##gff-version 3
##sequence-region BBa_K1407028 1 3891
BBa_K1407028	.	CDS	258	1343	.	+	0	ID=BBa_I732100;Name=BBa_I732100
BBa_K1407028	.	engineered_region	3064	3891	.	+	0	ID=BBa_K518012;Name=BBa_K518012
BBa_K1407028	.	ribosome_entry_site	3064	3076	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K518012
BBa_K1407028	.	engineered_region	3064	3076	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1407028	.	ribosome_entry_site	3064	3076	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1407028	.	ribosome_entry_site	3070	3073	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1407028	.	terminator	3797	3891	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K518012
BBa_K1407028	.	terminator	3846	3891	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K1407028	.	stem_loop	3858	3880	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K1407028	.	engineered_region	3846	3891	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K1407028	.	terminator	3797	3837	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K1407028	.	polyA_site	3824	3837	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1407028	.	engineered_region	3797	3837	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1407028	.	stem_loop	3804	3823	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1407028	.	stop_codon	3830	3830	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1407028	.	CDS	3083	3788	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K518012
BBa_K1407028	.	CDS	3083	3757	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1407028	.	engineered_tag	3764	3788	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1407028	.	engineered_region	1352	2229	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_K1407028	.	terminator	2189	2229	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K1407028	.	polyA_site	2216	2229	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1407028	.	engineered_region	2189	2229	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1407028	.	stem_loop	2196	2215	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1407028	.	stop_codon	2222	2222	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1407028	.	ribosome_entry_site	1352	1366	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K1407028	.	ribosome_entry_site	1359	1363	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1407028	.	ribosome_entry_site	1352	1366	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1407028	.	engineered_region	1352	1366	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1407028	.	CDS	1373	2092	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K1407028	.	CDS	1373	2092	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1407028	.	terminator	2101	2180	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K1407028	.	stem_loop	2112	2155	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1407028	.	engineered_region	2101	2180	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1407028	.	CDS	2444	3055	.	+	0	ID=BBa_K864201;Name=BBa_K864201
BBa_K1407028	.	CDS	2444	3055	.	+	0	ID=annotation2197113;Name=lexA;Parent=BBa_K864201
BBa_K1407028	.	promoter	2238	2437	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K1407028	.	start_codon	2410	2410	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1407028	.	CDS	2238	2325	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1407028	.	promoter	2398	2403	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1407028	.	non_covalent_binding_site	2403	2437	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1407028	.	non_covalent_binding_site	2326	2363	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1407028	.	engineered_region	2238	2437	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1407028	.	promoter	2374	2379	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1407028	.	promoter	1	251	.	+	0	ID=BBa_K1407099;Name=BBa_K1407099
>BBa_K1407028
cggcagtgaagagaagcctgtcggcaccgtctggtttgcttttgccactgcccgcggtgaaggcattacccggcgggaa
tgcttcagcggcgaccgtgatgcggtgcgtcgtcaggctactgcgtatgcattgcagaccttgtggcaacaatttctac
aaaacacttgatactgtatgagcatacagtataattgcttcaacagaacatattgactatccggtattacccggcatga
caggagtaaccatgtactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgt
ttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaat
tacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccc
tgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggt
agaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaac
tatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctg
accagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattggg
tcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatat
ctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgc
aaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccat
taccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatc
ccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagg
gccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaac
cgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataa
tactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttga
attagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacc
cttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaat
gctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaag
aactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaataga
atcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacaca
atgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaag
cgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctg
tccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctg
ggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggc
acgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcacccca
ggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagatgaaa
gcgttaacggccaggcaacaagaggtgtttgatctcatccgtgatcacatcagccagacaggtatgccgccgacgcgtg
cggaaatcgcgcagcgtttggggttccgttccccaaacgcggctgaagaacatctgaaggcgctggcacgcaaaggcgt
tattgaaattgtttccggcgcatcacgcgggattcgtctgttgcaggaagaggaagaagggttgccgctggtaggtcgt
gtggctgccggtgaaccacttctggcgcaacagcatattgaaggtcattatcaggtcgatccttccttattcaagccga
atgctgatttcctgctgcgcgtcagcgggatgtcgatgaaagatatcggcattatggatggtgacttgctggcagtgca
taaaactcaggatgtacgtaacggtcaggtcgttgtcgcacgtattgatgacgaagttaccgttaagcgcctgaaaaaa
cagggcaataaagtcgaactgttgccagaaaatagcgagtttaaaccaattgtcgttgaccttcgtcagcagagcttca
ccattgaagggctggcggttggggttattcgcaacggcgactggctgtaataatactagagtcacacaggaaagtacta
gatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgag
ttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtc
cgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacat
cccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgtt
accgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccg
acggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaa
aggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaa
aaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcg
ttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattatta
atccggcttttttattattt