LOCUS       BBa_K1413023            1149 bp    DNA     linear       09-Dec-2025
DEFINITION  Promoter-RBS-bphr2 mutated-Terminator
ACCESSION   BBa_K1413023
VERSION     BBa_K1413023.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23114
     misc_feature    44..51
                     /label=BBa_G0001
     misc_feature    64..67
                     /label=conserved
     RBS             60..71
                     /label=BBa_B0034
     misc_feature    80..87
                     /label=BBa_G0001
     CDS             94..996
                     /label=bphR2
     misc_feature    235..240
                     /label=mutation
     CDS             94..996
                     /label=BBa_K1413021
     misc_feature    1005..1012
                     /label=BBa_G0001
     stem_loop       1032..1075
                     /label=stem_loop
     misc_feature    1021..1100
                     /label=BBa_B0010
     terminator      1021..1100
                     /label=BBa_B0010
     stem_loop       1116..1135
                     /label=T7 TE
     misc_feature    1142..1142
                     /label=stop
     polyA_site      1136..1149
                     /label=polya
     misc_feature    1109..1149
                     /label=BBa_B0012
     terminator      1109..1149
                     /label=BBa_B0012
     terminator      1021..1149
                     /label=BBa_B0015
ORIGIN
        1 tttatggcta gctcagtcct aggtacaatg ctagctacta gagctctagt atactagaga
       61 aagaggagaa atactagagc tctagtatac tagatggaac tccacgacct ggatttaaac
      121 ctgctggtag tattcaacca gttgatggtc gacaagcgtg tctctatcgt cgcgcaaagc
      181 ctaggcctaa cccaacctgc cgtaagcaac gccctgaagc gcctgcgcac cgcactacag
      241 gacgaactgt tcgtgcgtac ccaccaaggc atggaaccta cgccctatgc tccacattta
      301 gctgagccta tcgcccatgc catgcacagt ctgcgcgaag cgctacatca cgaagagcgc
      361 ttcgatcctt taaccagcga gcgtaccttc accttggcca tgaccgacat tggtgagata
      421 tatttcatgc cgcggttaat agatgcgctc gctcgtaagg ccccccattg cacaatcagc
      481 acggtacgcg gcagctcggt gagtttggga cagtcgttac aagacggtac agtagatttt
      541 gccgtaggcc tgctacccaa cctacaggcc ggcttcttac agcgtcggct tcttcataac
      601 cgctacgtgt gcttgtgccg taagaaccac ccggccacca gagagccact gaccttggag
      661 cgcttctgtg cctacagcca tatacgtgtc atcgccgcca gcactggcca cggcgaagta
      721 gattccctta tggcgcgagc tggcatccgg cgggacattc gcctagaggt tccgcacttc
      781 gtcgccgtcg gccatatcct ccagcatacg gagctactcg ccaccgtacc tgagccgttc
      841 gctgactgct gtgtagagcc cttcggcttg aatgtgctgc caatccccat cgacctacca
      901 gaaatcccca ttaacatgtt ctggcacgca aaatatcaca aggatttagc caacatctgg
      961 ttgcgacaac tgatgttcga gctgttttcg gattgatact agagctctag tatactagag
     1021 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1081 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1141 gcgtttata
//