##gff-version 3
##sequence-region BBa_K1412600 1 3938
BBa_K1412600	.	terminator	3898	3938	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K1412600	.	polyA_site	3925	3938	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1412600	.	engineered_region	3898	3938	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1412600	.	stem_loop	3905	3924	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1412600	.	stop_codon	3931	3931	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1412600	.	CDS	3157	3801	.	+	0	ID=BBa_K629003;Name=BBa_K629003
BBa_K1412600	.	sequence_alteration	3799	3801	.	+	0	ID=annotation2155668;Name=TGA to TAA;Parent=BBa_K629003
BBa_K1412600	.	CDS	3157	3801	.	+	0	ID=annotation2154425;Name=CheZ;Parent=BBa_K629003
BBa_K1412600	.	primer_binding_site	3780	3801	.	+	0	ID=annotation2154423;Name=rev primer;Parent=BBa_K629003
BBa_K1412600	.	stop_codon	3799	3801	.	+	0	ID=annotation2154424;Name=terminator;Parent=BBa_K629003
BBa_K1412600	.	start_codon	3157	3159	.	+	0	ID=annotation2154422;Name=start;Parent=BBa_K629003
BBa_K1412600	.	primer_binding_site	3157	3176	.	+	0	ID=annotation2154421;Name=fwd primer;Parent=BBa_K629003
BBa_K1412600	.	engineered_region	1973	3130	.	+	0	ID=BBa_F2621;Name=BBa_F2621
BBa_K1412600	.	ribosome_entry_site	2132	2143	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2621
BBa_K1412600	.	conserved_region	2136	2139	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1412600	.	terminator	3027	3067	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F2621
BBa_K1412600	.	polyA_site	3054	3067	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1412600	.	engineered_region	3027	3067	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1412600	.	stem_loop	3034	3053	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1412600	.	stop_codon	3060	3060	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1412600	.	promoter	1973	2123	.	+	0	ID=BBa_R0063;Name=BBa_R0063;Parent=BBa_F2621
BBa_K1412600	.	non_covalent_binding_site	1973	1992	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_K1412600	.	promoter	2061	2066	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_K1412600	.	engineered_region	1973	2123	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_K1412600	.	non_covalent_binding_site	2102	2121	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_K1412600	.	start_codon	2100	2100	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_K1412600	.	promoter	2087	2094	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_K1412600	.	terminator	2939	3018	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F2621
BBa_K1412600	.	engineered_region	2939	3018	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1412600	.	stem_loop	2950	2993	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1412600	.	promoter	3076	3130	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2621
BBa_K1412600	.	non_covalent_binding_site	3076	3095	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1412600	.	promoter	3117	3122	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1412600	.	promoter	3095	3100	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1412600	.	start_codon	3128	3128	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1412600	.	engineered_region	3076	3130	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1412600	.	CDS	2150	2905	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2621
BBa_K1412600	.	engineered_region	2150	2905	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1412600	.	engineered_region	2150	2151	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1412600	.	CDS	2150	2899	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1412600	.	point_mutation	2323	2323	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1412600	.	point_mutation	2641	2641	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1412600	.	engineered_tag	2906	2930	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1412600	.	ribosome_entry_site	3139	3150	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1412600	.	conserved_region	3143	3146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1412600	.	engineered_region	1	1964	.	+	0	ID=BBa_K546000;Name=BBa_K546000
BBa_K1412600	.	engineered_region	1	1158	.	+	0	ID=BBa_F2621;Name=BBa_F2621;Parent=BBa_K546000
BBa_K1412600	.	ribosome_entry_site	160	171	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2621
BBa_K1412600	.	conserved_region	164	167	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1412600	.	terminator	1055	1095	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F2621
BBa_K1412600	.	polyA_site	1082	1095	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1412600	.	engineered_region	1055	1095	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1412600	.	stem_loop	1062	1081	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1412600	.	stop_codon	1088	1088	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1412600	.	promoter	1	151	.	+	0	ID=BBa_R0063;Name=BBa_R0063;Parent=BBa_F2621
BBa_K1412600	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_K1412600	.	promoter	89	94	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_K1412600	.	engineered_region	1	151	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_K1412600	.	non_covalent_binding_site	130	149	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_K1412600	.	start_codon	128	128	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_K1412600	.	promoter	115	122	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_K1412600	.	terminator	967	1046	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F2621
BBa_K1412600	.	engineered_region	967	1046	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1412600	.	stem_loop	978	1021	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1412600	.	promoter	1104	1158	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2621
BBa_K1412600	.	non_covalent_binding_site	1104	1123	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1412600	.	promoter	1145	1150	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1412600	.	promoter	1123	1128	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1412600	.	start_codon	1156	1156	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1412600	.	engineered_region	1104	1158	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1412600	.	CDS	178	933	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2621
BBa_K1412600	.	engineered_region	178	933	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1412600	.	engineered_region	178	179	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1412600	.	CDS	178	927	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1412600	.	point_mutation	351	351	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1412600	.	point_mutation	669	669	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1412600	.	engineered_tag	934	958	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1412600	.	engineered_region	1167	1964	.	+	0	ID=BBa_K082014;Name=BBa_K082014;Parent=BBa_K546000
BBa_K1412600	.	engineered_region	1167	1827	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K082014
BBa_K1412600	.	CDS	1185	1802	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K1412600	.	engineered_region	1185	1802	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K1412600	.	engineered_tag	1803	1827	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K1412600	.	CDS	1185	1763	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K1412600	.	tag	1764	1795	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K1412600	.	ribosome_entry_site	1167	1178	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K1412600	.	conserved_region	1171	1174	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1412600	.	terminator	1836	1964	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K082014
BBa_K1412600	.	terminator	1924	1964	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1412600	.	polyA_site	1951	1964	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1412600	.	engineered_region	1924	1964	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1412600	.	stem_loop	1931	1950	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1412600	.	stop_codon	1957	1957	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1412600	.	terminator	1836	1915	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1412600	.	engineered_region	1836	1915	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1412600	.	stem_loop	1847	1890	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1412600	.	terminator	3810	3889	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K1412600	.	engineered_region	3810	3889	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1412600	.	stem_loop	3821	3864	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K1412600
acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttat
tagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactaga
gaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtaga
agcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattt
atcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgc
taatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaac
aatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttcccta
ttcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttt
acatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaa
tcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaa
aaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctg
ccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctag
tgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacc
tgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactaga
tgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatca
agtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgca
gaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgc
tgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgc
tgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacac
gctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttc
cttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatga
acagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctag
tgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacc
tgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattag
aatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactagagaa
agaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagc
aataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatc
ctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaa
tttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaat
gctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattc
atacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttaca
tgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatca
aacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaa
tattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgcca
aagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgt
agatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgt
aggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatga
tgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgct
gcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgt
ttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcagg
atcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgc
ccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctg
gaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcg
aacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgct
taatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagtctt
ggattttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata