LOCUS       BBa_K1412002            2257 bp    DNA     linear       22-Dec-2024
DEFINITION  Control group of K1412001
ACCESSION   BBa_K1412002
VERSION     BBa_K1412002.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..1104
                     /label=BBa_I732100
     stem_loop       1124..1167
                     /label=stem_loop
     misc_feature    1113..1192
                     /label=BBa_B0010
     terminator      1113..1192
                     /label=BBa_B0010
     stem_loop       1208..1227
                     /label=T7 TE
     misc_feature    1234..1234
                     /label=stop
     polyA_site      1228..1241
                     /label=polya
     misc_feature    1201..1241
                     /label=BBa_B0012
     terminator      1201..1241
                     /label=BBa_B0012
     misc_feature    1..1241
                     /label=BBa_I732820
     CDS             1250..1337
                     /label=end of LacI coding region (inactive)
     misc_feature    1250..1449
                     /label=BBa_R0010
     misc_feature    1338..1375
                     /label=CAP binding site
     promoter        1386..1391
                     /label=-35
     promoter        1410..1415
                     /label=-10
     misc_feature    1415..1449
                     /label=LacI binding site
     misc_feature    1422..1422
                     /label=start
     promoter        1250..1449
                     /label=BBa_R0010
     misc_feature    1462..1465
                     /label=conserved
     RBS             1458..1469
                     /label=BBa_B0034
     primer_bind     1476..1495
                     /label=fwd primer
     misc_feature    1476..1478
                     /label=start
     primer_bind     2099..2120
                     /label=rev primer
     misc_feature    2118..2120
                     /label=terminator
     CDS             1476..2120
                     /label=CheZ
     misc_feature    2118..2120
                     /label=TGA to TAA
     CDS             1476..2120
                     /label=BBa_K629003
     stem_loop       2140..2183
                     /label=stem_loop
     misc_feature    2129..2208
                     /label=BBa_B0010
     terminator      2129..2208
                     /label=BBa_B0010
     stem_loop       2224..2243
                     /label=T7 TE
     misc_feature    2250..2250
                     /label=stop
     polyA_site      2244..2257
                     /label=polya
     misc_feature    2217..2257
                     /label=BBa_B0012
     terminator      2217..2257
                     /label=BBa_B0012
     terminator      2129..2257
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagat gaaaccagta acgttatacg atgtcgcaga gtatgccggt
       61 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg
      121 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa
      181 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac
      241 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg
      301 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt
      361 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt
      421 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca
      481 cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg
      541 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg
      601 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg
      661 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat
      721 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg
      781 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac
      841 gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc
      901 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag
      961 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg
     1021 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     1081 cgactggaaa gcgggcagta ataatactag agccaggcat caaataaaac gaaaggctca
     1141 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     1201 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagc aatacgcaaa
     1261 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     1321 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     1381 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     1441 tttcacacat actagagaaa gaggagaaat actagatgat gcaaccatca atcaaacctg
     1501 ctgacgagca ttcagctggc gatatcattg cgcgcatcgg cagcctgacg cgtatgctgc
     1561 gcgacagttt gcgggaactg gggctggatc aggccattgc cgaagcggcg gaagccatcc
     1621 ccgatgcgcg cgatcgtttg tactatgttg tgcagatgac cgcccaggct gcggagcggg
     1681 cgctgaacag tgttgaggcg tcacaaccgc atcaggatca aatggagaaa tcagcaaaag
     1741 cgttaaccca acgttgggat gactggtttg ccgatccgat tgaccttgcc gacgcccgtg
     1801 aactggtaac agatacacga caatttctgg cagatgtacc cgcgcatacc agctttacta
     1861 acgcgcaact gctggaaatc atgatggcgc aggattttca ggatctcacc gggcaggtca
     1921 ttaagcggat gatggatgtc attcaggaga tcgaacgcca gttgctgatg gtgctgttgg
     1981 aaaacatccc ggaacaggag tcgcgtccaa aacgtgaaaa ccagagtttg cttaatggac
     2041 ctcaggtcga taccagcaaa gccggtgtgg tagccagtca ggatcaggtg gacgatttgt
     2101 tggatagtct tggattttaa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     2161 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     2221 actggctcac cttcgggtgg gcctttctgc gtttata
//