##gff-version 3
##sequence-region BBa_K1412001 1 2395
BBa_K1412001	.	engineered_region	139	1379	.	+	0	ID=BBa_I732820;Name=BBa_I732820
BBa_K1412001	.	terminator	1339	1379	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I732820
BBa_K1412001	.	polyA_site	1366	1379	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1412001	.	engineered_region	1339	1379	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1412001	.	stem_loop	1346	1365	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1412001	.	stop_codon	1372	1372	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1412001	.	CDS	157	1242	.	+	0	ID=BBa_I732100;Name=BBa_I732100;Parent=BBa_I732820
BBa_K1412001	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732820
BBa_K1412001	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1412001	.	terminator	1251	1330	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I732820
BBa_K1412001	.	stem_loop	1262	1305	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1412001	.	engineered_region	1251	1330	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1412001	.	ribosome_entry_site	1596	1607	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1412001	.	conserved_region	1600	1603	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1412001	.	CDS	1614	2258	.	+	0	ID=BBa_K629003;Name=BBa_K629003
BBa_K1412001	.	stop_codon	2256	2258	.	+	0	ID=annotation2154424;Name=terminator;Parent=BBa_K629003
BBa_K1412001	.	primer_binding_site	1614	1633	.	+	0	ID=annotation2154421;Name=fwd primer;Parent=BBa_K629003
BBa_K1412001	.	CDS	1614	2258	.	+	0	ID=annotation2154425;Name=CheZ;Parent=BBa_K629003
BBa_K1412001	.	sequence_alteration	2256	2258	.	+	0	ID=annotation2155668;Name=TGA to TAA;Parent=BBa_K629003
BBa_K1412001	.	start_codon	1614	1616	.	+	0	ID=annotation2154422;Name=start;Parent=BBa_K629003
BBa_K1412001	.	primer_binding_site	2237	2258	.	+	0	ID=annotation2154423;Name=rev primer;Parent=BBa_K629003
BBa_K1412001	.	terminator	2267	2395	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1412001	.	terminator	2355	2395	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1412001	.	polyA_site	2382	2395	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1412001	.	engineered_region	2355	2395	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1412001	.	stem_loop	2362	2381	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1412001	.	stop_codon	2388	2388	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1412001	.	terminator	2267	2346	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1412001	.	stem_loop	2278	2321	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1412001	.	engineered_region	2267	2346	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1412001	.	promoter	1	130	.	+	0	ID=BBa_K206000;Name=BBa_K206000
BBa_K1412001	.	promoter	1	131	.	+	0	ID=annotation2049252;Name=promoter;Parent=BBa_K206000
BBa_K1412001	.	operator	40	57	.	+	0	ID=annotation2049253;Name=AraI1;Parent=BBa_K206000
BBa_K1412001	.	operator	61	78	.	+	0	ID=annotation2049254;Name=AraI2;Parent=BBa_K206000
BBa_K1412001	.	promoter	1388	1587	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K1412001	.	start_codon	1560	1560	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1412001	.	CDS	1388	1475	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1412001	.	promoter	1548	1553	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1412001	.	non_covalent_binding_site	1553	1587	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1412001	.	non_covalent_binding_site	1476	1513	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1412001	.	engineered_region	1388	1587	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1412001	.	promoter	1524	1529	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
>BBa_K1412001
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
gaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggcc
agccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcac
aacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgt
cgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcc
tgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatg
ccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtat
tattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgtta
gcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagc
cgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgt
tcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgtt
ggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaac
aggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatca
gctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggcc
gattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataatactagagccaggcatcaaata
aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccg
attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagc
tcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatt
tcacacatactagagaaagaggagaaatactagatgatgcaaccatcaatcaaacctgctgacgagcattcagctggcg
atatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgc
cgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgg
gcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttggg
atgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgt
acccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcag
gtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaac
aggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagc
cagtcaggatcaggtggacgatttgttggatagtcttggattttaatactagagccaggcatcaaataaaacgaaaggc
tcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacct
tcgggtgggcctttctgcgtttata