##gff-version 3
##sequence-region BBa_K1407016 1 3837
BBa_K1407016	.	stem_loop	1936	1955	.	+	0	ID=annotation2413162;Name=T7 TE
BBa_K1407016	.	conserved_region	2025	2028	.	+	0	ID=annotation2413169;Name=conserved
BBa_K1407016	.	non_covalent_binding_site	2913	2932	.	+	0	ID=annotation2413177;Name=RhlR
BBa_K1407016	.	conserved_region	48	51	.	+	0	ID=annotation2413139;Name=conserved
BBa_K1407016	.	engineered_region	1841	1920	.	+	0	ID=annotation2413158;Name=BBa_B0010
BBa_K1407016	.	polyA_site	2891	2904	.	+	0	ID=annotation2413175;Name=polya
BBa_K1407016	.	engineered_region	1978	2012	.	+	0	ID=annotation2413166;Name=BBa_J23119
BBa_K1407016	.	engineered_region	2974	2988	.	+	0	ID=annotation2413184;Name=BBa_B0030
BBa_K1407016	.	non_covalent_binding_site	975	992	.	+	0	ID=annotation2413150;Name=OP2
BBa_K1407016	.	engineered_region	1	35	.	+	0	ID=annotation2413136;Name=BBa_J23100
BBa_K1407016	.	engineered_region	2776	2855	.	+	0	ID=annotation2413171;Name=BBa_B0010
BBa_K1407016	.	stop_codon	2897	2897	.	+	0	ID=annotation2413176;Name=stop
BBa_K1407016	.	polyA_site	908	921	.	+	0	ID=annotation2413146;Name=polya
BBa_K1407016	.	engineered_region	3709	3837	.	+	0	ID=annotation2413190;Name=BBa_B0015
BBa_K1407016	.	engineered_region	1978	3837	.	+	0	ID=annotation2413196;Name=BBa_K1407012
BBa_K1407016	.	engineered_region	3709	3788	.	+	0	ID=annotation2413189;Name=BBa_B0010
BBa_K1407016	.	engineered_region	44	55	.	+	0	ID=annotation2413138;Name=BBa_B0034
BBa_K1407016	.	stem_loop	888	907	.	+	0	ID=annotation2413145;Name=T7 TE
BBa_K1407016	.	engineered_region	2913	2965	.	+	0	ID=annotation2413178;Name=BBa_R0071
BBa_K1407016	.	engineered_region	2021	2032	.	+	0	ID=annotation2413167;Name=BBa_B0034
BBa_K1407016	.	stem_loop	3804	3823	.	+	0	ID=annotation2413193;Name=T7 TE
BBa_K1407016	.	start_codon	2965	2965	.	+	0	ID=annotation2413182;Name=start
BBa_K1407016	.	engineered_region	2021	2904	.	+	0	ID=annotation2413168;Name=BBa_I1466
BBa_K1407016	.	stem_loop	3720	3763	.	+	0	ID=annotation2413191;Name=stem_loop
BBa_K1407016	.	engineered_region	2995	3700	.	+	0	ID=annotation2413187;Name=BBa_E1010
BBa_K1407016	.	engineered_region	793	921	.	+	0	ID=annotation2413141;Name=BBa_B0015
BBa_K1407016	.	engineered_region	2864	2904	.	+	0	ID=annotation2413173;Name=BBa_B0012
BBa_K1407016	.	engineered_region	881	921	.	+	0	ID=annotation2413144;Name=BBa_B0012
BBa_K1407016	.	engineered_region	62	784	.	+	0	ID=annotation2413140;Name=BBa_C0179
BBa_K1407016	.	stem_loop	804	847	.	+	0	ID=annotation2413143;Name=stem_loop
BBa_K1407016	.	polyA_site	1956	1969	.	+	0	ID=annotation2413163;Name=polya
BBa_K1407016	.	stop_codon	1962	1962	.	+	0	ID=annotation2413164;Name=stop
BBa_K1407016	.	stem_loop	2871	2890	.	+	0	ID=annotation2413174;Name=T7 TE
BBa_K1407016	.	engineered_region	930	1832	.	+	0	ID=annotation2413149;Name=BBa_K575009
BBa_K1407016	.	engineered_region	930	1086	.	+	0	ID=annotation2413148;Name=BBa_R0079
BBa_K1407016	.	engineered_region	1	921	.	+	0	ID=annotation2413137;Name=BBa_K553003
BBa_K1407016	.	promoter	1069	1074	.	+	0	ID=annotation2413153;Name=-10
BBa_K1407016	.	promoter	2954	2959	.	+	0	ID=annotation2413181;Name=-10
BBa_K1407016	.	CDS	2995	3669	.	+	0	ID=annotation2413186;Name=mrfp1
BBa_K1407016	.	ribosome_entry_site	2974	2988	.	+	0	ID=annotation2413183;Name=RBS-1\Strong
BBa_K1407016	.	engineered_region	1095	1106	.	+	0	ID=annotation2413154;Name=BBa_B0034
BBa_K1407016	.	polyA_site	3824	3837	.	+	0	ID=annotation2413194;Name=polya
BBa_K1407016	.	CDS	1113	1832	.	+	0	ID=annotation2413156;Name=GFP protein
BBa_K1407016	.	engineered_region	1113	1832	.	+	0	ID=annotation2413157;Name=BBa_E0040
BBa_K1407016	.	stop_codon	914	914	.	+	0	ID=annotation2413147;Name=stop
BBa_K1407016	.	engineered_region	2913	3700	.	+	0	ID=annotation2413179;Name=BBa_K575014
BBa_K1407016	.	ribosome_entry_site	2981	2985	.	+	0	ID=annotation2413185;Name=RBS
BBa_K1407016	.	stem_loop	2787	2830	.	+	0	ID=annotation2413172;Name=stem_loop
BBa_K1407016	.	engineered_region	2039	2767	.	+	0	ID=annotation2413170;Name=BBa_C0171
BBa_K1407016	.	engineered_tag	3676	3700	.	+	0	ID=annotation2413188;Name=Help:Barcodes
BBa_K1407016	.	engineered_region	793	872	.	+	0	ID=annotation2413142;Name=BBa_B0010
BBa_K1407016	.	promoter	2931	2936	.	+	0	ID=annotation2413180;Name=-35
BBa_K1407016	.	non_covalent_binding_site	1035	1054	.	+	0	ID=annotation2413151;Name=OP1
BBa_K1407016	.	engineered_region	1841	1969	.	+	0	ID=annotation2413159;Name=BBa_B0015
BBa_K1407016	.	conserved_region	1099	1102	.	+	0	ID=annotation2413155;Name=conserved
BBa_K1407016	.	stem_loop	1852	1895	.	+	0	ID=annotation2413160;Name=stem_loop
BBa_K1407016	.	engineered_region	1	1969	.	+	0	ID=annotation2413165;Name=BBa_K1407008
BBa_K1407016	.	stop_codon	3830	3830	.	+	0	ID=annotation2413195;Name=stop
BBa_K1407016	.	promoter	1046	1051	.	+	0	ID=annotation2413152;Name=-35
BBa_K1407016	.	engineered_region	1929	1969	.	+	0	ID=annotation2413161;Name=BBa_B0012
BBa_K1407016	.	engineered_region	3797	3837	.	+	0	ID=annotation2413192;Name=BBa_B0012
>BBa_K1407016
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggccttggttgacggt
tttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattct
cgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgc
ctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccg
attttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgt
atgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggc
cgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccgga
ctggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcg
gcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcg
gaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataatactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcccctcgctgagcgcgtc
ccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaat
caaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctactagagaaagaggagaaa
tactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggc
acaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactgg
aaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcat
atgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacg
ggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattt
taaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaa
caaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatc
aacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaa
agatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaa
ctatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactag
agttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgaggaatgacggag
gctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctgga
aaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagacc
gaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcc
tcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggc
tcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcg
cgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgaccc
agaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgca
atggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccac
aagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaat
aatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcctgtgaaat
ctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagattaaagaggagaaatactagatggcttc
ctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatc
gaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgt
tcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggacta
cctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacc
caggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccgg
ttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaat
caaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggtt
cagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagt
acgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttatatactag