BBa_K1407016
type: ComponentDefinition
persistent identity: BBa K1407016
display id: BBa_K1407016
version: 1
was generated by: iGEM to SBOL conversion
description: LasR+LasR/PAI1 Inducible promoter+GFP+RhlR(LVA-)+RhlR/PAI2 Inducible promoter+RFP
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:18Z
mutable provenance: Existing parts on the registry.
owned by: james
owned by: myers
mutable description: It's composed of seven sections:constitutive production of LasR,LasR/PAI1 Inducible promoter with RBS(B0034)and GFP,terminator,constitutive promoter,RhlR protein generator(LVA-) ,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,Terminator.When PAI1 exsits,the part could be inducibled and expresses GFP protein;When PAI2 exsits,the part could be inducibled and expresses RFP protein.
discontinued: false
dominant: false
group u list: _1785_
m user id: 0
owner id: 23052
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None.
star: false
creator: Ye Chen
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_K553003
sequence annotation: BBa_J23100
sequence annotation: BBa_K1407008
sequence annotation: BBa_B0034
sequence annotation: conserved
sequence annotation: BBa_C0179
sequence annotation: BBa_B0015
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence annotation: BBa_K575009
sequence annotation: BBa_R0079
sequence annotation: OP2
sequence annotation: OP1
sequence annotation: -35
sequence annotation: -10
sequence annotation: GFP protein
sequence annotation: BBa_E0040
sequence annotation: BBa_J23119
sequence annotation: BBa_K1407012
sequence annotation: BBa_I1466
sequence annotation: BBa_C0171
sequence annotation: RhlR
sequence annotation: BBa_R0071
sequence annotation: BBa_K575014
sequence annotation: start
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence annotation: mrfp1

BBa_K553003
type: SequenceAnnotation
persistent identity: annotation2413137
display id: annotation2413137
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413137/range2413137/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2413137
display id: range2413137
version: 1
top level: BBa_K1407016
direction: direction/reverse
start: 1
end: 921
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2413136
display id: annotation2413136
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413136/range2413136/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2413136
display id: range2413136
version: 1
top level: BBa_K1407016
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K1407008
type: SequenceAnnotation
persistent identity: annotation2413165
display id: annotation2413165
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413165/range2413165/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2413165
display id: range2413165
version: 1
top level: BBa_K1407016
direction: direction/reverse
start: 1
end: 1969
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2413138
display id: annotation2413138
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413138/range2413138/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2413138
display id: range2413138
version: 1
top level: BBa_K1407016
direction: direction/reverse
start: 44
end: 55
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2413139
display id: annotation2413139
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413139/range2413139/1
role: feature/conserved
role: SO:0000330

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type: Range
persistent identity: range2413139
display id: range2413139
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 48
end: 51
orientation: inline

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation2413140
display id: annotation2413140
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413140/range2413140/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2413140
display id: range2413140
version: 1
top level: BBa_K1407016
direction: direction/reverse
start: 62
end: 784
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2413141
display id: annotation2413141
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413141/range2413141/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2413141
display id: range2413141
version: 1
top level: BBa_K1407016
direction: direction/reverse
start: 793
end: 921
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2413142
display id: annotation2413142
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413142/range2413142/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2413142
display id: range2413142
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 793
end: 872
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2413143
display id: annotation2413143
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413143/range2413143/1
role: SO:0000313
role: feature/stem loop

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type: Range
persistent identity: range2413143
display id: range2413143
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 804
end: 847
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2413144
display id: annotation2413144
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413144/range2413144/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2413144
display id: range2413144
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 881
end: 921
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2413145
display id: annotation2413145
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413145/range2413145/1
role: feature/stem loop
role: SO:0000313

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type: Range
persistent identity: range2413145
display id: range2413145
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 888
end: 907
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2413146
display id: annotation2413146
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413146/range2413146/1
role: feature/polya
role: SO:0000553

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type: Range
persistent identity: range2413146
display id: range2413146
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 908
end: 921
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2413147
display id: annotation2413147
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413147/range2413147/1
role: feature/stop
role: SO:0000319

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type: Range
persistent identity: range2413147
display id: range2413147
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 914
end: 914
orientation: inline

BBa_K575009
type: SequenceAnnotation
persistent identity: annotation2413149
display id: annotation2413149
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413149/range2413149/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2413149
display id: range2413149
version: 1
top level: BBa_K1407016
direction: direction/reverse
start: 930
end: 1832
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2413148
display id: annotation2413148
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413148/range2413148/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2413148
display id: range2413148
version: 1
top level: BBa_K1407016
direction: direction/reverse
start: 930
end: 1086
orientation: inline

OP2
type: SequenceAnnotation
persistent identity: annotation2413150
display id: annotation2413150
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413150/range2413150/1
role: feature/binding
role: SO:0001091

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type: Range
persistent identity: range2413150
display id: range2413150
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 975
end: 992
orientation: inline

OP1
type: SequenceAnnotation
persistent identity: annotation2413151
display id: annotation2413151
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413151/range2413151/1
role: SO:0001091
role: feature/binding

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type: Range
persistent identity: range2413151
display id: range2413151
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 1035
end: 1054
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2413152
display id: annotation2413152
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413152/range2413152/1
role: feature/promoter
role: SO:0000167

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type: Range
persistent identity: range2413152
display id: range2413152
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 1046
end: 1051
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2413153
display id: annotation2413153
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413153/range2413153/1
role: SO:0000167
role: feature/promoter

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type: Range
persistent identity: range2413153
display id: range2413153
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 1069
end: 1074
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2413154
display id: annotation2413154
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413154/range2413154/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2413154
display id: range2413154
version: 1
top level: BBa_K1407016
direction: direction/reverse
start: 1095
end: 1106
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2413155
display id: annotation2413155
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413155/range2413155/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_K1407016/annotation2413155/range2413155/1
type: Range
persistent identity: range2413155
display id: range2413155
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 1099
end: 1102
orientation: inline

GFP protein
type: SequenceAnnotation
persistent identity: annotation2413156
display id: annotation2413156
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413156/range2413156/1
role: feature/protein
role: SO:0000316

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type: Range
persistent identity: range2413156
display id: range2413156
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 1113
end: 1832
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2413157
display id: annotation2413157
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413157/range2413157/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2413157
display id: range2413157
version: 1
top level: BBa_K1407016
direction: direction/reverse
start: 1113
end: 1832
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2413159
display id: annotation2413159
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413159/range2413159/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1407016/annotation2413159/range2413159/1
type: Range
persistent identity: range2413159
display id: range2413159
version: 1
top level: BBa_K1407016
direction: direction/reverse
start: 1841
end: 1969
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2413158
display id: annotation2413158
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413158/range2413158/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2413158
display id: range2413158
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 1841
end: 1920
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2413160
display id: annotation2413160
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413160/range2413160/1
role: SO:0000313
role: feature/stem loop

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type: Range
persistent identity: range2413160
display id: range2413160
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 1852
end: 1895
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2413161
display id: annotation2413161
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413161/range2413161/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2413161
display id: range2413161
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 1929
end: 1969
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2413162
display id: annotation2413162
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413162/range2413162/1
role: feature/stem loop
role: SO:0000313

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type: Range
persistent identity: range2413162
display id: range2413162
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 1936
end: 1955
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2413163
display id: annotation2413163
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413163/range2413163/1
role: SO:0000553
role: feature/polya

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type: Range
persistent identity: range2413163
display id: range2413163
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 1956
end: 1969
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2413164
display id: annotation2413164
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413164/range2413164/1
role: SO:0000319
role: feature/stop

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type: Range
persistent identity: range2413164
display id: range2413164
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 1962
end: 1962
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2413166
display id: annotation2413166
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413166/range2413166/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2413166
display id: range2413166
version: 1
top level: BBa_K1407016
direction: direction/reverse
start: 1978
end: 2012
orientation: inline

BBa_K1407012
type: SequenceAnnotation
persistent identity: annotation2413196
display id: annotation2413196
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413196/range2413196/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2413196
display id: range2413196
version: 1
top level: BBa_K1407016
direction: direction/reverse
start: 1978
end: 3837
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2413167
display id: annotation2413167
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413167/range2413167/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2413167
display id: range2413167
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 2021
end: 2032
orientation: inline

BBa_I1466
type: SequenceAnnotation
persistent identity: annotation2413168
display id: annotation2413168
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413168/range2413168/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2413168
display id: range2413168
version: 1
top level: BBa_K1407016
direction: direction/reverse
start: 2021
end: 2904
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2413169
display id: annotation2413169
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413169/range2413169/1
role: SO:0000330
role: feature/conserved

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type: Range
persistent identity: range2413169
display id: range2413169
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 2025
end: 2028
orientation: inline

BBa_C0171
type: SequenceAnnotation
persistent identity: annotation2413170
display id: annotation2413170
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413170/range2413170/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2413170
display id: range2413170
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 2039
end: 2767
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2413171
display id: annotation2413171
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413171/range2413171/1
role: feature/BioBrick
role: SO:0000804

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type: Range
persistent identity: range2413171
display id: range2413171
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 2776
end: 2855
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2413172
display id: annotation2413172
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413172/range2413172/1
role: feature/stem loop
role: SO:0000313

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type: Range
persistent identity: range2413172
display id: range2413172
version: 1
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direction: direction/forward
start: 2787
end: 2830
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2413173
display id: annotation2413173
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413173/range2413173/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1407016/annotation2413173/range2413173/1
type: Range
persistent identity: range2413173
display id: range2413173
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 2864
end: 2904
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2413174
display id: annotation2413174
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413174/range2413174/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_K1407016/annotation2413174/range2413174/1
type: Range
persistent identity: range2413174
display id: range2413174
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 2871
end: 2890
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2413175
display id: annotation2413175
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413175/range2413175/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_K1407016/annotation2413175/range2413175/1
type: Range
persistent identity: range2413175
display id: range2413175
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 2891
end: 2904
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2413176
display id: annotation2413176
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413176/range2413176/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1407016/annotation2413176/range2413176/1
type: Range
persistent identity: range2413176
display id: range2413176
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 2897
end: 2897
orientation: inline

RhlR
type: SequenceAnnotation
persistent identity: annotation2413177
display id: annotation2413177
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413177/range2413177/1
role: feature/binding
role: SO:0001091

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type: Range
persistent identity: range2413177
display id: range2413177
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 2913
end: 2932
orientation: inline

BBa_R0071
type: SequenceAnnotation
persistent identity: annotation2413178
display id: annotation2413178
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413178/range2413178/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1407016/annotation2413178/range2413178/1
type: Range
persistent identity: range2413178
display id: range2413178
version: 1
top level: BBa_K1407016
direction: direction/reverse
start: 2913
end: 2965
orientation: inline

BBa_K575014
type: SequenceAnnotation
persistent identity: annotation2413179
display id: annotation2413179
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413179/range2413179/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1407016/annotation2413179/range2413179/1
type: Range
persistent identity: range2413179
display id: range2413179
version: 1
top level: BBa_K1407016
direction: direction/reverse
start: 2913
end: 3700
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2413180
display id: annotation2413180
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413180/range2413180/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K1407016/annotation2413180/range2413180/1
type: Range
persistent identity: range2413180
display id: range2413180
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 2931
end: 2936
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2413181
display id: annotation2413181
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413181/range2413181/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1407016/annotation2413181/range2413181/1
type: Range
persistent identity: range2413181
display id: range2413181
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 2954
end: 2959
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2413182
display id: annotation2413182
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413182/range2413182/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K1407016/annotation2413182/range2413182/1
type: Range
persistent identity: range2413182
display id: range2413182
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 2965
end: 2965
orientation: inline

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation2413183
display id: annotation2413183
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413183/range2413183/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1407016/annotation2413183/range2413183/1
type: Range
persistent identity: range2413183
display id: range2413183
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 2974
end: 2988
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2413184
display id: annotation2413184
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413184/range2413184/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1407016/annotation2413184/range2413184/1
type: Range
persistent identity: range2413184
display id: range2413184
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 2974
end: 2988
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2413185
display id: annotation2413185
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413185/range2413185/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1407016/annotation2413185/range2413185/1
type: Range
persistent identity: range2413185
display id: range2413185
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 2981
end: 2985
orientation: inline

mrfp1
type: SequenceAnnotation
persistent identity: annotation2413186
display id: annotation2413186
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413186/range2413186/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1407016/annotation2413186/range2413186/1
type: Range
persistent identity: range2413186
display id: range2413186
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 2995
end: 3669
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2413187
display id: annotation2413187
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413187/range2413187/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1407016/annotation2413187/range2413187/1
type: Range
persistent identity: range2413187
display id: range2413187
version: 1
top level: BBa_K1407016
direction: direction/reverse
start: 2995
end: 3700
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2413188
display id: annotation2413188
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413188/range2413188/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_K1407016/annotation2413188/range2413188/1
type: Range
persistent identity: range2413188
display id: range2413188
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 3676
end: 3700
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2413189
display id: annotation2413189
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413189/range2413189/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1407016/annotation2413189/range2413189/1
type: Range
persistent identity: range2413189
display id: range2413189
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 3709
end: 3788
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2413190
display id: annotation2413190
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413190/range2413190/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1407016/annotation2413190/range2413190/1
type: Range
persistent identity: range2413190
display id: range2413190
version: 1
top level: BBa_K1407016
direction: direction/reverse
start: 3709
end: 3837
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2413191
display id: annotation2413191
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413191/range2413191/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K1407016/annotation2413191/range2413191/1
type: Range
persistent identity: range2413191
display id: range2413191
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 3720
end: 3763
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2413192
display id: annotation2413192
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413192/range2413192/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1407016/annotation2413192/range2413192/1
type: Range
persistent identity: range2413192
display id: range2413192
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 3797
end: 3837
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2413193
display id: annotation2413193
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413193/range2413193/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_K1407016/annotation2413193/range2413193/1
type: Range
persistent identity: range2413193
display id: range2413193
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 3804
end: 3823
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2413194
display id: annotation2413194
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413194/range2413194/1
role: feature/polya
role: SO:0000553

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type: Range
persistent identity: range2413194
display id: range2413194
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 3824
end: 3837
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2413195
display id: annotation2413195
version: 1
top level: BBa_K1407016
location: https://synbiohub.org/public/igem/BBa_K1407016/annotation2413195/range2413195/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1407016/annotation2413195/range2413195/1
type: Range
persistent identity: range2413195
display id: range2413195
version: 1
top level: BBa_K1407016
direction: direction/forward
start: 3830
end: 3830
orientation: inline

https://synbiohub.org/public/igem/BBa_K1407016_sequence/1
type: Sequence
persistent identity: BBa K1407016 sequence
display id: BBa_K1407016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctagg…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z