##gff-version 3
##sequence-region BBa_K1412008 1 886
BBa_K1412008	.	promoter	1	78	.	+	0	ID=BBa_R0083;Name=BBa_R0083
BBa_K1412008	.	promoter	68	73	.	+	0	ID=annotation301226;Name=-10;Parent=BBa_R0083
BBa_K1412008	.	point_mutation	41	41	.	+	0	ID=annotation306881;Name=A;Parent=BBa_R0083
BBa_K1412008	.	promoter	45	50	.	+	0	ID=annotation301224;Name=-35;Parent=BBa_R0083
BBa_K1412008	.	sequence_feature	37	44	.	+	0	ID=annotation301223;Name=linker;Parent=BBa_R0083
BBa_K1412008	.	non_covalent_binding_site	13	30	.	+	0	ID=annotation301221;Name=C1 OmpR;Parent=BBa_R0083
BBa_K1412008	.	terminator	758	886	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1412008	.	terminator	758	837	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1412008	.	stem_loop	769	812	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1412008	.	engineered_region	758	837	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1412008	.	terminator	846	886	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1412008	.	engineered_region	846	886	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1412008	.	stop_codon	879	879	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1412008	.	stem_loop	853	872	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1412008	.	polyA_site	873	886	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1412008	.	ribosome_entry_site	87	98	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1412008	.	conserved_region	91	94	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1412008	.	CDS	105	749	.	+	0	ID=BBa_K629003;Name=BBa_K629003
BBa_K1412008	.	start_codon	105	107	.	+	0	ID=annotation2154422;Name=start;Parent=BBa_K629003
BBa_K1412008	.	primer_binding_site	105	124	.	+	0	ID=annotation2154421;Name=fwd primer;Parent=BBa_K629003
BBa_K1412008	.	sequence_alteration	747	749	.	+	0	ID=annotation2155668;Name=TGA to TAA;Parent=BBa_K629003
BBa_K1412008	.	CDS	105	749	.	+	0	ID=annotation2154425;Name=CheZ;Parent=BBa_K629003
BBa_K1412008	.	primer_binding_site	728	749	.	+	0	ID=annotation2154423;Name=rev primer;Parent=BBa_K629003
BBa_K1412008	.	stop_codon	747	749	.	+	0	ID=annotation2154424;Name=terminator;Parent=BBa_K629003
>BBa_K1412008
tcccttgcatttacattttgaaacatctatagcgatctcttgagttggattattctgcatttttggggagaatggactt
actagagaaagaggagaaatactagatgatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcatt
gcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcgg
cggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaa
cagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactgg
tttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgc
ataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaa
gcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcg
cgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcagg
atcaggtggacgatttgttggatagtcttggattttaatactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttata