LOCUS       BBa_K1412014             862 bp    DNA     linear       25-Nov-2024
DEFINITION  Characterize the efficiency of pTetR (R0040) with chemotaxis
ACCESSION   BBa_K1412014
VERSION     BBa_K1412014.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     primer_bind     81..100
                     /label=fwd primer
     misc_feature    81..83
                     /label=start
     primer_bind     704..725
                     /label=rev primer
     misc_feature    723..725
                     /label=terminator
     CDS             81..725
                     /label=CheZ
     misc_feature    723..725
                     /label=TGA to TAA
     CDS             81..725
                     /label=BBa_K629003
     stem_loop       745..788
                     /label=stem_loop
     misc_feature    734..813
                     /label=BBa_B0010
     terminator      734..813
                     /label=BBa_B0010
     stem_loop       829..848
                     /label=T7 TE
     misc_feature    855..855
                     /label=stop
     polyA_site      849..862
                     /label=polya
     misc_feature    822..862
                     /label=BBa_B0012
     terminator      822..862
                     /label=BBa_B0012
     terminator      734..862
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgatgcaac catcaatcaa acctgctgac gagcattcag
      121 ctggcgatat cattgcgcgc atcggcagcc tgacgcgtat gctgcgcgac agtttgcggg
      181 aactggggct ggatcaggcc attgccgaag cggcggaagc catccccgat gcgcgcgatc
      241 gtttgtacta tgttgtgcag atgaccgccc aggctgcgga gcgggcgctg aacagtgttg
      301 aggcgtcaca accgcatcag gatcaaatgg agaaatcagc aaaagcgtta acccaacgtt
      361 gggatgactg gtttgccgat ccgattgacc ttgccgacgc ccgtgaactg gtaacagata
      421 cacgacaatt tctggcagat gtacccgcgc ataccagctt tactaacgcg caactgctgg
      481 aaatcatgat ggcgcaggat tttcaggatc tcaccgggca ggtcattaag cggatgatgg
      541 atgtcattca ggagatcgaa cgccagttgc tgatggtgct gttggaaaac atcccggaac
      601 aggagtcgcg tccaaaacgt gaaaaccaga gtttgcttaa tggacctcag gtcgatacca
      661 gcaaagccgg tgtggtagcc agtcaggatc aggtggacga tttgttggat agtcttggat
      721 tttaatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      781 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
      841 ggtgggcctt tctgcgttta ta
//