LOCUS       BBa_K1412010             916 bp    DNA     linear       19-Jan-2026
DEFINITION  Drive the E.coli engineered strain(CL-1) towards death region
ACCESSION   BBa_K1412010
VERSION     BBa_K1412010.1  
FEATURES             Location/Qualifiers
     misc_feature    13..30
                     /label=C1 OmpR
     misc_feature    34..51
                     /label=C2 OmpR
     misc_feature    54..71
                     /label=C3 OmpR
     promoter        75..80
                     /label=-35
     promoter        98..103
                     /label=-10
     promoter        1..108
                     /label=BBa_R0082
     misc_feature    121..124
                     /label=conserved
     RBS             117..128
                     /label=BBa_B0034
     primer_bind     135..154
                     /label=fwd primer
     misc_feature    135..137
                     /label=start
     primer_bind     758..779
                     /label=rev primer
     misc_feature    777..779
                     /label=terminator
     CDS             135..779
                     /label=CheZ
     misc_feature    777..779
                     /label=TGA to TAA
     CDS             135..779
                     /label=BBa_K629003
     stem_loop       799..842
                     /label=stem_loop
     misc_feature    788..867
                     /label=BBa_B0010
     terminator      788..867
                     /label=BBa_B0010
     stem_loop       883..902
                     /label=T7 TE
     misc_feature    909..909
                     /label=stop
     polyA_site      903..916
                     /label=polya
     misc_feature    876..916
                     /label=BBa_B0012
     terminator      876..916
                     /label=BBa_B0012
     terminator      788..916
                     /label=BBa_B0015
ORIGIN
        1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta
       61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagaaag
      121 aggagaaata ctagatgatg caaccatcaa tcaaacctgc tgacgagcat tcagctggcg
      181 atatcattgc gcgcatcggc agcctgacgc gtatgctgcg cgacagtttg cgggaactgg
      241 ggctggatca ggccattgcc gaagcggcgg aagccatccc cgatgcgcgc gatcgtttgt
      301 actatgttgt gcagatgacc gcccaggctg cggagcgggc gctgaacagt gttgaggcgt
      361 cacaaccgca tcaggatcaa atggagaaat cagcaaaagc gttaacccaa cgttgggatg
      421 actggtttgc cgatccgatt gaccttgccg acgcccgtga actggtaaca gatacacgac
      481 aatttctggc agatgtaccc gcgcatacca gctttactaa cgcgcaactg ctggaaatca
      541 tgatggcgca ggattttcag gatctcaccg ggcaggtcat taagcggatg atggatgtca
      601 ttcaggagat cgaacgccag ttgctgatgg tgctgttgga aaacatcccg gaacaggagt
      661 cgcgtccaaa acgtgaaaac cagagtttgc ttaatggacc tcaggtcgat accagcaaag
      721 ccggtgtggt agccagtcag gatcaggtgg acgatttgtt ggatagtctt ggattttaat
      781 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
      841 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
      901 cctttctgcg tttata
//