LOCUS       BBa_K1412001            2395 bp    DNA     linear       22-Nov-2024
DEFINITION  Endow the E.coli engineered strain(CL-1) the ability of chemotaxis
ACCESSION   BBa_K1412001
VERSION     BBa_K1412001.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206000
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     CDS             157..1242
                     /label=BBa_I732100
     stem_loop       1262..1305
                     /label=stem_loop
     misc_feature    1251..1330
                     /label=BBa_B0010
     terminator      1251..1330
                     /label=BBa_B0010
     stem_loop       1346..1365
                     /label=T7 TE
     misc_feature    1372..1372
                     /label=stop
     polyA_site      1366..1379
                     /label=polya
     misc_feature    1339..1379
                     /label=BBa_B0012
     terminator      1339..1379
                     /label=BBa_B0012
     misc_feature    139..1379
                     /label=BBa_I732820
     CDS             1388..1475
                     /label=end of LacI coding region (inactive)
     misc_feature    1388..1587
                     /label=BBa_R0010
     misc_feature    1476..1513
                     /label=CAP binding site
     promoter        1524..1529
                     /label=-35
     promoter        1548..1553
                     /label=-10
     misc_feature    1553..1587
                     /label=LacI binding site
     misc_feature    1560..1560
                     /label=start
     promoter        1388..1587
                     /label=BBa_R0010
     misc_feature    1600..1603
                     /label=conserved
     RBS             1596..1607
                     /label=BBa_B0034
     primer_bind     1614..1633
                     /label=fwd primer
     misc_feature    1614..1616
                     /label=start
     primer_bind     2237..2258
                     /label=rev primer
     misc_feature    2256..2258
                     /label=terminator
     CDS             1614..2258
                     /label=CheZ
     misc_feature    2256..2258
                     /label=TGA to TAA
     CDS             1614..2258
                     /label=BBa_K629003
     stem_loop       2278..2321
                     /label=stem_loop
     misc_feature    2267..2346
                     /label=BBa_B0010
     terminator      2267..2346
                     /label=BBa_B0010
     stem_loop       2362..2381
                     /label=T7 TE
     misc_feature    2388..2388
                     /label=stop
     polyA_site      2382..2395
                     /label=polya
     misc_feature    2355..2395
                     /label=BBa_B0012
     terminator      2355..2395
                     /label=BBa_B0012
     terminator      2267..2395
                     /label=BBa_B0015
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatga aaccagtaac gttatacgat
      181 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc
      241 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt
      301 cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc
      361 tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat
      421 caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa
      481 gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg
      541 gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt
      601 gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga
      661 ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca
      721 ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat
      781 caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa
      841 accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag
      901 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc
      961 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc
     1021 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct
     1081 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc
     1141 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1201 ctggcacgac aggtttcccg actggaaagc gggcagtaat aatactagag ccaggcatca
     1261 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     1321 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
     1381 actagagcaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     1441 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     1501 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     1561 attgtgagcg gataacaatt tcacacatac tagagaaaga ggagaaatac tagatgatgc
     1621 aaccatcaat caaacctgct gacgagcatt cagctggcga tatcattgcg cgcatcggca
     1681 gcctgacgcg tatgctgcgc gacagtttgc gggaactggg gctggatcag gccattgccg
     1741 aagcggcgga agccatcccc gatgcgcgcg atcgtttgta ctatgttgtg cagatgaccg
     1801 cccaggctgc ggagcgggcg ctgaacagtg ttgaggcgtc acaaccgcat caggatcaaa
     1861 tggagaaatc agcaaaagcg ttaacccaac gttgggatga ctggtttgcc gatccgattg
     1921 accttgccga cgcccgtgaa ctggtaacag atacacgaca atttctggca gatgtacccg
     1981 cgcataccag ctttactaac gcgcaactgc tggaaatcat gatggcgcag gattttcagg
     2041 atctcaccgg gcaggtcatt aagcggatga tggatgtcat tcaggagatc gaacgccagt
     2101 tgctgatggt gctgttggaa aacatcccgg aacaggagtc gcgtccaaaa cgtgaaaacc
     2161 agagtttgct taatggacct caggtcgata ccagcaaagc cggtgtggta gccagtcagg
     2221 atcaggtgga cgatttgttg gatagtcttg gattttaata ctagagccag gcatcaaata
     2281 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     2341 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//