##gff-version 3
##sequence-region BBa_K1412801 1 1007
BBa_K1412801	.	ribosome_entry_site	209	219	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_K1412801	.	ribosome_entry_site	209	219	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K1412801	.	engineered_region	209	219	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K1412801	.	ribosome_entry_site	215	218	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K1412801	.	terminator	879	1007	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1412801	.	terminator	967	1007	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1412801	.	engineered_region	967	1007	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1412801	.	stem_loop	974	993	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1412801	.	polyA_site	994	1007	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1412801	.	stop_codon	1000	1000	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1412801	.	terminator	879	958	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1412801	.	engineered_region	879	958	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1412801	.	stem_loop	890	933	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1412801	.	CDS	226	870	.	+	0	ID=BBa_K629003;Name=BBa_K629003
BBa_K1412801	.	primer_binding_site	226	245	.	+	0	ID=annotation2154421;Name=fwd primer;Parent=BBa_K629003
BBa_K1412801	.	primer_binding_site	849	870	.	+	0	ID=annotation2154423;Name=rev primer;Parent=BBa_K629003
BBa_K1412801	.	sequence_alteration	868	870	.	+	0	ID=annotation2155668;Name=TGA to TAA;Parent=BBa_K629003
BBa_K1412801	.	stop_codon	868	870	.	+	0	ID=annotation2154424;Name=terminator;Parent=BBa_K629003
BBa_K1412801	.	start_codon	226	228	.	+	0	ID=annotation2154422;Name=start;Parent=BBa_K629003
BBa_K1412801	.	CDS	226	870	.	+	0	ID=annotation2154425;Name=CheZ;Parent=BBa_K629003
BBa_K1412801	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K1412801	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1412801	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1412801	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1412801	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1412801	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1412801	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1412801	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
>BBa_K1412801
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggactactagatgatgcaacca
tcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgaca
gtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtacta
tgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatg
gagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaac
tggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcat
gatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccag
ttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggac
ctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagtcttggatttta
atactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata