LOCUS       BBa_K1412924             919 bp    DNA     linear       23-May-2024
DEFINITION  GFP generator with J23101
ACCESSION   BBa_K1412924
VERSION     BBa_K1412924.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=J23101
     misc_feature    1..6
                     /label=-35 box
     misc_feature    24..29
                     /label=-10 box
     promoter        1..35
                     /label=BBa_K823005
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     CDS             63..782
                     /label=GFP protein
     CDS             63..782
                     /label=BBa_E0040
     stem_loop       802..845
                     /label=stem_loop
     misc_feature    791..870
                     /label=BBa_B0010
     terminator      791..870
                     /label=BBa_B0010
     stem_loop       886..905
                     /label=T7 TE
     misc_feature    912..912
                     /label=stop
     polyA_site      906..919
                     /label=polya
     misc_feature    879..919
                     /label=BBa_B0012
     terminator      879..919
                     /label=BBa_B0012
     misc_feature    44..919
                     /label=BBa_E0240
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact
       61 agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
      121 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
      181 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
      241 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac
      301 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
      361 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
      421 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
      481 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
      541 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
      601 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
      661 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
      721 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat
      781 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      841 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      901 gggcctttct gcgtttata
//