##gff-version 3
##sequence-region BBa_K1412002 1 2257
BBa_K1412002	.	CDS	1476	2120	.	+	0	ID=BBa_K629003;Name=BBa_K629003
BBa_K1412002	.	start_codon	1476	1478	.	+	0	ID=annotation2154422;Name=start;Parent=BBa_K629003
BBa_K1412002	.	primer_binding_site	1476	1495	.	+	0	ID=annotation2154421;Name=fwd primer;Parent=BBa_K629003
BBa_K1412002	.	primer_binding_site	2099	2120	.	+	0	ID=annotation2154423;Name=rev primer;Parent=BBa_K629003
BBa_K1412002	.	stop_codon	2118	2120	.	+	0	ID=annotation2154424;Name=terminator;Parent=BBa_K629003
BBa_K1412002	.	CDS	1476	2120	.	+	0	ID=annotation2154425;Name=CheZ;Parent=BBa_K629003
BBa_K1412002	.	sequence_alteration	2118	2120	.	+	0	ID=annotation2155668;Name=TGA to TAA;Parent=BBa_K629003
BBa_K1412002	.	engineered_region	1	1241	.	+	0	ID=BBa_I732820;Name=BBa_I732820
BBa_K1412002	.	CDS	19	1104	.	+	0	ID=BBa_I732100;Name=BBa_I732100;Parent=BBa_I732820
BBa_K1412002	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732820
BBa_K1412002	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1412002	.	terminator	1113	1192	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I732820
BBa_K1412002	.	stem_loop	1124	1167	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1412002	.	engineered_region	1113	1192	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1412002	.	terminator	1201	1241	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I732820
BBa_K1412002	.	polyA_site	1228	1241	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1412002	.	stop_codon	1234	1234	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1412002	.	stem_loop	1208	1227	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1412002	.	engineered_region	1201	1241	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1412002	.	ribosome_entry_site	1458	1469	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1412002	.	conserved_region	1462	1465	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1412002	.	terminator	2129	2257	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1412002	.	terminator	2217	2257	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1412002	.	polyA_site	2244	2257	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1412002	.	stop_codon	2250	2250	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1412002	.	stem_loop	2224	2243	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1412002	.	engineered_region	2217	2257	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1412002	.	terminator	2129	2208	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1412002	.	stem_loop	2140	2183	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1412002	.	engineered_region	2129	2208	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1412002	.	promoter	1250	1449	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K1412002	.	non_covalent_binding_site	1415	1449	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1412002	.	non_covalent_binding_site	1338	1375	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1412002	.	promoter	1386	1391	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1412002	.	start_codon	1422	1422	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1412002	.	engineered_region	1250	1449	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1412002	.	CDS	1250	1337	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1412002	.	promoter	1410	1415	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
>BBa_K1412002
aaagaggagaaatactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgttt
cccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaatta
cattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctg
cacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtag
aacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaacta
tccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgac
cagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtc
accagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatct
cactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaa
atgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccatta
ccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatccc
gccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggc
caggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccg
cctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataata
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgc
ctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaa
cgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtgga
attgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgatgcaaccatcaatcaaacctgc
tgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactg
gggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatga
ccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaa
agcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagataca
cgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggatt
ttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgct
gttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgatacc
agcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagtcttggattttaatactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttata