LOCUS BBa_K1412600 3938 bp DNA linear 22-Dec-2024 DEFINITION Endow the CL-1(E.coli engineered strain) the ability of chemotaxis and quorum sensing ACCESSION BBa_K1412600 VERSION BBa_K1412600.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 164..167 /label=conserved RBS 160..171 /label=BBa_B0034 misc_feature 178..179 /label=prefix misc_feature 351..351 /label=T misc_feature 669..669 /label=A CDS 178..927 /label=luxR misc_feature 178..933 /label=BBa_C0062 misc_feature 934..958 /label=Help:Barcodes CDS 178..933 /label=BBa_C0062 stem_loop 978..1021 /label=stem_loop misc_feature 967..1046 /label=BBa_B0010 terminator 967..1046 /label=BBa_B0010 stem_loop 1062..1081 /label=T7 TE misc_feature 1088..1088 /label=stop polyA_site 1082..1095 /label=polya misc_feature 1055..1095 /label=BBa_B0012 terminator 1055..1095 /label=BBa_B0012 misc_feature 1104..1123 /label=LuxR/HSL promoter 1123..1128 /label=-35 promoter 1145..1150 /label=-10 misc_feature 1156..1156 /label=start misc_feature 1104..1158 /label=BBa_R0062 promoter 1104..1158 /label=BBa_R0062 misc_feature 1..1158 /label=BBa_F2621 misc_feature 1171..1174 /label=conserved RBS 1167..1178 /label=BBa_B0034 misc_feature 1764..1795 /label=LVA CDS 1185..1763 /label=luxI misc_feature 1185..1802 /label=BBa_C0061 misc_feature 1803..1827 /label=Help:Barcodes CDS 1185..1802 /label=BBa_C0061 misc_feature 1167..1827 /label=BBa_C0261 stem_loop 1847..1890 /label=stem_loop misc_feature 1836..1915 /label=BBa_B0010 terminator 1836..1915 /label=BBa_B0010 stem_loop 1931..1950 /label=T7 TE misc_feature 1957..1957 /label=stop polyA_site 1951..1964 /label=polya misc_feature 1924..1964 /label=BBa_B0012 terminator 1924..1964 /label=BBa_B0012 terminator 1836..1964 /label=BBa_B0015 misc_feature 1167..1964 /label=BBa_K082014 misc_feature 1..1964 /label=BBa_K546000 misc_feature 1973..1992 /label=LuxR/HSL promoter 2087..2094 /label=-10 promoter 2061..2066 /label=-35 misc_feature 2100..2100 /label=start misc_feature 2102..2121 /label=Putative LuxR/HSL misc_feature 1973..2123 /label=BBa_R0063 promoter 1973..2123 /label=BBa_R0063 misc_feature 2136..2139 /label=conserved RBS 2132..2143 /label=BBa_B0034 misc_feature 2150..2151 /label=prefix misc_feature 2323..2323 /label=T misc_feature 2641..2641 /label=A CDS 2150..2899 /label=luxR misc_feature 2150..2905 /label=BBa_C0062 misc_feature 2906..2930 /label=Help:Barcodes CDS 2150..2905 /label=BBa_C0062 stem_loop 2950..2993 /label=stem_loop misc_feature 2939..3018 /label=BBa_B0010 terminator 2939..3018 /label=BBa_B0010 stem_loop 3034..3053 /label=T7 TE misc_feature 3060..3060 /label=stop polyA_site 3054..3067 /label=polya misc_feature 3027..3067 /label=BBa_B0012 terminator 3027..3067 /label=BBa_B0012 misc_feature 3076..3095 /label=LuxR/HSL promoter 3095..3100 /label=-35 promoter 3117..3122 /label=-10 misc_feature 3128..3128 /label=start misc_feature 3076..3130 /label=BBa_R0062 promoter 3076..3130 /label=BBa_R0062 misc_feature 1973..3130 /label=BBa_F2621 misc_feature 3143..3146 /label=conserved RBS 3139..3150 /label=BBa_B0034 primer_bind 3157..3176 /label=fwd primer misc_feature 3157..3159 /label=start primer_bind 3780..3801 /label=rev primer misc_feature 3799..3801 /label=terminator CDS 3157..3801 /label=CheZ misc_feature 3799..3801 /label=TGA to TAA CDS 3157..3801 /label=BBa_K629003 stem_loop 3821..3864 /label=stem_loop misc_feature 3810..3889 /label=BBa_B0010 terminator 3810..3889 /label=BBa_B0010 stem_loop 3905..3924 /label=T7 TE misc_feature 3931..3931 /label=stop polyA_site 3925..3938 /label=polya misc_feature 3898..3938 /label=BBa_B0012 terminator 3898..3938 /label=BBa_B0012 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg 181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt 1141 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatgact ataatgataa 1201 aaaaatcgga ttttttggca attccatcgg aggagtataa aggtattcta agtcttcgtt 1261 atcaagtgtt taagcaaaga cttgagtggg acttagttgt agaaaataac cttgaatcag 1321 atgagtatga taactcaaat gcagaatata tttatgcttg tgatgatact gaaaatgtaa 1381 gtggatgctg gcgtttatta cctacaacag gtgattatat gctgaaaagt gtttttcctg 1441 aattgcttgg tcaacagagt gctcccaaag atcctaatat agtcgaatta agtcgttttg 1501 ctgtaggtaa aaatagctca aagataaata actctgctag tgaaattaca atgaaactat 1561 ttgaagctat atataaacac gctgttagtc aaggtattac agaatatgta acagtaacat 1621 caacagcaat agagcgattt ttaaagcgta ttaaagttcc ttgtcatcgt attggagaca 1681 aagaaattca tgtattaggt gatactaaat cggttgtatt gtctatgcct attaatgaac 1741 agtttaaaaa agcagtctta aatgctgcaa acgacgaaaa ctacgcttta gtagcttaat 1801 aactctgata gtgctagtgt agatctctac tagagccagg catcaaataa aacgaaaggc 1861 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 1921 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agacctgtac 1981 gatcctacag gtgcttatgt taagtaattg tattcccagc gatacaatag tgtgacaaaa 2041 atccaattta ttagaatcaa atgtcaatcc attaccgttt taatgatata taacacgcaa 2101 aacttgcgac aaacaatagg taatactaga gaaagaggag aaatactaga tgaaaaacat 2161 aaatgccgac gacacataca gaataattaa taaaattaaa gcttgtagaa gcaataatga 2221 tattaatcaa tgcttatctg atatgactaa aatggtacat tgtgaatatt atttactcgc 2281 gatcatttat cctcattcta tggttaaatc tgatatttca atcctagata attaccctaa 2341 aaaatggagg caatattatg atgacgctaa tttaataaaa tatgatccta tagtagatta 2401 ttctaactcc aatcattcac caattaattg gaatatattt gaaaacaatg ctgtaaataa 2461 aaaatctcca aatgtaatta aagaagcgaa aacatcaggt cttatcactg ggtttagttt 2521 ccctattcat acggctaaca atggcttcgg aatgcttagt tttgcacatt cagaaaaaga 2581 caactatata gatagtttat ttttacatgc gtgtatgaac ataccattaa ttgttccttc 2641 tctagttgat aattatcgaa aaataaatat agcaaataat aaatcaaaca acgatttaac 2701 caaaagagaa aaagaatgtt tagcgtgggc atgcgaagga aaaagctctt gggatatttc 2761 aaaaatatta ggttgcagtg agcgtactgt cactttccat ttaaccaatg cgcaaatgaa 2821 actcaataca acaaaccgct gccaaagtat ttctaaagca attttaacag gagcaattga 2881 ttgcccatac tttaaaaatt aataacactg atagtgctag tgtagatcac tactagagcc 2941 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 3001 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 3061 gtttatatac tagagacctg taggatcgta caggtttacg caagaaaatg gtttgttata 3121 gtcgaataaa tactagagaa agaggagaaa tactagatga tgcaaccatc aatcaaacct 3181 gctgacgagc attcagctgg cgatatcatt gcgcgcatcg gcagcctgac gcgtatgctg 3241 cgcgacagtt tgcgggaact ggggctggat caggccattg ccgaagcggc ggaagccatc 3301 cccgatgcgc gcgatcgttt gtactatgtt gtgcagatga ccgcccaggc tgcggagcgg 3361 gcgctgaaca gtgttgaggc gtcacaaccg catcaggatc aaatggagaa atcagcaaaa 3421 gcgttaaccc aacgttggga tgactggttt gccgatccga ttgaccttgc cgacgcccgt 3481 gaactggtaa cagatacacg acaatttctg gcagatgtac ccgcgcatac cagctttact 3541 aacgcgcaac tgctggaaat catgatggcg caggattttc aggatctcac cgggcaggtc 3601 attaagcgga tgatggatgt cattcaggag atcgaacgcc agttgctgat ggtgctgttg 3661 gaaaacatcc cggaacagga gtcgcgtcca aaacgtgaaa accagagttt gcttaatgga 3721 cctcaggtcg ataccagcaa agccggtgtg gtagccagtc aggatcaggt ggacgatttg 3781 ttggatagtc ttggatttta atactagagc caggcatcaa ataaaacgaa aggctcagtc 3841 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 3901 cactggctca ccttcgggtg ggcctttctg cgtttata //