LOCUS       BBa_K1413022            1079 bp    DNA     linear       25-Nov-2024
DEFINITION  J23114-B0034-bphR2 mutated-B0015
ACCESSION   BBa_K1413022
VERSION     BBa_K1413022.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23114
     misc_feature    40..43
                     /label=conserved
     RBS             36..47
                     /label=BBa_B0034
     CDS             48..950
                     /label=bphR2
     misc_feature    189..194
                     /label=mutation
     CDS             48..950
                     /label=BBa_K1413021
     stem_loop       962..1005
                     /label=stem_loop
     misc_feature    951..1030
                     /label=BBa_B0010
     terminator      951..1030
                     /label=BBa_B0010
     stem_loop       1046..1065
                     /label=T7 TE
     misc_feature    1072..1072
                     /label=stop
     polyA_site      1066..1079
                     /label=polya
     misc_feature    1039..1079
                     /label=BBa_B0012
     terminator      1039..1079
                     /label=BBa_B0012
     terminator      951..1079
                     /label=BBa_B0015
ORIGIN
        1 tttatggcta gctcagtcct aggtacaatg ctagcaaaga ggagaaaatg gaactccacg
       61 acctggattt aaacctgctg gtagtattca accagttgat ggtcgacaag cgtgtctcta
      121 tcgtcgcgca aagcctaggc ctaacccaac ctgccgtaag caacgccctg aagcgcctgc
      181 gcaccgcact acaggacgaa ctgttcgtgc gtacccacca aggcatggaa cctacgccct
      241 atgctccaca tttagctgag cctatcgccc atgccatgca cagtctgcgc gaagcgctac
      301 atcacgaaga gcgcttcgat cctttaacca gcgagcgtac cttcaccttg gccatgaccg
      361 acattggtga gatatatttc atgccgcggt taatagatgc gctcgctcgt aaggcccccc
      421 attgcacaat cagcacggta cgcggcagct cggtgagttt gggacagtcg ttacaagacg
      481 gtacagtaga ttttgccgta ggcctgctac ccaacctaca ggccggcttc ttacagcgtc
      541 ggcttcttca taaccgctac gtgtgcttgt gccgtaagaa ccacccggcc accagagagc
      601 cactgacctt ggagcgcttc tgtgcctaca gccatatacg tgtcatcgcc gccagcactg
      661 gccacggcga agtagattcc cttatggcgc gagctggcat ccggcgggac attcgcctag
      721 aggttccgca cttcgtcgcc gtcggccata tcctccagca tacggagcta ctcgccaccg
      781 tacctgagcc gttcgctgac tgctgtgtag agcccttcgg cttgaatgtg ctgccaatcc
      841 ccatcgacct accagaaatc cccattaaca tgttctggca cgcaaaatat cacaaggatt
      901 tagccaacat ctggttgcga caactgatgt tcgagctgtt ttcggattga ccaggcatca
      961 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     1021 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//