LOCUS       BBa_K1413001            2847 bp    DNA     linear       27-Dec-2024
DEFINITION  P0 promoter-RBS B0032-sfGFP- Terminator B0015 - Pr promoter-DmpR
ACCESSION   BBa_K1413001
VERSION     BBa_K1413001.1  
FEATURES             Location/Qualifiers
     promoter        1..171
                     /label=P0 Promoter
     misc_feature    172..179
                     /label=RBS Junction
     RBS             180..192
                     /label=RBS B0032
     misc_feature    193..198
                     /label=RBS Junction
     CDS             199..929
                     /label=sfGFP
     misc_feature    930..1058
                     /label=Terminator B0015
     misc_feature    1059..1066
                     /label=SpeI/XbaI mixed site
     promoter        1082..1155
                     /label=PR Promoter
     CDS             1156..2847
                     /label=DmpR
     misc_feature    1..2847
                     /label=BBa_K1413001
ORIGIN
        1 taagcatttg ctcaagcggc cttgggcaat tgatcaaatg cttaaaaagt ctgcgcaagc
       61 gcggcttaat ttcgctcgct ccgatcattc taaaaattag aaacacattg aaaaacatta
      121 ccttgaagtc tgttttcaga ccttggcaca gccgttgctt gatgtcctgc gtactagagt
      181 cacacaggaa agactagaat gcgtaaaggc gaagagctgt tcactggtgt cgtccctatt
      241 ctggtggaac tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa
      301 ggtgacgcaa ctaatggtaa actgacgctg aagttcatct gtactactgg taaactgccg
      361 gtaccttggc cgactctggt aacgacgctg acttatggtg ttcagtgctt tgctcgttat
      421 ccggaccata tgaagcagca tgacttcttc aagtccgcca tgccggaagg ctatgtgcag
      481 gaacgcacga tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt
      541 gaaggcgata ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa agaagacggc
      601 aatatcctgg gccataagct ggaatacaat tttaacagcc acaatgttta catcaccgcc
      661 gataaacaaa aaaatggcat taaagcgaat tttaaaattc gccacaacgt ggaggatggc
      721 agcgtgcagc tggctgatca ctaccagcaa aacactccaa tcggtgatgg tcctgttctg
      781 ctgccagaca atcactatct gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa
      841 cgcgatcata tggttctgct ggagttcgta accgcagcgg gcatcacgca tggtatggat
      901 gaactgtaca aatgatgata atactagagc caggcatcaa ataaaacgaa aggctcagtc
      961 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1021 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagaggatg accccatctg
     1081 gaatcgccgc ctgccttgcg ctatagcggc gaccctgatt ttccccatct aaaaataaat
     1141 aggggcctcg cttacatgcc gatcaagtac aagcctgaaa tccagcactc cgatttcaag
     1201 gacctgacca acctgatcca cttccagagc atggaaggca agatctggct tggcgaacag
     1261 cgcatgctgt tgcttcagtt ttcagcgatg gccagctttc gccgggaaat ggtcaatacc
     1321 ctgggcatcg aacgcgccaa gggcttgttc ctgcgccatg gttaccagtc cggcctgaag
     1381 gatgccgaac tggccaggaa gctgagaccg aatgccagcg aagtcggcat gttcctcgct
     1441 gggccgcaga tgcattcact caagggtctg gtcaaggtcc gccccaccga gctcgatatc
     1501 gacaaggaat acgggcgctt ctatgccgag atggagtgga tcgactcctt cgaggtggaa
     1561 atctgccaga ccgacctggg gcagatgcaa gacccggtgt gctggactct gctcggctac
     1621 gcctgcgcct attcctcggc gttcatgggc cgggaaatca tcttcaagga agtcagctgc
     1681 ggcggctgcg gcggcgacaa gtgccgggtc attggcaagc cggccgaaga gtgggacgac
     1741 gttgccagct tcaaacagta tttcaagaac gaccccatca tcgaggaact ctacgagttg
     1801 caatcgcaac tgttgtcgct gcgtaccaac ctcgacaaac aggaaggcca gtactacggc
     1861 atcggtcaga ccccggccta ccagaccgtg cgcaatatga tggacaaggc cgcacagggc
     1921 aaagtctcgg tgctgctgct tggcgagacc ggggtcggca aggaggtcat cgcgcgtagc
     1981 gtgcacctgc gcagcaaacg cgccgccgag ccctttgtcg cggtgaactg tgcggcgatc
     2041 ccgccggacc tgatcgagtc cgaattgttc ggcgtggaaa aaggcgcctt caccggcgcc
     2101 tcccagtcac gcatgggccg cttcgagcgg gccgacaagg gcaccatctt ccttgacgag
     2161 gtgatcgaac tcagcccgcg cgctcaggcc agtctgctgc gcgtgctgca agaaggcgag
     2221 ctggagcgag ttggcgacaa ccgcacgcgc aagatcgacg taagggttat cgccgccacc
     2281 cacgaggacc tggccgaagc ggtcaaggcc gggcgttttc gcgccgacct gtactaccga
     2341 ctgaacgttt tcccggtggc gatcccggcg ttgcgcgaac gccgcgagga cattccactg
     2401 ctggttgagc acttccttca gcgcttccac caggagtacg gcaagagaac cctcggcctt
     2461 tcagacaaag ccctggaggc ctgcctgcat tacagttggc cgggcaatat ccgtgagctg
     2521 gagaacgtca tcgagcgcgg catcatcctc accgatccga acgaaagcat cagcgtgcag
     2581 gcgctgttcc tacgggcgcc ggaagagccg cagaccgcca gcgagcgggt gtcgttggac
     2641 ggcgtgctga ttcagccagg caatggccag ggcagttgga tcagccagtt gttgagcagc
     2701 ggcctgagcc tcgacgagat cgaggaaagc ctgatgcgcg aagccatgca acaggccaac
     2761 caaaacgtct ccggtgccgc gcgcttgctc ggcctaagcc gaccggcact ggcctatcgg
     2821 ctgaagaaaa tcggcatcga aggctag
//