##gff-version 3
##sequence-region BBa_K1412010 1 916
BBa_K1412010	.	ribosome_entry_site	117	128	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1412010	.	conserved_region	121	124	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1412010	.	terminator	788	916	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1412010	.	terminator	876	916	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1412010	.	stem_loop	883	902	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1412010	.	engineered_region	876	916	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1412010	.	stop_codon	909	909	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1412010	.	polyA_site	903	916	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1412010	.	terminator	788	867	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1412010	.	engineered_region	788	867	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1412010	.	stem_loop	799	842	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1412010	.	CDS	135	779	.	+	0	ID=BBa_K629003;Name=BBa_K629003
BBa_K1412010	.	primer_binding_site	758	779	.	+	0	ID=annotation2154423;Name=rev primer;Parent=BBa_K629003
BBa_K1412010	.	primer_binding_site	135	154	.	+	0	ID=annotation2154421;Name=fwd primer;Parent=BBa_K629003
BBa_K1412010	.	sequence_alteration	777	779	.	+	0	ID=annotation2155668;Name=TGA to TAA;Parent=BBa_K629003
BBa_K1412010	.	stop_codon	777	779	.	+	0	ID=annotation2154424;Name=terminator;Parent=BBa_K629003
BBa_K1412010	.	start_codon	135	137	.	+	0	ID=annotation2154422;Name=start;Parent=BBa_K629003
BBa_K1412010	.	CDS	135	779	.	+	0	ID=annotation2154425;Name=CheZ;Parent=BBa_K629003
BBa_K1412010	.	promoter	1	108	.	+	0	ID=BBa_R0082;Name=BBa_R0082
BBa_K1412010	.	promoter	75	80	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_K1412010	.	non_covalent_binding_site	54	71	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_K1412010	.	promoter	98	103	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_K1412010	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_K1412010	.	non_covalent_binding_site	13	30	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
>BBa_K1412010
tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttgga
ttattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatgatgcaaccatcaatcaaacct
gctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaac
tggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagat
gaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagca
aaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagata
cacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcagga
ttttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtg
ctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgata
ccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagtcttggattttaatactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttata