##gff-version 3
##sequence-region BBa_K1412009 1 2319
BBa_K1412009	.	ribosome_entry_site	1520	1531	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1412009	.	conserved_region	1524	1527	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1412009	.	promoter	1312	1511	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K1412009	.	non_covalent_binding_site	1400	1437	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1412009	.	non_covalent_binding_site	1477	1511	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1412009	.	promoter	1448	1453	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1412009	.	engineered_region	1312	1511	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1412009	.	promoter	1472	1477	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1412009	.	start_codon	1484	1484	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1412009	.	CDS	1312	1399	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1412009	.	CDS	1538	2182	.	+	0	ID=BBa_K629003;Name=BBa_K629003
BBa_K1412009	.	primer_binding_site	1538	1557	.	+	0	ID=annotation2154421;Name=fwd primer;Parent=BBa_K629003
BBa_K1412009	.	start_codon	1538	1540	.	+	0	ID=annotation2154422;Name=start;Parent=BBa_K629003
BBa_K1412009	.	primer_binding_site	2161	2182	.	+	0	ID=annotation2154423;Name=rev primer;Parent=BBa_K629003
BBa_K1412009	.	stop_codon	2180	2182	.	+	0	ID=annotation2154424;Name=terminator;Parent=BBa_K629003
BBa_K1412009	.	sequence_alteration	2180	2182	.	+	0	ID=annotation2155668;Name=TGA to TAA;Parent=BBa_K629003
BBa_K1412009	.	CDS	1538	2182	.	+	0	ID=annotation2154425;Name=CheZ;Parent=BBa_K629003
BBa_K1412009	.	terminator	2191	2319	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1412009	.	terminator	2191	2270	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1412009	.	engineered_region	2191	2270	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1412009	.	stem_loop	2202	2245	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1412009	.	terminator	2279	2319	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1412009	.	stop_codon	2312	2312	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1412009	.	polyA_site	2306	2319	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1412009	.	stem_loop	2286	2305	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1412009	.	engineered_region	2279	2319	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1412009	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1412009	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1412009	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1412009	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1412009	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1412009	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1412009	.	engineered_region	63	1303	.	+	0	ID=BBa_I732820;Name=BBa_I732820
BBa_K1412009	.	terminator	1263	1303	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I732820
BBa_K1412009	.	stop_codon	1296	1296	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1412009	.	polyA_site	1290	1303	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1412009	.	stem_loop	1270	1289	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1412009	.	engineered_region	1263	1303	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1412009	.	terminator	1175	1254	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I732820
BBa_K1412009	.	engineered_region	1175	1254	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1412009	.	stem_loop	1186	1229	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1412009	.	CDS	81	1166	.	+	0	ID=BBa_I732100;Name=BBa_I732100;Parent=BBa_I732820
BBa_K1412009	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732820
BBa_K1412009	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K1412009
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccag
gccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtgg
cacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaat
tgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaa
gcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccagg
atgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacag
tattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctg
ttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattc
agccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcat
cgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgc
gttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatca
aacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaa
tcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttg
gccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataatactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttgg
ccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagtt
agctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataaca
atttcacacatactagagaaagaggagaaatactagatgatgcaaccatcaatcaaacctgctgacgagcattcagctg
gcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccat
tgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggag
cgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgtt
gggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcaga
tgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccggg
caggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccgg
aacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggt
agccagtcaggatcaggtggacgatttgttggatagtcttggattttaatactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttata