1969	.	+	0	ID=annotation2413240;Name=BBa_K1407008
BBa_K1407015	.	engineered_region	1841	1969	.	+	0	ID=annotation2413234;Name=BBa_B0015
BBa_K1407015	.	engineered_region	2971	3758	.	+	0	ID=annotation2413257;Name=BBa_K575014
BBa_K1407015	.	engineered_region	3032	3046	.	+	0	ID=annotation2413261;Name=BBa_B0030
BBa_K1407015	.	stem_loop	804	847	.	+	0	ID=annotation2413218;Name=stem_loop
BBa_K1407015	.	non_covalent_binding_site	2971	2990	.	+	0	ID=annotation2413255;Name=RhlR
BBa_K1407015	.	engineered_region	1978	2012	.	+	0	ID=annotation2413241;Name=BBa_J23119
BBa_K1407015	.	engineered_region	930	1832	.	+	0	ID=annotation2413224;Name=BBa_K575009
BBa_K1407015	.	start_codon	3023	3023	.	+	0	ID=annotation2413260;Name=start
BBa_K1407015	.	stop_codon	1962	1962	.	+	0	ID=annotation2413239;Name=stop
BBa_K1407015	.	engineered_region	1978	3895	.	+	0	ID=annotation2413274;Name=BBa_K1407011
BBa_K1407015	.	promoter	3012	3017	.	+	0	ID=annotation2413259;Name=-10
BBa_K1407015	.	promoter	1069	1074	.	+	0	ID=annotation2413228;Name=-10
BBa_K1407015	.	stem_loop	3778	3821	.	+	0	ID=annotation2413269;Name=stem_loop
BBa_K1407015	.	CDS	1113	1832	.	+	0	ID=annotation2413231;Name=GFP protein
BBa_K1407015	.	stem_loop	1852	1895	.	+	0	ID=annotation2413235;Name=stem_loop
BBa_K1407015	.	engineered_region	2834	2913	.	+	0	ID=annotation2413249;Name=BBa_B0010
BBa_K1407015	.	engineered_region	44	55	.	+	0	ID=annotation2413213;Name=BBa_B0034
BBa_K1407015	.	engineered_region	2021	2962	.	+	0	ID=annotation2413242;Name=BBa_I0466
BBa_K1407015	.	engineered_region	1929	1969	.	+	0	ID=annotation2413236;Name=BBa_B0012
BBa_K1407015	.	engineered_region	3767	3895	.	+	0	ID=annotation2413268;Name=BBa_B0015
BBa_K1407015	.	engineered_region	62	784	.	+	0	ID=annotation2413215;Name=BBa_C0179
BBa_K1407015	.	engineered_region	3855	3895	.	+	0	ID=annotation2413270;Name=BBa_B0012
BBa_K1407015	.	engineered_region	1	35	.	+	0	ID=annotation2413211;Name=BBa_J23100
BBa_K1407015	.	stop_codon	2955	2955	.	+	0	ID=annotation2413254;Name=stop
BBa_K1407015	.	stem_loop	2929	2948	.	+	0	ID=annotation2413252;Name=T7 TE
BBa_K1407015	.	non_covalent_binding_site	975	992	.	+	0	ID=annotation2413225;Name=OP2
BBa_K1407015	.	engineered_region	1841	1920	.	+	0	ID=annotation2413233;Name=BBa_B0010
BBa_K1407015	.	conserved_region	1099	1102	.	+	0	ID=annotation2413230;Name=conserved
BBa_K1407015	.	engineered_region	3053	3758	.	+	0	ID=annotation2413265;Name=BBa_E1010
BBa_K1407015	.	promoter	1046	1051	.	+	0	ID=annotation2413227;Name=-35
BBa_K1407015	.	engineered_region	3767	3846	.	+	0	ID=annotation2413267;Name=BBa_B0010
BBa_K1407015	.	engineered_region	2021	2032	.	+	0	ID=annotation2413243;Name=BBa_B0034
>BBa_K1407015
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggccttggttgacggt
tttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattct
cgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgc
ctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccg
attttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgt
atgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggc
cgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccgga
ctggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcg
gcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcg
gaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataatactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcccctcgctgagcgcgtc
ccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaat
caaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctactagagaaagaggagaaa
tactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggc
acaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactgg
aaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcat
atgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacg
ggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattt
taaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaa
caaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatc
aacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaa
agatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaa
ctatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactag
agttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgaggaatgacggag
gctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctgga
aaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagacc
gaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcc
tcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggc
tcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcg
cgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgaccc
agaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgca
atggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccac
aagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatcgctg
caaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttatatactagagtcctgtgaaatctggcagttaccgttagcttt
cgaattggctaaaaagtgttctactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaaga
gttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgt
ccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccc
cgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccgga
aggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagac
ggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgg
gttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaa
agacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaa
accgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtc
actccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggct
cagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacctt
cgggtgggcctttctgcgtttatatactag