##gff-version 3
##sequence-region BBa_K1412033 1 3210
BBa_K1412033	.	promoter	892	945	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1412033	.	promoter	934	939	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1412033	.	non_covalent_binding_site	892	910	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1412033	.	engineered_region	892	945	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1412033	.	non_covalent_binding_site	917	935	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1412033	.	promoter	911	916	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1412033	.	promoter	2203	2402	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K1412033	.	non_covalent_binding_site	2291	2328	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1412033	.	promoter	2339	2344	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1412033	.	promoter	2363	2368	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1412033	.	start_codon	2375	2375	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1412033	.	engineered_region	2203	2402	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1412033	.	CDS	2203	2290	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1412033	.	non_covalent_binding_site	2368	2402	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1412033	.	engineered_region	1	883	.	+	0	ID=BBa_K145201;Name=BBa_K145201
BBa_K1412033	.	CDS	62	746	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K145201
BBa_K1412033	.	tag	682	715	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1412033	.	CDS	65	681	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1412033	.	engineered_tag	722	746	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1412033	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K145201
BBa_K1412033	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1412033	.	promoter	1	35	.	+	0	ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_K145201
BBa_K1412033	.	terminator	755	883	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K145201
BBa_K1412033	.	terminator	843	883	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1412033	.	polyA_site	870	883	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1412033	.	stem_loop	850	869	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1412033	.	stop_codon	876	876	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1412033	.	engineered_region	843	883	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1412033	.	terminator	755	834	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1412033	.	stem_loop	766	809	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1412033	.	engineered_region	755	834	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1412033	.	engineered_region	954	2194	.	+	0	ID=BBa_I732820;Name=BBa_I732820
BBa_K1412033	.	terminator	2154	2194	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I732820
BBa_K1412033	.	polyA_site	2181	2194	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1412033	.	stem_loop	2161	2180	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1412033	.	stop_codon	2187	2187	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1412033	.	engineered_region	2154	2194	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1412033	.	ribosome_entry_site	954	965	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732820
BBa_K1412033	.	conserved_region	958	961	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1412033	.	CDS	972	2057	.	+	0	ID=BBa_I732100;Name=BBa_I732100;Parent=BBa_I732820
BBa_K1412033	.	terminator	2066	2145	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I732820
BBa_K1412033	.	stem_loop	2077	2120	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1412033	.	engineered_region	2066	2145	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1412033	.	terminator	3082	3210	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1412033	.	terminator	3170	3210	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1412033	.	polyA_site	3197	3210	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1412033	.	stem_loop	3177	3196	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1412033	.	stop_codon	3203	3203	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1412033	.	engineered_region	3170	3210	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1412033	.	terminator	3082	3161	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1412033	.	stem_loop	3093	3136	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1412033	.	engineered_region	3082	3161	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1412033	.	ribosome_entry_site	2411	2422	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1412033	.	conserved_region	2415	2418	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1412033	.	CDS	2429	3073	.	+	0	ID=BBa_K629003;Name=BBa_K629003
BBa_K1412033	.	CDS	2429	3073	.	+	0	ID=annotation2154425;Name=CheZ;Parent=BBa_K629003
BBa_K1412033	.	primer_binding_site	2429	2448	.	+	0	ID=annotation2154421;Name=fwd primer;Parent=BBa_K629003
BBa_K1412033	.	primer_binding_site	3052	3073	.	+	0	ID=annotation2154423;Name=rev primer;Parent=BBa_K629003
BBa_K1412033	.	start_codon	2429	2431	.	+	0	ID=annotation2154422;Name=start;Parent=BBa_K629003
BBa_K1412033	.	sequence_alteration	3071	3073	.	+	0	ID=annotation2155668;Name=TGA to TAA;Parent=BBa_K629003
BBa_K1412033	.	stop_codon	3071	3073	.	+	0	ID=annotation2154424;Name=terminator;Parent=BBa_K629003
>BBa_K1412033
ttgacagctagctcagtcctagggactatgctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt
agcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactac
tagagaaagaggagaaatactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagac
cgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctg
aattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctgg
ccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgat
ggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcatt
aactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtct
ctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcatt
gggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaa
tatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaacca
tgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgc
cattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttat
atcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctc
agggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgca
aaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaa
taatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcg
gtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaa
accgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgag
cgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgt
gtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgatgcaaccatcaatcaaa
cctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcggg
aactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgca
gatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatca
gcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacag
atacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgca
ggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatg
gtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcg
ataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagtcttggattttaatactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata