##gff-version 3
##sequence-region BBa_K1412014 1 862
BBa_K1412014	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1412014	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1412014	.	CDS	81	725	.	+	0	ID=BBa_K629003;Name=BBa_K629003
BBa_K1412014	.	CDS	81	725	.	+	0	ID=annotation2154425;Name=CheZ;Parent=BBa_K629003
BBa_K1412014	.	primer_binding_site	704	725	.	+	0	ID=annotation2154423;Name=rev primer;Parent=BBa_K629003
BBa_K1412014	.	start_codon	81	83	.	+	0	ID=annotation2154422;Name=start;Parent=BBa_K629003
BBa_K1412014	.	primer_binding_site	81	100	.	+	0	ID=annotation2154421;Name=fwd primer;Parent=BBa_K629003
BBa_K1412014	.	sequence_alteration	723	725	.	+	0	ID=annotation2155668;Name=TGA to TAA;Parent=BBa_K629003
BBa_K1412014	.	stop_codon	723	725	.	+	0	ID=annotation2154424;Name=terminator;Parent=BBa_K629003
BBa_K1412014	.	terminator	734	862	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1412014	.	terminator	822	862	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1412014	.	stem_loop	829	848	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1412014	.	engineered_region	822	862	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1412014	.	stop_codon	855	855	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1412014	.	polyA_site	849	862	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1412014	.	terminator	734	813	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1412014	.	engineered_region	734	813	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1412014	.	stem_loop	745	788	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1412014	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1412014	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1412014	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1412014	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1412014	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1412014	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
>BBa_K1412014
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgt
atgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcg
atcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgca
tcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgcc
gacgcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaac
tgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcagga
gatcgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagt
ttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggata
gtcttggattttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata