LOCUS       BBa_K1412000            1008 bp    DNA     linear       22-Dec-2024
DEFINITION  CheZ generator under Lac promoter
ACCESSION   BBa_K1412000
VERSION     BBa_K1412000.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     primer_bind     227..246
                     /label=fwd primer
     misc_feature    227..229
                     /label=start
     primer_bind     850..871
                     /label=rev primer
     misc_feature    869..871
                     /label=terminator
     CDS             227..871
                     /label=CheZ
     misc_feature    869..871
                     /label=TGA to TAA
     CDS             227..871
                     /label=BBa_K629003
     stem_loop       891..934
                     /label=stem_loop
     misc_feature    880..959
                     /label=BBa_B0010
     terminator      880..959
                     /label=BBa_B0010
     stem_loop       975..994
                     /label=T7 TE
     misc_feature    1001..1001
                     /label=stop
     polyA_site      995..1008
                     /label=polya
     misc_feature    968..1008
                     /label=BBa_B0012
     terminator      968..1008
                     /label=BBa_B0012
     terminator      880..1008
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga tgcaaccatc
      241 aatcaaacct gctgacgagc attcagctgg cgatatcatt gcgcgcatcg gcagcctgac
      301 gcgtatgctg cgcgacagtt tgcgggaact ggggctggat caggccattg ccgaagcggc
      361 ggaagccatc cccgatgcgc gcgatcgttt gtactatgtt gtgcagatga ccgcccaggc
      421 tgcggagcgg gcgctgaaca gtgttgaggc gtcacaaccg catcaggatc aaatggagaa
      481 atcagcaaaa gcgttaaccc aacgttggga tgactggttt gccgatccga ttgaccttgc
      541 cgacgcccgt gaactggtaa cagatacacg acaatttctg gcagatgtac ccgcgcatac
      601 cagctttact aacgcgcaac tgctggaaat catgatggcg caggattttc aggatctcac
      661 cgggcaggtc attaagcgga tgatggatgt cattcaggag atcgaacgcc agttgctgat
      721 ggtgctgttg gaaaacatcc cggaacagga gtcgcgtcca aaacgtgaaa accagagttt
      781 gcttaatgga cctcaggtcg ataccagcaa agccggtgtg gtagccagtc aggatcaggt
      841 ggacgatttg ttggatagtc ttggatttta atactagagc caggcatcaa ataaaacgaa
      901 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
      961 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//