LOCUS BBa_K1407036 2850 bp DNA linear 22-Nov-2024 DEFINITION Lux pL controlled LuxR with lux pR autoinducing LuxI(lva tag)- AHL-GFP ACCESSION BBa_K1407036 VERSION BBa_K1407036.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 164..167 /label=conserved RBS 160..171 /label=BBa_B0034 misc_feature 178..179 /label=prefix misc_feature 351..351 /label=T misc_feature 669..669 /label=A CDS 178..927 /label=luxR misc_feature 178..933 /label=BBa_C0062 misc_feature 934..958 /label=Help:Barcodes CDS 178..933 /label=BBa_C0062 stem_loop 978..1021 /label=stem_loop misc_feature 967..1046 /label=BBa_B0010 terminator 967..1046 /label=BBa_B0010 stem_loop 1062..1081 /label=T7 TE misc_feature 1088..1088 /label=stop polyA_site 1082..1095 /label=polya misc_feature 1055..1095 /label=BBa_B0012 terminator 1055..1095 /label=BBa_B0012 misc_feature 1104..1123 /label=LuxR/HSL promoter 1123..1128 /label=-35 promoter 1145..1150 /label=-10 misc_feature 1156..1156 /label=start misc_feature 1104..1158 /label=BBa_R0062 promoter 1104..1158 /label=BBa_R0062 misc_feature 1..1158 /label=BBa_F2621 misc_feature 1171..1174 /label=conserved RBS 1167..1178 /label=BBa_B0034 misc_feature 1764..1795 /label=LVA CDS 1185..1763 /label=luxI misc_feature 1185..1802 /label=BBa_C0061 misc_feature 1803..1827 /label=Help:Barcodes CDS 1185..1802 /label=BBa_C0061 misc_feature 1167..1827 /label=BBa_C0261 stem_loop 1847..1890 /label=stem_loop misc_feature 1836..1915 /label=BBa_B0010 terminator 1836..1915 /label=BBa_B0010 stem_loop 1931..1950 /label=T7 TE misc_feature 1957..1957 /label=stop polyA_site 1951..1964 /label=polya misc_feature 1924..1964 /label=BBa_B0012 terminator 1924..1964 /label=BBa_B0012 terminator 1836..1964 /label=BBa_B0015 misc_feature 1167..1964 /label=BBa_K082014 misc_feature 1..1964 /label=BBa_K546000 RBS 1973..1987 /label=RBS-1\Strong RBS 1980..1984 /label=RBS misc_feature 1973..1987 /label=BBa_B0030 RBS 1973..1987 /label=BBa_B0030 CDS 1994..2713 /label=GFP protein CDS 1994..2713 /label=BBa_E0040 stem_loop 2733..2776 /label=stem_loop misc_feature 2722..2801 /label=BBa_B0010 terminator 2722..2801 /label=BBa_B0010 stem_loop 2817..2836 /label=T7 TE misc_feature 2843..2843 /label=stop polyA_site 2837..2850 /label=polya misc_feature 2810..2850 /label=BBa_B0012 terminator 2810..2850 /label=BBa_B0012 misc_feature 1973..2850 /label=BBa_E0840 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg 181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt 1141 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatgact ataatgataa 1201 aaaaatcgga ttttttggca attccatcgg aggagtataa aggtattcta agtcttcgtt 1261 atcaagtgtt taagcaaaga cttgagtggg acttagttgt agaaaataac cttgaatcag 1321 atgagtatga taactcaaat gcagaatata tttatgcttg tgatgatact gaaaatgtaa 1381 gtggatgctg gcgtttatta cctacaacag gtgattatat gctgaaaagt gtttttcctg 1441 aattgcttgg tcaacagagt gctcccaaag atcctaatat agtcgaatta agtcgttttg 1501 ctgtaggtaa aaatagctca aagataaata actctgctag tgaaattaca atgaaactat 1561 ttgaagctat atataaacac gctgttagtc aaggtattac agaatatgta acagtaacat 1621 caacagcaat agagcgattt ttaaagcgta ttaaagttcc ttgtcatcgt attggagaca 1681 aagaaattca tgtattaggt gatactaaat cggttgtatt gtctatgcct attaatgaac 1741 agtttaaaaa agcagtctta aatgctgcaa acgacgaaaa ctacgcttta gtagcttaat 1801 aactctgata gtgctagtgt agatctctac tagagccagg catcaaataa aacgaaaggc 1861 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 1921 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agattaaaga 1981 ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt 2041 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga 2101 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc 2161 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga 2221 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag 2281 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg 2341 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat 2401 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa 2461 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt 2521 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc 2581 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga 2641 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact 2701 atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 2761 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 2821 caccttcggg tgggcctttc tgcgtttata //