BBa_K1412009
type: ComponentDefinition
persistent identity: BBa K1412009
display id: BBa_K1412009
version: 1
was generated by: iGEM to SBOL conversion
description: asddff
created: 2014-09-04T11:00:00Z
modified: 2015-05-08T01:10:18Z
mutable provenance: zsffd
owned by: james
owned by: myers
mutable description: asfghj
discontinued: false
dominant: false
group u list: _1790_
m user id: 0
owner id: 22495
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: zxcxvb
star: false
creator: Tangduo Zhang
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_R0040
component: BBa_R0010
component: BBa_I732820
component: BBa_K629003
component: BBa_B0034
component: BBa_B0015
sequence annotation: BBa_R0040
sequence annotation: BBa_I732820
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K629003
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1412009_sequence/1

BBa_R0040
type: Component
persistent identity: component2391718
display id: component2391718
version: 1
top level: BBa_K1412009
definition: p(tetR)
access: public

BBa_R0010
type: Component
persistent identity: component2391733
display id: component2391733
version: 1
top level: BBa_K1412009
definition: LacI
access: public

BBa_I732820
type: Component
persistent identity: component2391732
display id: component2391732
version: 1
top level: BBa_K1412009
definition: BBa_I732820
access: public

BBa_K629003
type: Component
persistent identity: component2391748
display id: component2391748
version: 1
top level: BBa_K1412009
definition: cheZ
access: public

BBa_B0034
type: Component
persistent identity: component2391741
display id: component2391741
version: 1
top level: BBa_K1412009
definition: BBa_B0034
access: public

BBa_B0015
type: Component
persistent identity: component2391755
display id: component2391755
version: 1
top level: BBa_K1412009
definition: BBa_B0015
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2391718
display id: annotation2391718
version: 1
top level: BBa_K1412009
location: https://synbiohub.org/public/igem/BBa_K1412009/annotation2391718/range2391718/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1412009/annotation2391718/range2391718/1
type: Range
persistent identity: range2391718
display id: range2391718
version: 1
top level: BBa_K1412009
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_I732820
type: SequenceAnnotation
persistent identity: annotation2391732
display id: annotation2391732
version: 1
top level: BBa_K1412009
location: https://synbiohub.org/public/igem/BBa_K1412009/annotation2391732/range2391732/1
component: BBa_I732820

https://synbiohub.org/public/igem/BBa_K1412009/annotation2391732/range2391732/1
type: Range
persistent identity: range2391732
display id: range2391732
version: 1
top level: BBa_K1412009
direction: direction/reverse
start: 63
end: 1303
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2391733
display id: annotation2391733
version: 1
top level: BBa_K1412009
location: https://synbiohub.org/public/igem/BBa_K1412009/annotation2391733/range2391733/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K1412009/annotation2391733/range2391733/1
type: Range
persistent identity: range2391733
display id: range2391733
version: 1
top level: BBa_K1412009
direction: direction/forward
start: 1312
end: 1511
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2391741
display id: annotation2391741
version: 1
top level: BBa_K1412009
location: https://synbiohub.org/public/igem/BBa_K1412009/annotation2391741/range2391741/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1412009/annotation2391741/range2391741/1
type: Range
persistent identity: range2391741
display id: range2391741
version: 1
top level: BBa_K1412009
direction: direction/reverse
start: 1520
end: 1531
orientation: inline

BBa_K629003
type: SequenceAnnotation
persistent identity: annotation2391748
display id: annotation2391748
version: 1
top level: BBa_K1412009
location: https://synbiohub.org/public/igem/BBa_K1412009/annotation2391748/range2391748/1
component: BBa_K629003

https://synbiohub.org/public/igem/BBa_K1412009/annotation2391748/range2391748/1
type: Range
persistent identity: range2391748
display id: range2391748
version: 1
top level: BBa_K1412009
direction: direction/reverse
start: 1538
end: 2182
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2391755
display id: annotation2391755
version: 1
top level: BBa_K1412009
location: https://synbiohub.org/public/igem/BBa_K1412009/annotation2391755/range2391755/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1412009/annotation2391755/range2391755/1
type: Range
persistent identity: range2391755
display id: range2391755
version: 1
top level: BBa_K1412009
direction: direction/reverse
start: 2191
end: 2319
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

cheZ
type: ComponentDefinition
persistent identity: BBa K629003
display id: BBa_K629003
version: 1
was generated by: iGEM to SBOL conversion
description: cheZ, chemotaxis regulator, protein phosphatase for CheY
created: 2011-10-02T11:00:00Z
modified: 2015-05-08T01:12:56Z
mutable provenance: genome of E. Coli BL21 plys strain
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Polar localization is dependent on CheA-short. CheZ is a cytosolic phosphatase which functions in the chemotaxis signal transduction complex. Plays an important role in bacterial chemotaxis signal transduction pathway by accelerating the dephosphorylation of phosphorylated CheY (CheY-P).
discontinued: false
dominant: false
experience: experience/Works
group u list: _801_
m user id: 0
owner id: 9022
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: how to control the proper expression of cheZ
star: false
creator: Zilong WANG, Yi ZHENG
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: CheZ
sequence annotation: fwd primer
sequence annotation: start
sequence annotation: rev primer
sequence annotation: TGA to TAA
sequence annotation: terminator
sequence: https://synbiohub.org/public/igem/BBa_K629003_sequence/1

CheZ
type: SequenceAnnotation
persistent identity: annotation2154425
display id: annotation2154425
version: 1
top level: cheZ
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2154425/range2154425/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K629003/annotation2154425/range2154425/1
type: Range
persistent identity: range2154425
display id: range2154425
version: 1
top level: cheZ
direction: direction/forward
start: 1
end: 645
orientation: inline

fwd primer
type: SequenceAnnotation
persistent identity: annotation2154421
display id: annotation2154421
version: 1
top level: cheZ
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2154421/range2154421/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_K629003/annotation2154421/range2154421/1
type: Range
persistent identity: range2154421
display id: range2154421
version: 1
top level: cheZ
direction: direction/forward
start: 1
end: 20
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2154422
display id: annotation2154422
version: 1
top level: cheZ
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2154422/range2154422/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K629003/annotation2154422/range2154422/1
type: Range
persistent identity: range2154422
display id: range2154422
version: 1
top level: cheZ
direction: direction/forward
start: 1
end: 3
orientation: inline

rev primer
type: SequenceAnnotation
persistent identity: annotation2154423
display id: annotation2154423
version: 1
top level: cheZ
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2154423/range2154423/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_K629003/annotation2154423/range2154423/1
type: Range
persistent identity: range2154423
display id: range2154423
version: 1
top level: cheZ
direction: direction/forward
start: 624
end: 645
orientation: inline

TGA to TAA
type: SequenceAnnotation
persistent identity: annotation2155668
display id: annotation2155668
version: 1
top level: cheZ
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2155668/range2155668/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K629003/annotation2155668/range2155668/1
type: Range
persistent identity: range2155668
display id: range2155668
version: 1
top level: cheZ
direction: direction/forward
start: 643
end: 645
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2154424
display id: annotation2154424
version: 1
top level: cheZ
location: https://synbiohub.org/public/igem/BBa_K629003/annotation2154424/range2154424/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K629003/annotation2154424/range2154424/1
type: Range
persistent identity: range2154424
display id: range2154424
version: 1
top level: cheZ
direction: direction/forward
start: 643
end: 645
orientation: inline

BBa_I732820
type: ComponentDefinition
persistent identity: BBa I732820
display id: BBa_I732820
version: 1
was generated by: iGEM to SBOL conversion
description: LacI (RBS+, TERM+)
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:59Z
mutable provenance: N/A
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: N/A
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: Zhan Jian
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_B0010
component: BBa_I732100
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_I732100
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I732820_sequence/1

BBa_B0034
type: Component
persistent identity: component1946760
display id: component1946760
version: 1
top level: BBa_I732820
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component1946762
display id: component1946762
version: 1
top level: BBa_I732820
definition: BBa_B0010
access: public

BBa_I732100
type: Component
persistent identity: component1946761
display id: component1946761
version: 1
top level: BBa_I732820
definition: LacI
access: public

BBa_B0012
type: Component
persistent identity: component1946764
display id: component1946764
version: 1
top level: BBa_I732820
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1946760
display id: annotation1946760
version: 1
top level: BBa_I732820
location: https://synbiohub.org/public/igem/BBa_I732820/annotation1946760/range1946760/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732820/annotation1946760/range1946760/1
type: Range
persistent identity: range1946760
display id: range1946760
version: 1
top level: BBa_I732820
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_I732100
type: SequenceAnnotation
persistent identity: annotation1946761
display id: annotation1946761
version: 1
top level: BBa_I732820
location: https://synbiohub.org/public/igem/BBa_I732820/annotation1946761/range1946761/1
component: BBa_I732100

https://synbiohub.org/public/igem/BBa_I732820/annotation1946761/range1946761/1
type: Range
persistent identity: range1946761
display id: range1946761
version: 1
top level: BBa_I732820
direction: direction/reverse
start: 19
end: 1104
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1946762
display id: annotation1946762
version: 1
top level: BBa_I732820
location: https://synbiohub.org/public/igem/BBa_I732820/annotation1946762/range1946762/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732820/annotation1946762/range1946762/1
type: Range
persistent identity: range1946762
display id: range1946762
version: 1
top level: BBa_I732820
direction: direction/forward
start: 1113
end: 1192
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1946764
display id: annotation1946764
version: 1
top level: BBa_I732820
location: https://synbiohub.org/public/igem/BBa_I732820/annotation1946764/range1946764/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732820/annotation1946764/range1946764/1
type: Range
persistent identity: range1946764
display id: range1946764
version: 1
top level: BBa_I732820
direction: direction/forward
start: 1201
end: 1241
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa I732100
display id: BBa_I732100
version: 1
was generated by: iGEM to SBOL conversion
description: LacI
created: 2007-10-13T11:00:00Z
modified: 2016-10-18T06:58:21Z
mutable provenance: N/A
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: N/A
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 31248
owner id: 1557
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: Zhan Jian
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_I732100_sequence/1

https://synbiohub.org/public/igem/BBa_K1412009_sequence/1
type: Sequence
persistent identity: BBa K1412009 sequence
display id: BBa_K1412009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K629003_sequence/1
type: Sequence
persistent identity: BBa K629003 sequence
display id: BBa_K629003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagtcttggattttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732820_sequence/1
type: Sequence
persistent identity: BBa I732820 sequence
display id: BBa_I732820_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732100_sequence/1
type: Sequence
persistent identity: BBa I732100 sequence
display id: BBa_I732100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z