LOCUS       BBa_K1412006             799 bp    DNA     linear       27-Jan-2026
DEFINITION  RBS (0.01) CheZ double terminators -- derivative of BBa_K1412005
ACCESSION   BBa_K1412006
VERSION     BBa_K1412006.1  
FEATURES             Location/Qualifiers
     RBS             1..11
                     /label=RBS-4\Weaker
     RBS             7..10
                     /label=RBS
     misc_feature    1..11
                     /label=BBa_B0033
     RBS             1..11
                     /label=BBa_B0033
     primer_bind     18..37
                     /label=fwd primer
     misc_feature    18..20
                     /label=start
     primer_bind     641..662
                     /label=rev primer
     misc_feature    660..662
                     /label=terminator
     CDS             18..662
                     /label=CheZ
     misc_feature    660..662
                     /label=TGA to TAA
     CDS             18..662
                     /label=BBa_K629003
     stem_loop       682..725
                     /label=stem_loop
     misc_feature    671..750
                     /label=BBa_B0010
     terminator      671..750
                     /label=BBa_B0010
     stem_loop       766..785
                     /label=T7 TE
     misc_feature    792..792
                     /label=stop
     polyA_site      786..799
                     /label=polya
     misc_feature    759..799
                     /label=BBa_B0012
     terminator      759..799
                     /label=BBa_B0012
     terminator      671..799
                     /label=BBa_B0015
ORIGIN
        1 tcacacagga ctactagatg atgcaaccat caatcaaacc tgctgacgag cattcagctg
       61 gcgatatcat tgcgcgcatc ggcagcctga cgcgtatgct gcgcgacagt ttgcgggaac
      121 tggggctgga tcaggccatt gccgaagcgg cggaagccat ccccgatgcg cgcgatcgtt
      181 tgtactatgt tgtgcagatg accgcccagg ctgcggagcg ggcgctgaac agtgttgagg
      241 cgtcacaacc gcatcaggat caaatggaga aatcagcaaa agcgttaacc caacgttggg
      301 atgactggtt tgccgatccg attgaccttg ccgacgcccg tgaactggta acagatacac
      361 gacaatttct ggcagatgta cccgcgcata ccagctttac taacgcgcaa ctgctggaaa
      421 tcatgatggc gcaggatttt caggatctca ccgggcaggt cattaagcgg atgatggatg
      481 tcattcagga gatcgaacgc cagttgctga tggtgctgtt ggaaaacatc ccggaacagg
      541 agtcgcgtcc aaaacgtgaa aaccagagtt tgcttaatgg acctcaggtc gataccagca
      601 aagccggtgt ggtagccagt caggatcagg tggacgattt gttggatagt cttggatttt
      661 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      721 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      781 gggcctttct gcgtttata
//