LOCUS BBa_K1407028 3891 bp DNA linear 22-Nov-2024 DEFINITION a bistable system ACCESSION BBa_K1407028 VERSION BBa_K1407028.1 FEATURES Location/Qualifiers promoter 1..251 /label=BBa_K1407099 CDS 258..1343 /label=BBa_I732100 RBS 1352..1366 /label=RBS-1\Strong RBS 1359..1363 /label=RBS misc_feature 1352..1366 /label=BBa_B0030 RBS 1352..1366 /label=BBa_B0030 CDS 1373..2092 /label=GFP protein CDS 1373..2092 /label=BBa_E0040 stem_loop 2112..2155 /label=stem_loop misc_feature 2101..2180 /label=BBa_B0010 terminator 2101..2180 /label=BBa_B0010 stem_loop 2196..2215 /label=T7 TE misc_feature 2222..2222 /label=stop polyA_site 2216..2229 /label=polya misc_feature 2189..2229 /label=BBa_B0012 terminator 2189..2229 /label=BBa_B0012 misc_feature 1352..2229 /label=BBa_E0840 CDS 2238..2325 /label=end of LacI coding region (inactive) misc_feature 2238..2437 /label=BBa_R0010 misc_feature 2326..2363 /label=CAP binding site promoter 2374..2379 /label=-35 promoter 2398..2403 /label=-10 misc_feature 2403..2437 /label=LacI binding site misc_feature 2410..2410 /label=start promoter 2238..2437 /label=BBa_R0010 CDS 2444..3055 /label=lexA CDS 2444..3055 /label=BBa_K864201 RBS 3064..3076 /label=RBS-3\Weak RBS 3070..3073 /label=RBS misc_feature 3064..3076 /label=BBa_B0032 RBS 3064..3076 /label=BBa_B0032 CDS 3083..3757 /label=mrfp1 misc_feature 3764..3788 /label=Help:Barcodes CDS 3083..3788 /label=BBa_E1010 stem_loop 3804..3823 /label=T7 TE misc_feature 3830..3830 /label=stop polyA_site 3824..3837 /label=polya misc_feature 3797..3837 /label=BBa_B0012 terminator 3797..3837 /label=BBa_B0012 stem_loop 3858..3880 /label=stem_loop misc_feature 3846..3891 /label=BBa_B0011 terminator 3846..3891 /label=BBa_B0011 terminator 3797..3891 /label=BBa_B0014 misc_feature 3064..3891 /label=BBa_K518012 ORIGIN 1 cggcagtgaa gagaagcctg tcggcaccgt ctggtttgct tttgccactg cccgcggtga 61 aggcattacc cggcgggaat gcttcagcgg cgaccgtgat gcggtgcgtc gtcaggctac 121 tgcgtatgca ttgcagacct tgtggcaaca atttctacaa aacacttgat actgtatgag 181 catacagtat aattgcttca acagaacata ttgactatcc ggtattaccc ggcatgacag 241 gagtaaccat gtactagatg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg 301 tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc 361 gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac 421 aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg 481 cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg 541 tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc 601 tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg 661 ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac 721 ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg 781 tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc 841 gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg 901 aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg 961 agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc 1021 gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg 1081 ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc 1141 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg 1201 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc 1261 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 1321 gactggaaag cgggcagtaa taatactaga gattaaagag gagaaatact agatgcgtaa 1381 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa 1441 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac 1501 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac 1561 tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt 1621 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga 1681 cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat 1741 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata 1801 caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt 1861 taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag accattatca 1921 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac 1981 acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt 2041 tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat aatactagag 2101 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 2161 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 2221 gcgtttatat actagagcaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 2281 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa 2341 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 2401 gttgtgtgga attgtgagcg gataacaatt tcacacatac tagatgaaag cgttaacggc 2461 caggcaacaa gaggtgtttg atctcatccg tgatcacatc agccagacag gtatgccgcc 2521 gacgcgtgcg gaaatcgcgc agcgtttggg gttccgttcc ccaaacgcgg ctgaagaaca 2581 tctgaaggcg ctggcacgca aaggcgttat tgaaattgtt tccggcgcat cacgcgggat 2641 tcgtctgttg caggaagagg aagaagggtt gccgctggta ggtcgtgtgg ctgccggtga 2701 accacttctg gcgcaacagc atattgaagg tcattatcag gtcgatcctt ccttattcaa 2761 gccgaatgct gatttcctgc tgcgcgtcag cgggatgtcg atgaaagata tcggcattat 2821 ggatggtgac ttgctggcag tgcataaaac tcaggatgta cgtaacggtc aggtcgttgt 2881 cgcacgtatt gatgacgaag ttaccgttaa gcgcctgaaa aaacagggca ataaagtcga 2941 actgttgcca gaaaatagcg agtttaaacc aattgtcgtt gaccttcgtc agcagagctt 3001 caccattgaa gggctggcgg ttggggttat tcgcaacggc gactggctgt aataatacta 3061 gagtcacaca ggaaagtact agatggcttc ctccgaagac gttatcaaag agttcatgcg 3121 tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg 3181 tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc 3241 gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt 3301 taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg 3361 ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg actcctccct 3421 gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgacgg 3481 tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga 3541 agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta 3601 cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta 3661 caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca tcgttgaaca 3721 gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taacgctgat agtgctagtg 3781 tagatcgcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac 3841 tagagagaga atataaaaag ccagattatt aatccggctt ttttattatt t //