LOCUS       BBa_K1407035            1654 bp    DNA     linear       22-Dec-2024
DEFINITION  a part of bistable system
ACCESSION   BBa_K1407035
VERSION     BBa_K1407035.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     CDS             207..818
                     /label=lexA
     CDS             207..818
                     /label=BBa_K864201
     RBS             827..839
                     /label=RBS-3\Weak
     RBS             833..836
                     /label=RBS
     misc_feature    827..839
                     /label=BBa_B0032
     RBS             827..839
                     /label=BBa_B0032
     CDS             846..1520
                     /label=mrfp1
     misc_feature    1527..1551
                     /label=Help:Barcodes
     CDS             846..1551
                     /label=BBa_E1010
     stem_loop       1567..1586
                     /label=T7 TE
     misc_feature    1593..1593
                     /label=stop
     polyA_site      1587..1600
                     /label=polya
     misc_feature    1560..1600
                     /label=BBa_B0012
     terminator      1560..1600
                     /label=BBa_B0012
     stem_loop       1621..1643
                     /label=stem_loop
     misc_feature    1609..1654
                     /label=BBa_B0011
     terminator      1609..1654
                     /label=BBa_B0011
     terminator      1560..1654
                     /label=BBa_B0014
     misc_feature    827..1654
                     /label=BBa_K518012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagatga aagcgttaac ggccaggcaa caagaggtgt
      241 ttgatctcat ccgtgatcac atcagccaga caggtatgcc gccgacgcgt gcggaaatcg
      301 cgcagcgttt ggggttccgt tccccaaacg cggctgaaga acatctgaag gcgctggcac
      361 gcaaaggcgt tattgaaatt gtttccggcg catcacgcgg gattcgtctg ttgcaggaag
      421 aggaagaagg gttgccgctg gtaggtcgtg tggctgccgg tgaaccactt ctggcgcaac
      481 agcatattga aggtcattat caggtcgatc cttccttatt caagccgaat gctgatttcc
      541 tgctgcgcgt cagcgggatg tcgatgaaag atatcggcat tatggatggt gacttgctgg
      601 cagtgcataa aactcaggat gtacgtaacg gtcaggtcgt tgtcgcacgt attgatgacg
      661 aagttaccgt taagcgcctg aaaaaacagg gcaataaagt cgaactgttg ccagaaaata
      721 gcgagtttaa accaattgtc gttgaccttc gtcagcagag cttcaccatt gaagggctgg
      781 cggttggggt tattcgcaac ggcgactggc tgtaataata ctagagtcac acaggaaagt
      841 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg
      901 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg
      961 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg
     1021 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca
     1081 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact
     1141 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca
     1201 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa
     1261 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag
     1321 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa
     1381 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac
     1441 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag
     1501 gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagt
     1561 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagag agaatataaa
     1621 aagccagatt attaatccgg cttttttatt attt
//